Preprogrammed Dynamic Microstructured Polymer Interfaces

Tokarev, Ihor; Minko, Sergiy

doi:10.1002/adfm.201903478

Cited by 15 publications

(16 citation statements)

References 206 publications

(75 reference statements)

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“…86 Thus, the pH and thermosensitive PPMs had tunable the pores size, which is beneficial for improving the enzymolysis efficiency based on the nanopore confinement effect. 93,98 P(AA) can be grafted onto the layered polyelectrolyte films. 92 On the other hand, P(AA) as a polyanion and poly(allylamine) [P(AH)] as a polycation 82 can also be modified within the pore-wall by the LBL-assembling method.…”

Section: Strategies For Generating Sr-ppmsmentioning

confidence: 99%

“…Physical stimuli possess several intrinsic advantages over chemical stimili: noncontact, tunable strength, and temporal and spatial resolution, the SR-PPMs undergo either a reversible or irreversible modification because of bonding, hydrophobic, or hydrophilic interactions, leading to changes in structure or self-assembly at large-length scales. Ideal SR-PPMs can be fabricated via functionalization of the film surface or pores wall (Figure ) and LBL-assembling methods to improve the surface hydrophilicity and to tune the surface charge, which is beneficial for enzyme immobilization. − …”

Section: Strategies For Generating Sr-ppmsmentioning

confidence: 99%

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Design of Switchable Enzyme Carriers Based on Stimuli-Responsive Porous Polymer Membranes for Bioapplications

Qiao

2021

ACS Appl. Bio Mater.

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Design of efficient enzyme carriers, where enzymes are conjugated to supports, has become an attractive research avenue. Immobilized enzymes are advantageous for practical applications because of their convenience in handling, ease of separation, and good reusability. However, the main challenge is that these traditional enzyme carriers are unable to regulate the enzymolysis efficiency or to protect the enzymes from proteolytic degradation, which restricts their effectiveness of enzymes in bioapplications. Enlightened by the stimuli-responsive channels in the natural cell membranes, conjugation of the enzymes within flat-sheet stimuli-responsive porous polymer membranes (SR-PPMs) as artificial cell membranes is an efficient strategy for circumventing this challenge. Controlled by the external stimuli, the multifunctional polymer chains, which are incorporated within the membranes and attached to the enzyme, change their structures to defend the enzyme from the external environmental disturbances and degradation by proteinases. Specifically, smart SR-PPM enzyme carriers (SR-PPMECs) not only permit convective substrate transfer through the accessible porous network, dramatically improving enzymolysis efficiency due to the adjustable pore sizes and the confinement effect, but they also act as molecular switches for regulating its permeability and selectivity. In this review, the concept of SR-PPMECs is presented. It covers the latest developments in design strategies of flat-sheet SR-PPFMs, fabrication protocols of SR-PPFMECs, strategies for the regulation of enzymolysis efficiency, and their cutting-edge bioapplications.

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Section: Strategies For Generating Sr-ppmsmentioning

confidence: 99%

Section: Strategies For Generating Sr-ppmsmentioning

confidence: 99%

Design of Switchable Enzyme Carriers Based on Stimuli-Responsive Porous Polymer Membranes for Bioapplications

Qiao

2021

ACS Appl. Bio Mater.

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“…In some cases, a polymer brush may switch between an attracting and a repelling state with respect to a certain biomolecule, depending on factors in the liquid environment such as temperature, [4] salt content [5] or pH [5] . In principle, this is highly interesting for applications as it creates responsive interfaces [1a,d,f] that may be used to capture and subsequently release proteins for instance.…”

Section: Introductionmentioning

confidence: 99%

Electrically Switchable Polymer Brushes for Protein Capture and Release in Biological Environments**

et al. 2022

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Interfaces functionalized with polymers are known for providing excellent resistance towards biomolecular adsorption and for their ability to bind high amounts of protein while preserving their structure. However, making an interface that switches between these two states has proven challenging and concepts to date rely on changes in the physiochemical environment, which is static in biological systems. Here we present the first interface that can be electrically switched between a high-capacity (> 1 μg cm À 2 ) multilayer protein binding state and a completely non-fouling state (no detectable adsorption). Switching is possible over multiple cycles without any regeneration. Importantly, switching works even when the interface is in direct contact with biological fluids and a buffered environment. The technology offers many applications such as zero fouling on demand, patterning or separation of proteins as well as controlled release of biologics in a physiological environment, showing high potential for future drug delivery in vivo.

