Preparing Adipogenic Hydrogel with Neo-Mechanical Isolated Adipose-Derived Extracellular Vesicles for Adipose Tissue Engineering

Nie, Jiaying; Zhu, Yuanzheng; Wang, Jiang-Wen; Hu, Xuan; Wang, Zhaohui; Wu, Shu; Yi, Yangyan

doi:10.1097/prs.0000000000008186

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…4,18 Also, it should not elicit an inflammatory or immune response and be well tolerated by the recipient organism. 22,[31][32][33] Other qualities may also be desirable in the context of human application, such as relatively short production times, minimal surgical intervention, and a low complication rate. Unfortunately, no perfect method exists yet.…”

Section: Discussionmentioning

confidence: 99%

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Techniques and Innovations in Flap Engineering: A Review

Kouniavski

Egozi

Wolf

2022

Plastic and Reconstructive Surgery - Global Open

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Background: Currently, the gold standard for complex defect reconstruction is autologous tissue flaps, with vascularized composite allografts as its highest level. Good clinical results are obtained despite considerable obstacles, such as limited donor sites, donor site morbidity, and complex operations. Researchers in the field of tissue engineering are trying to generate novel tissue flaps requiring small or no donor site sacrifice. At the base of existing technologies is the tissue’s potential for regeneration and neovascularization. Methods: A review was conducted identifying relevant published articles in PubMed on the subject of flap engineering, with the focus on plastic surgery. This review article surveys contemporary technologies in flap engineering, including cell sheet technology, prefabricated flaps, and tissue engineering chambers. Conclusions: Some of the described procedures, though not yet ready for clinical use, are certainly ready for trial in large animal models and even human studies. Tissue engineering is a promising field for the handling of large and complex tissue defects.

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Section: Discussionmentioning

confidence: 99%

“…An attempt to perfect the hydrogel technique was made by Nie et al 31 They mixed decellularized adipose tissue hydrogel with adipose tissue-derived extracellular vesicles and injected them subcutaneously in an animal model. The addition of adipose vesicles with their rich biological content improved angiogenesis and adipogenesis.…”

Section: Hydrogelsmentioning

confidence: 99%

Techniques and Innovations in Flap Engineering: A Review

Kouniavski

Egozi

Wolf

2022

Plastic and Reconstructive Surgery - Global Open

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“…In addition to EVs derived from MSC, AT-EVs [345][346][347] and lipoma tissue EVs [348] were studied.…”

Section: Extracellular Vesiclesmentioning

confidence: 99%

Vascularized adipose tissue engineering: moving towards soft tissue reconstruction

Peirsman,

Nguyen,

Van Waeyenberge

et al. 2023

Biofabrication

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Soft tissue defects are a common clinical challenge mostly caused by trauma, congenital anomalies and oncological surgery. Current soft tissue reconstruction (STR) options include synthetic materials (fillers and implants) and autologous adipose tissue transplantation through flap surgery and/or lipotransfer. Both reconstructive options hold important disadvantages to which vascularized adipose tissue engineering (VATE) strategies could offer solutions. In this review, we first summarized pivotal characteristics of functional adipose tissue (FAT) such as the structure, function, cell types, development and extracellular matrix (ECM). Next, we discussed relevant cell sources and how they are applied in different state-of-the-art VATE techniques. Herein, biomaterial scaffolds and hydrogels, ECMs, spheroids, organoids, cell sheets, 3D bioprinting and microfluidics are overviewed. Also, we included extracellular vesicles and emphasized their potential role in VATE. Lastly, current challenges and future perspectives in VATE are pointed out to help to pave the road towards clinical applications.

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“…We read with great interest the article published by Nie et al titled “Preparing Adipogenic Hydrogel with Neo-Mechanical Isolated Adipose-Derived Extracellular Vesicles for Adipose Tissue Engineering.” 1 We sincerely congratulate the authors on their contributions to the study of hydrogel and extracellular vesicles. Although the data presented by Nie and colleagues are encouraging, we still have some ideas and perspectives that we would like to share.…”

mentioning

confidence: 99%

Preparing Adipogenic Hydrogel with Neo-Mechanical Isolated Adipose-Derived Extracellular Vesicles for Adipose Tissue Engineering

Yan¹,

Hu²,

Wang³

2022

Plastic &Amp; Reconstructive Surgery

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W e read with great interest the article published by Nie et al. titled "Preparing Adipogenic Hydrogel with Neo-Mechanical Isolated Adipose-Derived Extracellular Vesicles for Adipose Tissue Engineering." 1 We sincerely congratulate the authors on their contributions to the study of hydrogel and extracellular vesicles. Although the data presented by Nie and colleagues are encouraging, we still have some ideas and perspectives that we would like to share. So far, there indeed have been various studies on clinical treatments using extracellular vesicles and hydrogel. This has been a hot topic in recent years, and the conclusions have been positive. There are still some points we would like to discuss with the authors. First, in the animal model, 0.2 ml of adipose tissue-derived extracellular vesicle (ATEV)-decellularized adipose tissue hydrogel was injected subcutaneously into the right backs of mice, while the same volume of decellularized adipose tissue hydrogel was injected into the left backs; however, results showed there was no statistical difference between the two groups in adipose tissue volume retention. We would like to know more about the ATEVs' biological activity after decellularized adipose tissue hydrogel injection. It may be more convincing if the authors added a step to verify that the biological activity of the ATEVs did not change before the next injection. Second, hydrogel is a hot topic, and its biological functions have been widely studied. Many novel bioactive hydrogels have been reported, with some having the function of inducing adipose-derived stem cells into osteoblasts and some able to stimulate proliferation by imitating the extracellular matrix environment. 2 If most ATEVs survive, it shows that decellularized adipose tissue hydrogel possesses biological activities, such as promoting adipogenesis, stimulating ATEV proliferation, or something else. Further study of the biological mechanism may be needed. Finally, there are internationally recognized methods for the extraction of extracellular vesicles that have been approved by the International Vesicle Association, such as ultracentrifugation techniques, polymer precipitation, size-based isolation techniques, immunoaffinity chromatography, ultrafiltration, and the microfluidic technique. 3 We wonder, is the authors' method better at extracting quantity compared with these methods? A more efficient method is indeed needed at present.In conclusion, while the authors' neo-mechanical method for isolating ATEVs and preparing adipogenic hydrogel proved to be enlightening, more improvements and developments are necessary to lend this method credibility, reliability, and high-efficiency, either as a production machine to create business value or as a ready-off-the-shelf therapy for clinical treatments. This experience might be more complete if further research is conducted by the authors to identify specific pathways that promote adipogenesis and angiogenesis.

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Preparing Adipogenic Hydrogel with Neo-Mechanical Isolated Adipose-Derived Extracellular Vesicles for Adipose Tissue Engineering

Cited by 7 publications

References 34 publications

Techniques and Innovations in Flap Engineering: A Review

Techniques and Innovations in Flap Engineering: A Review

Vascularized adipose tissue engineering: moving towards soft tissue reconstruction

Preparing Adipogenic Hydrogel with Neo-Mechanical Isolated Adipose-Derived Extracellular Vesicles for Adipose Tissue Engineering

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