Preponderance of Multidrug-resistant, Toxigenic, and Thermotolerant Enteropathogenic Bacteria in Raw and Cooked Seafood of Indo-Gangetic Basin and Associated Health Risks

Saha, Suvajit; Halder, Madhumanti; Mookerjee, Subham; Palit, A.

doi:10.1080/10498850.2020.1813858

Cited by 8 publications

(3 citation statements)

References 32 publications

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“…Lujiwa et al revealed that from 2008-2018, the top 15 major producers in aquaculture were using 67 different antibiotics, including previously banned antibiotics such as chloramphenicol, rifamycin, and penicillin [130] . This accounts for more than 25% of the available antibiotics, an alarming statistic given the increasing prevalence of MDR pathogens, including V. parahaemolyticus [146][147][148][149] . The outcomes of this study also showed that MDR V. parahaemolyticus is plentiful in seafood, where 81% of the isolates were resistant to more than one antibiotic, giving them an MAR index greater than 0.02.…”

Section: Discussionmentioning

confidence: 99%

Detection of multidrug resistant Vibrio parahaemolyticus and anti-Vibrio Streptomyces sp. MUM 178J

Loo,

Tan,

Law

et al. 2023

Prog Micobes Mol Biol

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The growing global population has increased the demand for seafood, making the aquaculture industry a vital food source. However, climate change has negatively impacted the industry as natural inhabitants of aquatic environments such as Vibrio parahaemolyticus are thriving in the warming waters. These foodborne pathogens can cause disease in marine animals, resulting in lowered production rates and substantial economic losses. V. parahaemolyticus is transmitted from these marine animals in aquaculture to humans via raw or undercooked seafood, resulting in gastroenteritis outbreaks. In addition, the extensive use of antibiotics in aquaculture to treat infections within marine animals has led to the development of multidrug-resistant (MDR) V. parahaemolyticus, which threatens the effectiveness of antimicrobial treatments. Thus, it is vital to explore the alternatives of antibiotics in aquaculture to manage the spread of antibiotic resistance. This study aimed to investigate the prevalence of MDR V. parahaemolyticus to provide insight into the current antibiotic resistance patterns of this pathogen and to determine potential anti-Vibrio properties of Streptomyces MUM 178J which was previously isolated from the soils of a mangrove forest in Sarawak, Malaysia. Colony morphology and toxR-assay indicated that all samples tested positive for V. parahaemolyticus. Further identification with genomic analyses confirmed that 64% (64/100) of the isolates were V. parahaemolyticus. The multiple antibiotic resistance index (MAR) of the isolates ranged between 0.07 and 0.57, with 81% (52/64) of the isolates resistant to more than one antibiotic. The isolates were most resistant to ampicillin (98.4%), followed by ceftazidime (53.1%). In contrast, the isolates were highly susceptible to nalidixic acid (98.4%), tetracycline (93.8%), and sulfamethoxazole/trimethoprim (90.6%). They also demonstrated susceptibilities of 89.1% to levofloxacin, oxytetracycline, and gentamicin. Streptomyces sp. MUM 178J exhibited antagonistic effects on select V. parahaemolyticus strain, RP0132, with a minimum inhibitory concentration of 12.5 mg/mL and a minimum bactericidal concentration of 50 mg/mL.

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Section: Discussionmentioning

confidence: 99%

Detection of multidrug resistant Vibrio parahaemolyticus and anti-Vibrio Streptomyces sp. MUM 178J

Loo,

Tan,

Law

et al. 2023

Prog Micobes Mol Biol

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“…b-lactams and tetracycline are commonly used in animals as feed additives or veterinary drugs to promote growth and prevent disease (Zhao et al, 2009). For example, extensive intrinsic resistance to doxycycline and neopenicillin has been reported (Saha et al, 2020). This may be related to the use of biofertilizers, which contain high levels of residual antibiotic compounds, resistance factors, and drugresistant bacteria (Aedo et al, 2014).…”

Section: Figurementioning

confidence: 99%

Pathogenicity and antibiotic resistance analysis of Vibrio species found in coastal water at mainly beach of Shenzhen, China

Huang

Anokyewaa²,

Wang³

et al. 2022

Front. Mar. Sci.

