Presence and Analysis of Large Plasmids in Oenococcus oeni

Brito, Luísa; Paveia, H.

doi:10.1006/plas.1999.1397

Cited by 15 publications

(9 citation statements)

References 28 publications

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“…Although it has been shown that O. oeni is able to receive foreign DNA by transformation in vitro (5) and by conjugation (49), there is no evidence for horizontal gene transfer in vivo. The existence of recombination is not unexpected, because the presence of plasmids (1,46), bacteriophages, and insertion sequences (48) was previously shown in O. oeni. Zé-Zé et al (48) reported that small differences observed in two O. oeni strains are apparently due to insertion or deletion events related to the presence of insertion sequences.…”

Section: Discussionmentioning

confidence: 78%

Allelic Diversity and Population Structure inOenococcus oenias Determined from Sequence Analysis of Housekeeping Genes

Rivas

Marcobal

Múñoz

2004

Appl Environ Microbiol

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Oenococcus oeni is the organism of choice for promoting malolactic fermentation in wine. The population biology of O. oeni is poorly understood and remains unclear. For a better understanding of the mode of genetic variation within this species, we investigated by using multilocus sequence typing (MLST) with the gyrB, pgm, ddl, recP, and mleA genes the genetic diversity and genetic relationships among 18 O. oeni strains isolated in various years from wines of the United States, France, Germany, Spain, and Italy. These strains have also been characterized by ribotyping and restriction fragment length polymorphism (RFLP) analysis of the PCRamplified 16S-23S rRNA gene intergenic spacer region (ISR). Ribotyping grouped the strains into two groups; however, the RFLP analysis of the ISRs showed no differences in the strains analyzed. In contrast, MLST in oenococci had a good discriminatory ability, and we have found a higher genetic diversity than indicated by ribotyping analysis. All sequence types were represented by a single strain, and all the strains could be distinguished from each other because they had unique combinations of alleles. Strains assumed to be identical showed the same sequence type. Phylogenetic analyses indicated a panmictic population structure in O. oeni. Sequences were analyzed for evidence of recombination by split decomposition analysis and analysis of clustered polymorphisms. All results indicated that recombination plays a major role in creating the genetic heterogeneity of O. oeni. A low standardized index of association value indicated that the O. oeni genes analyzed are close to linkage equilibrium. This study constitutes the first step in the development of an MLST method for O. oeni and the first example of the application of MLST to a nonpathogenic food production bacteria.Oenococcus oeni, formerly Leuconostoc oenos (7), is the species of lactic acid bacteria (LAB) most frequently associated with malolactic fermentation (MLF) in wine. MLF, which occurs after alcoholic fermentation during wine making, is induced by the growth of LAB. Normally, spontaneous MLF takes place when LAB develop in wine after alcoholic fermentation. However, when fermentation by indigenous bacteria is relied upon, the fitness of the bacteria present to carry out MLF may be highly variable, and consequently their winemaking properties are unpredictable. In order to exercise greater control over wine-making processes, common winemaking practices therefore involve inoculation of wine with either commercially prepared strains or in-house winery strains of malolactic bacteria (4). Therefore, the differentiation of O. oeni strains at the strain level becomes a major concern, since their adaptation to wine and influence on organoleptic quality are strain specific (2). Moreover, manufacturers of malolactic starters need accurate control of their products, and winemakers have to be able to recognize the inoculated strains during vinification. The need for positive identification of different isolates is also acknowled...

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Section: Discussionmentioning

confidence: 78%

Allelic Diversity and Population Structure inOenococcus oenias Determined from Sequence Analysis of Housekeeping Genes

Rivas

Marcobal

Múñoz

2004

Appl Environ Microbiol

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“…The nature and origin of such plasmids is so far unknown. DNA plasmids of varying sizes were detected in some oenoccocal strains (Brito and Paveia, 1999;Zé-Zé et al, 2008). However southern hybridization using these endogenous oenococcal plasmids as probes against PFGE profiles ruled out the presence of similar integrated plasmids or remnants of plasmid DNA in the chromosomes of the tested strains (Zé-Zé et al, 2008).…”

Section: Discussionmentioning

confidence: 93%

IS30 elements are mediators of genetic diversity in Oenococcus oeni

Gharniti¹,

Dols‐Lafargue²,

Bon³

et al. 2012

International Journal of Food Microbiology

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“…El interés comercial de esta bacteria como cultivo iniciador en procesos de vinificación ha potenciado su estudio en los últimos 15 años, centrándose las investigaciones en tres aspectos básicos, la secuenciación de su genoma (Klaenhammer et al, 2002;Mills, 2004), la genética extracromosomal (Fremaux et al, 1993;Brito et al, 1996;Zúñiga et al, 1996;Alegre et al, 1999;Brito & Paveia, 1999;Mesas et al, 2001;Mesas et al, 2004) y el comportamiento de O. oeni bajo las condiciones de estrés que le proporciona el vino, su hábitat natural (Masqué et al, 1991;Guzzo et al, 1998;Vidal et al, 2001;Teixeira et al, 2002;G-Alegría et al, 2004).…”

Section: Introductionunclassified