Preparative separation of quaternary ammonium alkaloids from Coptis chinensis Franch by pH-zone-refining counter-current chromatography

Li, Jia; Wang, Xiao; Duan, Wenjuan; Zhang, Tianyou; Ito, Yoichiro

doi:10.1016/j.chroma.2014.10.043

Cited by 35 publications

(22 citation statements)

References 22 publications

(18 reference statements)

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“…In general, it has often been difficult to isolate alkaloids from CR. However, through the application of suitable solvent systems in high-speed countercurrent chromatography, such as n -hexane–ethyl-acetate–methanol–water (2:5:2:5) [ 13 ] and chloroform–methanol–water (4:3:3, v/v) with HCl (60 mM) and triethylamine (5 mM) added into the upper aqueous phase and the lower organic phase [ 14 ], the highly pure main alkaloids could be readily separated and obtained in considerable yields. Spectroscopic analyses were employed in structural determination.…”

Section: Phytochemical Investigationmentioning

confidence: 99%

Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity

et al. 2018

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BackgroundCoptidis rhizoma (CR) is the dried rhizome of Coptis chinensis Franch., C. deltoidea C. Y. Cheng et Hsiao or C. teeta Wall. (Ranunculaceae) and is commonly used in Traditional Chinese Medicine for the treatment of various diseases including bacillary dysentery, typhoid, tuberculosis, epidemic cerebrospinal meningitis, empyrosis, pertussis, and other illnesses.MethodsA literature survey was conducted via SciFinder, ScieneDirect, PubMed, Springer, and Wiley databases. A total of 139 selected references were classified on the basis of their research scopes, including chemical investigation, quality evaluation and pharmacological studies.ResultsMany types of secondary metabolites including alkaloids, lignans, phenylpropanoids, flavonoids, phenolic compounds, saccharides, and steroids have been isolated from CR. Among them, protoberberine-type alkaloids, such as berberine, palmatine, coptisine, epiberberine, jatrorrhizine, columamine, are the main components of CR. Quantitative determination of these alkaloids is a very important aspect in the quality evaluation of CR. In recent years, with the advances in isolation and detection technologies, many new instruments and methods have been developed for the quantitative and qualitative analysis of the main alkaloids from CR. The quality control of CR has provided safety for pharmacological applications. These quality evaluation methods are also frequently employed to screen the active components from CR. Various investigations have shown that CR and its main alkaloids exhibited many powerful pharmacological effects including anti-inflammatory, anti-cancer, anti-diabetic, neuroprotective, cardioprotective, hypoglycemic, anti-Alzheimer and hepatoprotective activities.ConclusionThis review summarizes the recent phytochemical investigations, quality evaluation methods, the biological studies focusing on CR as well as its main alkaloids.

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Section: Phytochemical Investigationmentioning

confidence: 99%

Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity

et al. 2018

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“…The specific primer sequences were synthesized in Aoke Corporation (Shanghai, China). Berberine (reference standards, with purity of over 98%) (Sun et al, ) was obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Berberine Inhibits Adipogenesis in Porcine Adipocytes via AMP‐Activated Protein Kinase‐Dependent and ‐Independent Mechanisms

Yang

Liu

et al. 2019

Lipids

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Excessive adipogenesis in adipocytes results in obesity. Berberine, a natural isoquinoline alkaloid, has antiobesity properties. However, the underlying molecular mechanisms have remained unclear up to now. In this study, porcine adipocytes were cultured and treated with berberine. Cellular lipid content was measured by Oil Red O staining extraction. The role of an adenosine monophosphate‐activated protein kinase (AMPK) signaling pathway was evaluated by the phosphorylation detection of AMPKα protein and knockdown of AMPK alpha1 (Ampka1) gene. Gene expressions were analyzed by Western blot and real‐time reverse transcription‐polymerase chain reaction (RT‐PCR). The results showed that berberine reduced lipid accumulation in porcine adipocytes in a dose‐ and time‐dependent manner and increased phosphorylation of AMPKα. Furthermore, berberine significantly downregulated the mRNA expression of related genes to adipogenesis including peroxisome proliferator activated receptor gamma 2 (Pparg2), CCAAT/enhancer‐binding protein alpha (Cebpa), Cebp beta (Cebpb), sterol regulatory element binding transcription factor 1 (Srebf1), acetyl‐CoA carboxylase‐1 (Acc‐1), fatty acid synthase (Fas), fatty acid binding protein 4 (Fabp4), and stearoyl‐CoA desaturase 1 (Scd1). Knockdown of Ampka1 markedly reversed the inhibitory effect of berberine on lipid accumulation and mRNA expression of the above genes except Cebpb in porcine adipocytes. Meanwhile, the protein expression of these adipogenic genes in response to berberine and Ampka1 knockdown paralleled the alterations of their mRNA level. These results suggest that berberine inhibits adipogenesis in porcine adipocytes via AMPK‐dependent and ‐independent multiple mechanisms, which would provide an important idea for the reduction of porcine body fat, as well as the prevention and treatment of human obesity.

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“…The concentration of the eluter in the mobile phase and retainer in the stationary phase will influence the concentration and retention time of analyte as well as the retention rate of the stationary phase [22]. Usually, equimolar concentrations of retainer and eluter such as 10-20 mM were first taken into account, which produced a satisfactory separation in most cases [14].…”

Section: Selection Of Two Phase Solvent Systemmentioning

confidence: 99%

An efficient and target-oriented sample enrichment method for preparative separation of minor alkaloids by pH-zone-refining counter-current chromatography

Ren

Hou

Zhang

et al. 2015

Journal of Chromatography A

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Preparative separation of quaternary ammonium alkaloids from Coptis chinensis Franch by pH-zone-refining counter-current chromatography

Cited by 35 publications

References 22 publications

Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity

Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity

Berberine Inhibits Adipogenesis in Porcine Adipocytes via AMP‐Activated Protein Kinase‐Dependent and ‐Independent Mechanisms

An efficient and target-oriented sample enrichment method for preparative separation of minor alkaloids by pH-zone-refining counter-current chromatography

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