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“…Various stimuli allow controlling appropriate biodegradation profiles: as it was shown, for example, for polymers suspended in a solution [16], chemical triggering for bringing polymers together [17] or laser remote release of materials immobilized on bio-coating [18-20] through a localized temperature rise [21][22][23].The addition of particles with encapsulated biomolecules allowed the implementation of the concept of both hardness modification and drug delivery with the coatings [24,25]. Such modification of the coatings can be well suited for applications in pharmacology [26], where delivery of molecules is just as essential as control over physico-chemical properties of films [27,28] It is interesting to note that functionalization of hybrid assemblies [29] can be made with micro-or nano-particles, where the former size is more suited for thicker polymeric films and hydrogels [30,31], while smaller nanoparticles are better suited for tuning mechanical properties of less gelly films [32]. It is envisioned that the distribution [33] of such particles will allow to fully control the softness-hardness of films and other physico-chemical properties.In addition to inorganic particles, various capsules provide the means of yet another type of modification of polymer-based films to promote the interaction with cells.…”

mentioning

confidence: 99%

“…The addition of particles with encapsulated biomolecules allowed the implementation of the concept of both hardness modification and drug delivery with the coatings [24,25]. Such modification of the coatings can be well suited for applications in pharmacology [26], where delivery of molecules is just as essential as control over physico-chemical properties of films [27,28] It is interesting to note that functionalization of hybrid assemblies [29] can be made with micro-or nano-particles, where the former size is more suited for thicker polymeric films and hydrogels [30,31], while smaller nanoparticles are better suited for tuning mechanical properties of less gelly films [32]. It is envisioned that the distribution [33] of such particles will allow to fully control the softness-hardness of films and other physico-chemical properties.…”

mentioning

confidence: 99%

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

2020

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In this review, materials based on polymers and hybrids possessing both organic and inorganic contents for repairing or facilitating cell growth in tissue engineering are discussed. Pure polymer based biomaterials are predominantly used to target soft tissues. Stipulated by possibilities of tuning the composition and concentration of their inorganic content, hybrid materials allow to mimic properties of various types of harder tissues. That leads to the concept of "one-matches-all" referring to materials possessing the same polymeric base, but different inorganic content to enable tissue growth and repair, proliferation of cells, and the formation of the ECM (extra cellular matrix). Furthermore, adding drug delivery carriers to coatings and scaffolds designed with such materials brings additional functionality by encapsulating active molecules, antibacterial agents, and growth factors. We discuss here materials and methods of their assembly from a general perspective together with their applications in various tissue engineering sub-areas: interstitial, connective, vascular, nervous, visceral and musculoskeletal tissues. The overall aims of this review are two-fold: (a) to describe the needs and opportunities in the field of bio-medicine, which should be useful for material scientists, and (b) to present capabilities and resources available in the area of materials, which should be of interest for biologists and medical doctors.Polymers 2020, 12, 620 2 of 30 allows to tailor functionalize the coatings, where their responsiveness to external stimuli is one of their biggest advantages.Polymers stand out as a special class of materials due to the flexibility of design of their blocks and various functionalities, which is brought by their versatile assembly or by introducing additional side-groups or blocks, in the case of block-co-polymers. In this regard, both synthetic and biocompatible polymers are available, and polymer engineering represents a growing area tailoring the needs often driven by applications. With all advantages and the flexibility of the design, there is one significant weakness of polymers-the softness. In regard with tissue engineering, that translates to the fact that some materials can match predominantly soft tissue, but it is difficult to tune mechanical properties of polymers to match the hardness of bones, tendons, etc. And since mechanical properties of materials affect and even drive cell and tissue growth, enhancement of mechanical properties of polymers and biomaterials is essential. Chemical crosslinking can be applied to address these challenges, but that solves problems only partially.To solve these challenges the so-called hybrid materials [6] are used, which possess both inorganic and organic contents. Increasingly, hybrid materials are used in designing the extracellular matrix. The hybrid matrix design for various tissue engineering applications should meet bio-medical requirements. On the one hand, it needs to be biocompatible and biodegradable, which is achieved through the po...

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Preprogrammed Dynamic Microstructured Polymer Interfaces

Cited by 15 publications

References 206 publications

Design of Switchable Enzyme Carriers Based on Stimuli-Responsive Porous Polymer Membranes for Bioapplications

Design of Switchable Enzyme Carriers Based on Stimuli-Responsive Porous Polymer Membranes for Bioapplications

Electrically Switchable Polymer Brushes for Protein Capture and Release in Biological Environments**

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

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