Preparative procedures and purity assessment of collagen proteins

Deyl, Z.; Mikšı́k, Ivan; Eckhardt, Adam

doi:10.1016/s1570-0232(03)00158-2

Cited by 51 publications

(32 citation statements)

References 108 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For both species, salmon (a) and codfish (b) electrophoresis analysis demonstrated the presence of α 1 and α 2 chains, correspondent to type I collagen [1,46], as well the presence of β dimer. The β component indicates that both collagens contain inter-molecular crosslinks.…”

Section: Sds-page Analysismentioning

confidence: 99%

Cosmetic Potential of Marine Fish Skin Collagen

et al. 2017

View full text Add to dashboard Cite

Many cosmetic formulations have collagen as a major component because of its significant benefits as a natural humectant and moisturizer. This industry is constantly looking for innovative, sustainable, and truly efficacious products, so marine collagen based formulations are arising as promising alternatives. A solid description and characterization of this protein is fundamental to guarantee the highest quality of each batch. In the present study, we present an extensive characterization of marine-derived collagen extracted from salmon and codfish skins, targeting its inclusion as component in cosmetic formulations. Chemical and physical characterizations were performed using several techniques such as sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier Transformation Infrared (FTIR) spectroscopy rheology, circular dichroism, X-ray diffraction, humidity uptake, and a biological assessment of the extracts regarding their irritant potential. The results showed an isolation of type I collagen with high purity but with some structural and chemical differences between sources. Collagen demonstrated a good capacity to retain water, thus being suitable for dermal applications as a moisturizer. A topical exposure of collagen in a human reconstructed dermis, as well as the analysis of molecular markers for irritation and inflammation, exhibited no irritant potential. Thus, the isolation of collagen from fish skins for inclusion in dermocosmetic applications may constitute a sustainable and low-cost platform for the biotechnological valorization of fish by-products.

show abstract

Section: Sds-page Analysismentioning

confidence: 99%

Cosmetic Potential of Marine Fish Skin Collagen

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Collagen II was abstracted from bovine cartilage with salt precipitation method (Deyl et al, 2003) in the Institute of TCM and Pharmacology, Southern Medical University. Freund's complete and incomplete adjuvants were purchased from Sigma-Aldrich.…”

Section: Animals and Materialsmentioning

confidence: 99%

Anti-inflammatory effect of Sanshuibaihu decoction may be associated with nuclear factor-κB and p38 MAPKα in collagen-induced arthritis in rat

Yang¹,

Xiao²,

Wu³

et al. 2010

Journal of Ethnopharmacology

View full text Add to dashboard Cite

“…In order to identify individual collagen types (details see, e.g. [8]) the low molecular mass peptides are particularly important as some of them are specific for in- Fig. 3.…”

Section: Resultsmentioning

confidence: 99%