Presence and distribution of serotonin immunoreactivity in the cyprids of the barnacle Balanus amphitrite

Gallus, Lorenzo; Ramoino, Paola; Faimali, Marco; Piazza, Veronica; Maura, Guido; Marcoli, Manuela

doi:10.4081/961

Cited by 12 publications

(15 citation statements)

References 28 publications

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“…According to Yamamoto (1996), serotonin induced pronounced larval settlement, whereas dopamine inhibited the settlement (Yamamoto, 1999). The immunohistochemical detection of serotonergic neurons both in brain and posterior ganglion of B. amphitrite cyprid and serotonin in the antennules and compound eyes (Gallus et al, 2005) are consistent with previous findings. The localization of ChAT-like molecules in the cement glands, together with the demonstration of acetylcholinesterase (AChE) activity in the setae of antennulae (Faimali et al, 2003), might indicate an involvement of ACh in the cyprid settlement process.…”

Section: Introductionsupporting

confidence: 80%

Presence and distribution of FMRFamide‐like immunoreactivity in the cyprid of the barnacle Balanus amphitrite (Cirripedia, crustacea)

Gallus

Ferrando

Bottaro

et al. 2009

Microscopy Res & Technique

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The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barnacle Balanus amphitrite were investigated using immunohistochemical methods. Barnacles are considered to be one of the most important constituents of animal fouling communities, and the cyprid stage is specialized for settlement and metamorphosis in to the sessile adult condition. FLPs immunoreactive (IR) neuronal cell bodies were detected in both the central and the peripheral nervous system. One bilateral group of neurons somata was immunodetected in the brain, and IR nerve fibers were observed in the neuropil area and optic lobes. Intense immunostaining was also observed in the frontal filament complex: frontal filament tracts leaving the optic lobes and projecting towards the compound eyes, swollen nerve endings in the frontal filament vesicles, and thin nerve endings in the external frontal filament. Thin IR nerve fibers were also present in the cement glands. Two pairs of neuronal cell bodies were immunodetected in the posterior ganglion; some of their axons appear to project to the cirri. FLPs IR neuronal cell bodies were also localized in the wall of the dilated midgut and in the narrow hindgut; their processes surround the gut wall and allow gut neurons to synapse with one another. Our data demonstrated the presence of FLPs IR substances in the barnacle cyprid. We hypothesize that these peptides act as integrators in the central nervous system, perform neuromuscular functions for thoracic limbs, trigger intestinal movements and, at the level of the frontal filament, play a neurosecretory role.

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Section: Introductionsupporting

confidence: 80%

Presence and distribution of FMRFamide‐like immunoreactivity in the cyprid of the barnacle Balanus amphitrite (Cirripedia, crustacea)

Gallus

Ferrando

Bottaro

et al. 2009

Microscopy Res & Technique

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“…The distribution of serotonin has been extensively mapped in a number of arthropods (e.g., Myhrberg et al 1979;Beltz and Kravitz 1983;Bishop and O'Shea 1983;Elofsson 1983Elofsson , 1992Cournil et al 1984;Klemm et al 1984;Nässel and Elekes 1984;Schürmann and Klemm 1984;Nässel and Elekes 1985;Longley and Longley 1986;Siwicki et al 1987;Sandeman et al 1988;Dubbels and Elofsson 1989;Homberg and Hildebrand 1989;Katz et al 1989;Beltz et al 1990;Real and Czternasty 1990;Salecker and Distler 1990;Lundell and Hirsh 1994;Thompson et al 1994;Christie et al 1995;Harzsch and Dawirs 1995;Schürmann et al 1995;Würden and Homberg 1995;Bicker 1999;Callaway and Stuart 1999;Hörner 1999;Leitinger et al 1999;Tierney et al 1999;Wegerhoff 1999;Ignell 2001;Homberg 2002;Moreau et al 2002;Harzsch 2004;Settembrini and Villar 2004;Fu et al 2005;Gallus et al 2005;Barthelemy et al 2006;…”

