Preparing Thin Cross Sections of
            <i>Arabidopsis</i>
            Roots Without Embedding

Sotta, Naoyuki; Fujiwara, Toru

doi:10.2144/000114621

Cited by 8 publications

(2 citation statements)

References 9 publications

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“…(a-d) Crosssections of wheat roots, all at the same magnification. Inset of (c), Arabidopsis primary root cross-section, shown at same magnification as wheat second-order branch root (bar, 50 lm; from Sotta & Fujiwara, 2017). (e) Speculation about importance of root hairs as an epidermal barrier to Na + movement into the root.…”

Section: Importance Of Root System Anatomical Differences: Estimatingmentioning

confidence: 99%

Energy costs of salt tolerance in crop plants

et al. 2019

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Agricultureisexpandingintoregionsthatareaffectedbysalinity.Thisreviewconsiderstheenergetic costs of salinity tolerance in crop plants and provides a framework for a quantitative assessment of costs. Different sources of energy, and modifications of root system architecture that would maximize water vs ion uptake are addressed. Energy requirements for transport of salt (NaCl) to leaf vacuoles for osmotic adjustment could be small if there are no substantial leaks back across plasma membrane and tonoplast in root and leaf. The coupling ratio of the H +-ATPase also is a critical component. One proposed leak, that of Na + influx across the plasma membrane through certain aquaporin channels, might be coupled to water flow, thus conserving energy. For the tonoplast, control of two types of cation channels is required for energy efficiency. Transporters controlling the Na + and Cl À concentrations in mitochondria and chloroplasts are largely unknown and could be a major energy cost. The complexity of the system will require a sophisticated modelling approach to identify critical transporters, apoplastic barriers and root structures. This modelling approach will informexperimentationandallowaquantitativeassessmentoftheenergycostsofNaCltoleranceto guide breeding and engineering of molecular components.

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Section: Importance Of Root System Anatomical Differences: Estimatingmentioning

confidence: 99%

Energy costs of salt tolerance in crop plants

et al. 2019

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“…Arabidopsis seeds were surface sterilized by 5 min-immersion with 70% (v/v) ethanol, followed by 1-min immersion with 99.5% ethanol (Sotta and Fujiwara, 2017). After air-drying, the sterilized seeds resuspended with 0.1% (w/v) agar were sown on agar plates (seven seeds of each genotype per plate for sensitivity assay, and 36 seeds of a single genotype per plate for ionome assay).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Selection of Agar Reagents for Medium Solidification Is a Critical Factor for Metal(loid) Sensitivity and Ionomic Profiles of Arabidopsis thaliana

et al. 2020

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For researchers in the plant metal field, the agar reagent used for the solid plate medium is a problematic factor because application of different agar types and even a different lot of the same agar type can mask the plant metal-related phenotypes and impair the reproducibility. In this study, we systematically assessed effects of different agar reagents on metal(loid) sensitivity and element accumulation of the Arabidopsis metal sensitive mutants. Three established mutants (cad1-3, cad1-6, and abcc1/2), and three different types of purified agar reagents (Type A, Type E, and Nacalai) with two independent batches for each reagent were subjected to the analyses. First, we found that element concentrations in the agar reagents largely varied among the agar types. Then the effects of agar reagents on the mutant metal(loid)-sensitivity were examined under As(III), Hg(II), Cd(II), and excess Zn(II) conditions. A significant variation of the mutant metal(loid)-sensitivity was observed among the different agar plates but the variation depended on the combination of metal(loid) stress and agar reagents. Briefly, the typedependent variation was more evident under As(III) and Hg(II) than Cd(II) or excess Zn(II) conditions. A lot-dependent variation was also observed for Type A and Type E but not for Nacalai: hypersensitive phenotypes of cad1-3, cad1-6, and abcc1/2 under As(III) or Hg(II) treatments were diminished when different batches of the Type A or Type E agar types were used. We also found a significant variation of As and Hg accumulation in the wild-type and cad1-3. Plant As and Hg concentrations were remarkably higher and the difference between the genotypes was more evident when grown with Type A agar plates. We finally analyzed ionomic profiles in the plants exposed to As(III) stress. Agartype specific ionomic changes in cad1-3 were more observed with the Type A plates than with the Nacalai plates. The presented results overall suggest that suitability of agar reagents for metal(loid)-related phenotyping depends on the experimental design, and an inappropriate selection of agar reagents can mask even very clear phenotypes of the established mutant like cad1-3. We also discuss perspectives on the agar problem in the plant metal study.

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Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis

Yoshinari

Takano

2020

Methods in Molecular Biology

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Preparing Thin Cross Sections of Arabidopsis Roots Without Embedding

Cited by 8 publications

References 9 publications

Energy costs of salt tolerance in crop plants

Energy costs of salt tolerance in crop plants

Selection of Agar Reagents for Medium Solidification Is a Critical Factor for Metal(loid) Sensitivity and Ionomic Profiles of Arabidopsis thaliana

Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis

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