Presence and Analysis of Plasmids in Human and Animal Associated Arcobacter Species

Douidah, Laid; Zutter, Lieven De; Nieuwerburgh, Filip Van; Deforce, Dieter; Ingmer, Hanne; Vandenberg, Olivier; Abeele, Anne‐Marie Van den; Houf, Kurt

doi:10.1371/journal.pone.0085487

Cited by 26 publications

(18 citation statements)

References 38 publications

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“…Our results suggest Arcobacter members as potential keystone players involved in conjugative plasmid acquisition and plasmid shuttling through WWTPs, before potential redistribution to broader phylogenetic recipients from both Gram‐types, including freshwater copiotrophs. This is supported by the literature, as Arcobacter ‐related members were reported to be associated with conjugative plasmid transfer (Douidah et al., ), antibiotic resistance (Zacharow et al., ) and survival to changing environmental conditions (Van Driessche & Houf, ).…”

Section: Discussionsupporting

confidence: 65%

Deciphering conjugative plasmid permissiveness in wastewater microbiomes

et al. 2017

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Wastewater treatment plants (WWTPs) are designed to robustly treat polluted water. They are characterized by ceaseless flows of organic, chemical and microbial matter, followed by treatment steps before environmental release. WWTPs are hotspots of horizontal gene transfer between bacteria via conjugative plasmids, leading to dissemination of potentially hazardous genetic material such as antimicrobial resistance genes (AMRGs). While current focus is on the threat of AMRGs spreading and their environmental maintenance, conjugative plasmid transfer dynamics within and between bacterial communities still remains largely uncharted. Furthermore, current in vitro methods used to assess conjugation in complex microbiomes do not include in situ behaviours of recipient cells, resulting in partial understanding of transfers. We investigated the in vitro conjugation capacities of WWTP microbiomes from inlet sewage and outlet treated water using the broad-host range IncP-1 conjugative plasmid, pKJK5. A thorough molecular approach coupling metagenomes to 16S rRNA DNA/cDNA amplicon sequencing was established to characterize microbiomes using the ecological concept of functional response groups. A broad diversity of recipient bacterial phyla for the plasmid was observed, especially in WWTP outlets. We also identified permissive bacteria potentially able to cross WWTPs and engage in conjugation before and after water treatment. Bacterial activity and lifestyle seem to influence conjugation extent, as treated water copiotrophs were the most represented strategist amongst transconjugants. Correlation analysis highlighted possible plasmid transmission routes into communities between the sewage to the environment, with identification of keystone members (e.g., Arcobacter) potentially involved in cross-border exchanges between distant Gram-positive and Gram-negative phyla.

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Section: Discussionsupporting

confidence: 65%

Deciphering conjugative plasmid permissiveness in wastewater microbiomes

et al. 2017

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“…Jacquiod et al 53 described Arcobacter from wastewater treatment plants as a keystone player involved in shuttling antibiotic resistance genes between distant gram-positive and gram-negative phyla. It has also been reported that the Arcobacter spp are associated with conjugative plasmid transfer 54 and survival in changing environmental conditions 55 . The second most abundant bacterial species detected in the Pinheiros River is the well-characterized exoelectrogenic Geobacter_uc from the δ-Proteobacteria class 56 .…”

Section: Discussionmentioning

confidence: 98%

Bacterial community composition and potential pathogens along the Pinheiros River in the southeast of Brazil

Godoy

Marcondes

Pessôa

et al. 2020

Sci Rep

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The Pinheiros River in São Paulo, Brazil, crosses through the capital city and has its confluence with the River Tiete, which comprises several reservoirs along its course. Although Pinheiros River is considered one of the heaviest polluted rivers in Brazil, little is known about its bacterial composition, their metabolic functions or how these communities are affected by the physicochemical parameters of the river. In this study, we used the 16S rRNA gene Illumina MiSeq sequencing to profile the bacterial community from the water surface at 11 points along the course of the River. Taxonomical composition revealed an abundance of Proteobacteria phyla, followed by Firmicutes and Bacteroidetes, with a total of 233 classified bacterial families and 558 known bacterial genera. Among the 35 potentially pathogenic bacteria identified, Arcobacter was the most predominant genus. The disrupted physicochemical parameters detected in this study may possibly contribute to the composition and distribution of the bacterial community in the Pinheiros River. Predictive functional analysis suggests the River is abundant in motility genes, including bacterial chemotaxis and flagellar assembly. These results provide novel and detailed insights into the bacterial communities and putative function of the surface water in the Pinheiros River.

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“…Described resistance determinants are mostly located chromosomal, and no antimicrobial resistance genes have been identified on plasmids, yet. Until now, only a few studies have reported the presence of plasmids in the genus Aliarcobacter ( Harrass et al, 1998 ; Douidah et al, 2014 ; On et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Genomic Analysis and Antimicrobial Resistance of Aliarcobacter cryaerophilus Strains From German Water Poultry

Müller

Hotzel

Ahlers³

et al. 2020

Front. Microbiol.

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Aliarcobacter cryaerophilus (formerly Arcobacter cryaerophilus) is a globally emerging foodborne and zoonotic pathogen. However, little is known about the species' genomic features and diversity, antibiotic resistance and virulence. In this study, 27 A. cryaerophilus strains from water poultry in Thuringia, Germany, were investigated using whole-genome sequencing. Four of these strains were sequenced using longand short-read sequencing methods to obtain circularized genomes. The German strains belong to the A. cryaerophilus cluster I. Cluster I genomes exhibited a high degree of genetic diversity in which variable sites comprised 9.1% of the core genome. The German strains formed three subgroups that contained 2, 6, and 9 strains, respectively. The genomic analysis of cluster I revealed variable presence of mobile elements and that 65% of the strains lack CRISPR systems. The four circularized genomes carried a ∼2 Mbp chromosome and a single megaplasmid (size 98.1-154.5 Kbp). The chromosome was densely packed with coding sequences (∼92%) and showed inversions and shifts in the gene blocks between different strains. Antimicrobial resistance was assessed using a gradient strip diffusion method and showed that all 27 strains were resistant to cefotaxime and susceptible to erythromycin, gentamicin, and ampicillin. Sixteen strains were also resistant to ciprofloxacin, whereas 23 were resistant to streptomycin. The genetic prediction of antibiotic resistance identified numerous efflux pumps similar to those found in A. butzleri. All strains harbored two beta-lactamase genes which may explain the cefotaxime resistance. A correlation between the gyrA point mutation (Thr-85-Ile) and ciprofloxacin resistance was partially discovered in 15 out of 16 strains. In silico virulence profiling showed a wide range of virulence factors including a full chemotaxis system and most of the flagellar genes. In contrast to A. butzleri, no urease cluster was found. This study provides new insights into the genomic variability of A. cryaerophilus strains of cluster I. The different genetic makeup of these strains may contribute to the virulence of strains and the severity of the infections in humans.

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Presence and Analysis of Plasmids in Human and Animal Associated Arcobacter Species

Cited by 26 publications

References 38 publications

Deciphering conjugative plasmid permissiveness in wastewater microbiomes

Deciphering conjugative plasmid permissiveness in wastewater microbiomes

Bacterial community composition and potential pathogens along the Pinheiros River in the southeast of Brazil

Genomic Analysis and Antimicrobial Resistance of Aliarcobacter cryaerophilus Strains From German Water Poultry

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