Section: Copepod Cns Contains Extensive Aminergic Innervationmentioning

confidence: 99%

Immunohistochemical mapping of histamine, dopamine, and serotonin in the central nervous system of the copepod Calanus finmarchicus (Crustacea; Maxillopoda; Copepoda)

Hartline

Christie

2010

Cell Tissue Res

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Calanoid copepods constitute an important group of marine planktonic crustaceans that often dominate the metazoan biomass of the world's oceans. In proportion to their ecological importance, little is known about their nervous systems. We have used immunohistochemical techniques in a common North Atlantic calanoid to localize re-identifiable neurons that putatively contain the biogenic amines histamine, dopamine, and serotonin. We have found low numbers of such cells and cell groups (approximately 37 histamine pairs, 22 dopamine pairs, and 12 serotonin pairs) compared with those in previously described crustaceans. These cells are concentrated in the anterior part of the central nervous system, the majority for each amine being located in the three neuromeres that constitute the brain (protocerebrum, deutocerebrum, and tritocerebrum). Extensive histamine labeling occurs in several small compact protocerebral neuropils, three pairs of larger, more posterior, paired, dense neuropils, and one paired diffuse tritocerebral neuropil. The most concentrated neuropil showing dopamine labeling lies in the putative deutocerebrum, associated with heavily labeled commissural connections between the two sides of the brain. The most prominent serotonin neuropil is present in the anterior medial part of the brain. Tracts of immunoreactive fibers of all three amines are prominent in the cephalic region of the nervous system, but some projections into the most posterior thoracic regions have also been noted.

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“…These were neither sections across the brain nor sections across the posterior ganglion. Based on the morphological description of the nervous system and serotonin distribution in A. amphitrite cyprids 19 20 21 , the regions of signals were circumesophageal connectives that connected the brain and posterior ganglion. In the longitudinal sections, signal spots were symmetrically localized near the carapace ( Fig.…”

Section: Resultsmentioning

confidence: 99%

p38 MAPK regulates PKAα and CUB-serine protease in Amphibalanus amphitrite cyprids

Zhang

Wong

et al. 2015

Sci Rep

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The MKK3-p38 MAPK pathway has been reported to mediate larval settlement in Amphibalanus (=Balanus) amphitrite. To clarify the underlying molecular mechanism, we applied label-free proteomics to analyze changes in the proteome of cyprids treated with a p38 MAPK inhibitor. The results showed that the expression levels of 80 proteins were significantly modified (p < 0.05). These differentially expressed proteins were assigned to 15 functional groups according to the KOG database and 9 pathways were significantly enriched. Further analysis revealed that p38 MAPK might regulate the energy supply and metamorphosis. Two potential regulatory proteins, CUB-serine protease and PKAα, were both down-regulated in expression. CUB-serine protease localized to postaxial seta 2 and 3, as well as the 4 subterminal sensilla in the antennule. Importantly, it was co-localized with the neuron transmitter serotonin in the sections, suggesting that the CUB-serine protease was present in the neural system. PKAα was highly expressed during the cyprid and juvenile stages, and it was co-localized with phospho-p38 MAPK (pp38 MAPK) to the cement gland, suggesting that PKAα might have some functions in cement glands. Overall, p38 MAPK might regulate multiple functions in A. amphitrite cyprids, including the energy supply, metamorphosis, neural system and cement glands.

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Presence and distribution of serotonin immunoreactivity in the cyprids of the barnacle Balanus amphitrite

Cited by 12 publications

References 28 publications

Presence and distribution of FMRFamide‐like immunoreactivity in the cyprid of the barnacle Balanus amphitrite (Cirripedia, crustacea)

Presence and distribution of FMRFamide‐like immunoreactivity in the cyprid of the barnacle Balanus amphitrite (Cirripedia, crustacea)

Immunohistochemical mapping of histamine, dopamine, and serotonin in the central nervous system of the copepod Calanus finmarchicus (Crustacea; Maxillopoda; Copepoda)

p38 MAPK regulates PKAα and CUB-serine protease in Amphibalanus amphitrite cyprids

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