Presence and Density of Helicobacter pylori Biofilms in Human Gastric Mucosa in Patients With Peptic Ulcer Disease

Coticchia, James M.; Sugawa, Choichi; Tran, Vivian; Gurrola, José; Kowalski, Evan; Carron, Michael A.

doi:10.1016/j.gassur.2005.12.009

Cited by 93 publications

(88 citation statements)

References 15 publications

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“…Biofilm formation in H. pylori-infected individuals is well documented (33)(34)(35)(36), and a recent publication identified biofilm formation in an animal model of infection (37). H. pylori colonizes the gastric niche, which is highly acidic; however, H. pylori is a neutrophile and employs several mechanism to neutralize the immediate environment (69).…”

Section: Figmentioning

confidence: 99%

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Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation

et al. 2016

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Helicobacter pylori must be able to rapidly respond to fluctuating conditions within the stomach. Despite this need for constant adaptation, H. pylori encodes few regulatory proteins. Of the identified regulators, the ferric uptake regulator (Fur), the nickel response regulator (NikR), and the two-component acid response system (ArsRS) are each paramount to the success of this pathogen. While numerous studies have individually examined these regulatory proteins, little is known about their combined effect. Therefore, we constructed a series of isogenic mutant strains that contained all possible single, double, and triple regulatory mutations in Fur, NikR, and ArsS. A growth curve analysis revealed minor variation in growth kinetics across the strains; these were most pronounced in the triple mutant and in strains lacking ArsS. Visual analysis showed that strains lacking ArsS formed large aggregates and a biofilm-like matrix at the air-liquid interface. Biofilm quantification using crystal violet assays and visualization via scanning electron microscopy (SEM) showed that all strains lacking ArsS or containing a nonphosphorylatable form of ArsR (ArsR-D52N mutant) formed significantly more biofilm than the wild-type strain. Molecular characterization of biofilm formation showed that strains containing mutations in the ArsRS pathway displayed increased levels of cell aggregation and adherence, both of which are key to biofilm development. Furthermore, SEM analysis revealed prevalent coccoid cells and extracellular matrix formation in the ArsR-D52N, ⌬nikR ⌬arsS, and ⌬fur ⌬nikR ⌬arsS mutant strains, suggesting that these strains may have an exacerbated stress response that further contributes to biofilm formation. Thus, H. pylori ArsRS has a previously unrecognized role in biofilm formation. IMPORTANCEDespite a paucity of regulatory proteins, adaptation is key to the survival of H. pylori within the stomach. While prior studies have focused on individual regulatory proteins, such as Fur, NikR, and ArsRS, few studies have examined the combined effect of these factors. Analysis of isogenic mutant strains that contained all possible single, double, and triple regulatory mutations in Fur, NikR, and ArsS revealed a previously unrecognized role for the acid-responsive two-component system ArsRS in biofilm formation.

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Section: Figmentioning

confidence: 99%

“…Biofilm formation by H. pylori has been demonstrated both in vitro (30)(31)(32) and in vivo (33)(34)(35)(36)(37). While it is unclear exactly what drives biofilm formation in H. pylori, protection from antibiotics and host defenses during infection (35,38,39) and/or protection in putative environmental reservoir (40-43) have been proposed.…”

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confidence: 99%

Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation

et al. 2016

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“…These bacilli produce large amounts of extracellular exopoly saccharides (EPS), which protect bacteria against pH changes, allowing them to process the transmission and colonization [12]. Thanks to matrix composed of exopolysaccharides, biofilm is flexible and very durable [13].…”

Section: Factors Influencing Helicobacter Pylori Biofilm Formation Inmentioning

confidence: 99%

“…Coticchia et al [13], analyzing endoscopic biopsies taken from the oesophagus, stomach and duodenum, collected from people with positive (study group) and negative (control group) rapid urease test results, found that H. pylori has the ability to form biofilm in vivo. As a result of analysis of the specimens, it was found that the average percentage of surface area covered with a mucosal biofilm in the patients was 97.3%, while in the control group it was only 1.6%.…”

