Preprophase bands in cultured multinucleate soybean protoplasts

Wang, H.; Cutler, Adrian J.; Fowke, Larry C.

doi:10.1007/bf01403666

Cited by 16 publications

(3 citation statements)

References 13 publications

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“…The pattern offluorescence was remarkably similar to that of MTs in other dividing plant cells, as determined by a variety of techniques, including especially indirect immunofluorescence with anti-tubulin antibodies (5,6,(18)(19)(20)(21) and electron microscopy (1,(22)(23)(24). We conclude, therefore, that the brain tubulin copolymerizes with the plant cell MTs.…”

Section: Resultssupporting

confidence: 70%

Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin.

Zhang

Wadsworth

Hepler

1990

Proc. Natl. Acad. Sci. U.S.A.

175

105

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Carboxyfluorescein-labeled brain tubulin has been microinjected into stamen hair cells of Tradescantia, and its distribution during mitosis and cytokinesis was examined using confocal laser scanning fluorescence microscopy. The results show that brain tubulin incorporates into plant microtubules and is utilized throughout mitosis and cytokinesis. Microtubule structures that incorporate brain tubulin include the preprophase band, the perinuclear sheath at late prophase, the kinetochore fibers during prometaphase, metaphase, and anaphase, the interzone spindle during anaphase, and finally the phragmoplast during late anaphase and telophase. All of these microtubule-containing structures and, notably, their transitions from one to another have been observed in single live cells progressing through mitosis and cytokinesis.

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Section: Resultssupporting

confidence: 70%

Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin.

Zhang

Wadsworth

Hepler

1990

Proc. Natl. Acad. Sci. U.S.A.

175

105

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show abstract

“…In insect spennatocytes, a well-organized spindle can fonn without the presence of a centrosome or detectable pericentriolar material at one pole (177), which implies that modes of spindle organization without typical centrosomes can also occur in animal cells. When there is more than one nucleus (or micronucleus) per cell, each is able to fonn a spindle (or microspindle) (30, 41, 175,191,193); therefore, the organizing material must be flexible enough to become distributed over more than one nucleus . When there is more than one nucleus (or micronucleus) per cell, each is able to fonn a spindle (or microspindle) (30, 41, 175,191,193); therefore, the organizing material must be flexible enough to become distributed over more than one nucleus .…”

Section: Problems Of Microtubule Organizationmentioning

confidence: 99%

The Structure and Function of the Mitotic Spindle in Flowering Plants

Baskin

Cande

1990

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

161

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“…The preferential formation of the single PPB around the nucleus possessing the largest cytoplasmic environment and the formation of two PPBs in the longest binucleate cells indicate that tubulin or related proteins are a limiting factor for PPB formation. This also seems to occur in multinucleate soybean protoplasts (Wang et al 1989).…”

Section: Discussionmentioning

confidence: 83%