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Offshore industries and resources are the links between humans and the marine ecosystem. Several risk factors are associated with beach activities. The risk of Vibrio infection at bathing beaches is often overlooked as there are virtually no regulations regarding them. In this study, we investigated the current prevalence of pathogenic Vibrio spp. on the main beaches of Shenzhen. The risk indicator levels of pathogenic Vibrio species obtained were analyzed using 17 virulence genes mainly associated with Vibrio species and are responsible for several ailments and infections. A total of 60 Vibrio strains were isolated and identified by morphological observation, evolutionary tree alignment, and biochemical testing. There was a high abundance of Vibrio in the seawater. Also, a positive correlation was observed between the presence of virulence genes and the exhibition of high pathogenicity after artificially infecting fish with some of the virulent Vibrio species. In the infection experiment, it was observed that all the zebrafish infected with MEDF7 (Vibrio alginolyticus) and JSW-YELLOW (Vibrio harveyi) died a day after injection, with varying degrees of abdominal enlargement and skin ulceration. The mortality rates for strains with medium risk and low risk were 65% and 45%, respectively. Lethal dose 50 (LD50) values were 5.67 ×104 CFU/fish, 3.72 ×105 CFU/fish, and 4.31 ×105 CFU/fish for the high-risk, medium-risk, and low-risk strains of zebrafish, respectively. The results of the antibiotic sensitivity test showed that all the six experimental strains, except JSW-YELLOW, were resistant to doxycycline and neopenicillin. In summary, our study first identified and evaluated the pathogenicity of Vibrio in the Shenzhen beach baths, serving as a scientific benchmark for Vibrio risk prevention and control as well as guidance for Vibrio diagnosis through virulence factor detection and risk classification.

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“…Oysters are often consumed raw. However, raw oysters may contain many health-threatening factors including Vibrio cholera and enteropathogenic bacteria (28)(29)(30). Cooking can eliminate or reduce some harmful substances in oysters (31).…”

Section: Introductionmentioning

confidence: 99%

Bioaccessibility and Intestinal Transport of Deltamethrin in Pacific Oyster (Magallana Gigas) Using Simulated Digestion/NCM460 Cell Models

et al. 2021

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Deltamethrin (DEL) can be introduced into the food chain through bioaccumulation in Pacific oysters, and then potentially threaten human health. The objective of this study was to investigate the bioaccessibility of DEL in oysters with different cooking methods after simulated digestion. DEL content in different tissues of oysters going from high to low were gills, mantle, viscera, and adductor muscle. Bioaccessibility of DEL in oysters decreased after steaming (65%) or roasting (51%) treatments compared with raw oysters (82%), which indicated that roasting can be used as a recommended cooking method for oysters. In the simulated digestion process, the concentration of DEL in the digestive juice and the bioaccessibility of DEL were affected by the pH in the gastric phase. And the transport efficiency of DEL through the monolayer molecular membrane of NCM460 cells ranged from 35 to 45%. These results can help assess the potential harm to consumers of DEL in shellfish. Furthermore, it provides a reference for the impact of lipophilic toxins in seafood.

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Preponderance of Multidrug-resistant, Toxigenic, and Thermotolerant Enteropathogenic Bacteria in Raw and Cooked Seafood of Indo-Gangetic Basin and Associated Health Risks

Cited by 8 publications

References 32 publications

Detection of multidrug resistant Vibrio parahaemolyticus and anti-Vibrio Streptomyces sp. MUM 178J

Detection of multidrug resistant Vibrio parahaemolyticus and anti-Vibrio Streptomyces sp. MUM 178J

Pathogenicity and antibiotic resistance analysis of Vibrio species found in coastal water at mainly beach of Shenzhen, China

Bioaccessibility and Intestinal Transport of Deltamethrin in Pacific Oyster (Magallana Gigas) Using Simulated Digestion/NCM460 Cell Models

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