Section: Role Of Helicobacter Pylori Biofilm In Digestive Tract Diseasementioning

confidence: 99%

The role of biofilm formation in pathogenesis of Helicobacter pylori infections

Bińkowska¹,

Biernat²,

Duś³

et al. 2013

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K Ke ey y w wo or rd ds s: : biofilm, Helicobacter pylori, infections, gastrointestinal tract. S Sł ło ow wa a k kl lu uc cz zo ow we e: : biofilm, Helicobacter pylori, zakażenia, przewód pokarmowy.A Ad dd dr re es ss s f fo or r c co or rr re es sp po on nd de en nc ce e: : Aldona Bińkowska, Department of Microbiology, Wroclaw Medical University, 4 Chałubińskiego St, 50-368 Wroclaw, Poland, phone: +48 71 784 12 88, e-mail: aldona.binkowska@gmail.com Review paper/Artykuł poglądowy AbstractThe significance of biofilm in pathogenesis of Helicobacter pylori (H. pylori) infections is poorly understood. Biofilm is thought to play a role in transmission of infection to humans, may influence the nature of inflammatory changes in the gastrointestinal tract, and may cause reinfection. In this study, the forms of life of H. pylori, biofilm formation in the environment and human gastrointestinal tract and its impact on the course of infection as well as difficulties in elimination of infections accompanied with biofilm are discussed. StreszczenieZnaczenie biofilmu w patogenezie zakażeń pałeczkami Helicobacter pylori (H. pylori) nie jest do końca poznane. Uważa się, że biofilm może odgrywać rolę w transmisji zakażenia tymi drobnoustrojami, a także wpływać na charakter zmian zapalnych w przewodzie pokarmowym człowieka i przyczyniać się do ponownej infekcji. W pracy omówiono formy bytowania H. pylori, tworzenie się biofilmu w środowisku zewnętrznym i w przewodzie pokarmowym człowieka, jego wpływ na przebieg zakażenia oraz trudności w eliminacji zakażeń przebiegających z tworzeniem biofilmu.

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“…Helicobacter pylori is a microorganism with a pronounced capability of adaptation under environmental stress solicitations [3]. Its persistence and antimicrobial resistance to the drugs commonly used in the anti-H. pylori therapy are associated with genetic variability [4] and biofilm development [5][6][7][8]. Helicobacter pylori is capable of forming a mature biofilm characterized by clustered bacterial cells arranged in an abundant matrix after 3 days of incubation in vitro [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Alginate Lyase on Biofilm-GrownHelicobacter pyloriProbed by Atomic Force Microscopy

Maiorana

Bugli

Papi

et al. 2015

International Journal of Polymer Science

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Helicobacter pylori (H. pylori) is a microorganism with a pronounced capability of adaptation under environmental stress solicitations. Its persistence and antimicrobial resistance to the drugs commonly used in the anti-H. pylori therapy are associated with the development of a biofilm mainly composed of DNA, proteins, and polysaccharides. A fundamental step to increase the success of clinical treatments is the development of new strategies and molecules able to interfere with the biofilm architecture and thus able to enhance the effects of antibiotics. By using Atomic Force Microscopy and Scanning Electron Microscopy we analyzed the effects of the alginate lyase (AlgL), an enzyme able to degrade a wide class of polysaccharides, on the H. pylori shape, surface morphology, and biofilm adhesion properties. We demonstrated that AlgL generates a noticeable loss of H. pylori coccoid form in favor of the bacillary form and reduces the H. pylori extracellular polymeric substances (EPS).

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Presence and Density of Helicobacter pylori Biofilms in Human Gastric Mucosa in Patients With Peptic Ulcer Disease

Cited by 93 publications

References 15 publications

Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation

Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation

The role of biofilm formation in pathogenesis of Helicobacter pylori infections

Effect of Alginate Lyase on Biofilm-GrownHelicobacter pyloriProbed by Atomic Force Microscopy

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