Preparative separation and purification of Cyclosporin D from fungus Hypoxylon Spp. by improved closed-loop recycling counter-current chromatography

Li, Hepeng; Zhang, Feiying; Jin, Qun-Ying; Zhu, Tong

doi:10.1016/j.chroma.2021.462221

Cited by 18 publications

(3 citation statements)

References 21 publications

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“…Additionally, HSCCC displays numerous merits compared with conventional liquid-liquid extraction and solid-phase chromatography methods, such as no irreversible adsorption, great capacity, great injected sample recovery, low solvent consumption, and low sample denaturation risk [18]. Currently, various modes, including normal phase [19], reverse phase [20], dual mode [21], pH zone refining [22], gradient mode [23], as well as recycling mode [20,[24][25][26], are available. Therefore, HSCCC is extensively applied in isolating and purifying effective ingredients from natural products.…”

Section: Introductionmentioning

confidence: 99%

Application of one‐step inner‐recycling counter‐current chromatography for the preparative separation and purification of chemical constituents from the rhizome of Bergenia ciliate (haw.) Sternb

Aziz,

Akhter,

Hussain

et al. 2023

J of Separation Science

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Bergenia ciliata (haw.) Sternb, the renowned pharmaceutical plant in Jammu and Kashmir of Pakistan, is widely applied in treating different illnesses including diabetes, diarrhea, and vomiting. This work employed an efficient one‐step inner‐recycling counter‐current chromatography for preparative separating and purifying compounds with similar partition coefficients from the rhizome of Bergenia ciliate (haw.). Five compounds, including quercetin rhamnodiglucoside (1), quercetin‐3‐O‐rutinoside (2), bergenine (3), kaempferol (4), and palmatic acid (5), were successfully separated using the optimized biphasic solvent system that contained ter‐butylmetylether/n‐butanol/acetonitrile/water (2:2:1:5, v/v) with the purities over 98%. Mass spectrometry and nuclear magnetic resonance were conducted for structural identification. As a result, our proposed strategy might be applied in separating compounds with similar partition coefficients, which was advantageous with regard to the less solvent and time consumption, and the increased number of theoretical plates.

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Section: Introductionmentioning

confidence: 99%

Application of one‐step inner‐recycling counter‐current chromatography for the preparative separation and purification of chemical constituents from the rhizome of Bergenia ciliate (haw.) Sternb

Aziz,

Akhter,

Hussain

et al. 2023

J of Separation Science

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show abstract

“…The application of chromatography principles to solvent extraction processes can greatly improve industrial separation technologies. In solvent extraction and in liquidliquid chromatography, referred to as "counter-current chromatography" (CCC) [12][13][14][15][16] and "centrifugal partition chromatography" (CPC) [17][18][19], the separation of substances is based on their different solubility in two liquid phases. Countercurrent chromatography differs from traditional liquid chromatography in that the so-called stationary phase in a CCC device is not fixed on a solid carrier but is kept mobile in the device due to centrifugal forces.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, CCC separations are mainly used for analytical and preparative purposes for the separation and purification of herbal pharmaceutical products [12][13][14][15][16][17][18][19]. CCC devices in the form of a tube wound in one or several layers around the drum of a planetary centrifuge (hydrodynamic devices), or in the form of a cascade of chambers on the surface of a cylinder, or on a pack of discs mounted on the shaft of a conventional centrifuge (hydrostatic devices), are difficult to scale up.…”

Section: Introductionmentioning

confidence: 99%

Separation of Rare Earth Elements in Multistage Extraction Columns in Chromatography Mode: Experimental Study and Mathematical Simulation

et al. 2023

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The application of liquid–liquid chromatography principles to solvent extraction processes in hydrometallurgy can greatly simplify rare earth metal separation technologies by separating multicomponent mixtures in one technological operation. In this study, the chromatographic separation of rare earth elements (REEs) in multistage extraction columns was experimentally studied under conditions of impulse sample injection—single and multiple loading of large volumes of metal salt solution into the installation. The results obtained showed the feasibility of operating sieve plate extraction columns in the liquid–liquid chromatography mode. A closed-loop recycling technology is proposed for the separation of rare earth elements in multistage extraction columns operating in the liquid–liquid chromatography mode. For further development and industrial implementation of this technology, experimental studies should be conducted on intensified multistage extraction columns, such as sectioned columns with agitators and vibrating plate columns. Computer simulation of the chromatographic separation of rare earth elements by closed-loop recycling liquid–liquid chromatography was carried out.

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Recycling Preparative Liquid Chromatography, the Overlooked Methodology for the Purification of Natural Products

Pereda-Miranda,

Castañeda-Gómez,

Fragoso-Serrano

2024

Rev. Bras. Farmacogn.

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Frequently, preparative high-performance liquid chromatography separations of complex natural product mixtures by adsorption chromatography are erratic to achieve full baseline separation. Purification of metabolites with similar or identical polarity, such as epimers, diastereoisomers, homologs in a series, and geometric or positional isomers, by a single chromatographic run, is not properly achieved. Consequently, recycling preparative high-performance liquid chromatography has been proposed with a closed-loop recycling valve designed to increase the capacity of separation of mixtures of low-resolution peaks by a series of consecutive passes through the same column. Thus, the sample zone is basically recycled back into the column to continue the separation process in a closed-loop system. A consequence of this recycling mode is the increment in the number of theoretical plates with each cycle and maintaining a minium peak dispersion in the resulting chromatogram with no additional solvent needed for recycling. Pure samples are collected when the baseline resolution is achieved. Therefore, compounds with comparable physicochemical characteristics are fully separated to provide pure single chemical entities suitable for structure elucidation and further biological assessments. This review article examines the essential fundaments of this efficient method and its advances in isolation of natural products that have made the laborious purification processes less demanding and less time-consuming. Several applications that show the purification of natural products from small to large bioactive natural molecules by recycling preparative high-performance liquid chromatography are revised. Graphical abstract

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Preparative separation and purification of Cyclosporin D from fungus Hypoxylon Spp. by improved closed-loop recycling counter-current chromatography

Cited by 18 publications

References 21 publications

Application of one‐step inner‐recycling counter‐current chromatography for the preparative separation and purification of chemical constituents from the rhizome of Bergenia ciliate (haw.) Sternb

Application of one‐step inner‐recycling counter‐current chromatography for the preparative separation and purification of chemical constituents from the rhizome of Bergenia ciliate (haw.) Sternb

Separation of Rare Earth Elements in Multistage Extraction Columns in Chromatography Mode: Experimental Study and Mathematical Simulation

Recycling Preparative Liquid Chromatography, the Overlooked Methodology for the Purification of Natural Products

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