Preparative low-pressure chromatography of antibiotics on a column of diol-bonded silica gel

Rasmussen, Ronald R.; Scherr, Michael H.

doi:10.1016/s0021-9673(01)94609-6

Cited by 10 publications

(3 citation statements)

References 15 publications

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“…However, the peaks were not completely separated while the flow rate was too fast to have plenty of time for separation, causing lower separation efficiency. Generally speaking, the lower the flow rate is, the more possibly a good separation can be obtained, because of the separation time is enough for the sample diffusion and elution in column (Rasmussen & Scherr, ). Nevertheless, taking time consumption and separation efficiency into consideration, the optimum flow rate was 6 ml/min.…”

Section: Resultsmentioning

confidence: 99%

“…Generally speaking, the lower the flow rate is, the more possibly a good separation can be obtained, because of the separation time is enough for the sample diffusion and elution in column (Rasmussen & Scherr, 1987). Nevertheless, taking time consumption and separation efficiency into consideration, the optimum flow rate was 6 ml/min.…”

Section: Technological Optimization Of Preparative Low-pressure Colmentioning

confidence: 99%

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Purification, characterization, and identification of 3‐hydroxy‐4‐methoxy benzal acrolein–an intermediate of synthesizing advantame

Chen

Qiang

Wang

et al. 2020

Food Science & Nutrition

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Advantame is a novel sweetener, and 3‐hydroxy‐4‐methoxy benzal acrolein is an important intermediate to synthesize the sweetener. The aim of this study was to evaluate a new low‐cost method to purify 3‐hydroxy‐4‐methoxy benzal acrolein, and the crude intermediate was used as raw material. The intermediate was purified using a low‐pressure column chromatography with a C18 column, using a methanol‐water (6:4, v/v) at a flow rate of 6.0 ml/min, and the loading amount of the crude intermediate in solution was 10.0 mg in total. A method for the analysis of 3‐hydroxy‐4‐methoxy benzal acrolein was established using high‐performance liquid chromatography (HPLC). This method was validated in terms of its linearity, repeatability, accuracy, detection limit, and quantitation limit. The calibration curves of 3‐hydroxy‐4‐methoxy benzal acrolein were linear (r > .999) over a wide concentration range of 0.005–0.08 mg/ml, by comparing the ratio of signal to noise, and the detection limit was 5.0 ng/ml and the quantification limit was 15.0 ng/ml. Good repeatability was obtained (RSD < 2%, n = 6), and the recoveries calculated using mixed sample previously quantified ranged from 94.5% to 106.37%. As long as, this method has been successfully applied to the analysis of 3‐hydroxy‐4‐methoxy benzal acrolein; therefore, the method can be put into practical use during the industrial synthesis and real‐time detection of the intermediate.

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Section: Resultsmentioning

confidence: 99%

Section: Technological Optimization Of Preparative Low-pressure Colmentioning

confidence: 99%

Purification, characterization, and identification of 3‐hydroxy‐4‐methoxy benzal acrolein–an intermediate of synthesizing advantame

Chen

Qiang

Wang

et al. 2020

Food Science & Nutrition

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“…An attempt to purify the crude extract of Lyngbya by batch elution over Si gel recovered only 4% of the PDBu activity. To circumvent this acid/silica instability, we used diol phase-bonded material, which has been suggested as a substitute for Sephadex LH-20 (11), used previously in the isolation of debromoaphysiatoxin (3). By eluting with nonacidic organic solvents we concentrated bioactivity and obtained high mass recovery, with a similar polarity profile to the silica elution.…”

Section: Resultsmentioning

confidence: 99%

Dereplication of Phorbol Bioactives: Lyngbya majuscula and Croton cuneatus

et al. 1990

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Lyngbya majuscula and Croton cuneatus were used as prototypes for the dereplication of phorbol ester receptor binding activity using a combination of hplc-uv and online phorbol dibutyrate (PDBu) receptor binding and batch fractionation over either Si gel or diolbonded Si gel. Debromoaplysiatoxin was responsible for the bioactivity of Lyngbya, whereas a complex of potent phorbol esters was detected in C. cuneatus.

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Isolation and Purification of Secondary Metabolites

McAlpine

Hochlowski

1994

Discovery of Novel Natural Products With Therapeutic Potential

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Preparative low-pressure chromatography of antibiotics on a column of diol-bonded silica gel

Cited by 10 publications

References 15 publications

Purification, characterization, and identification of 3‐hydroxy‐4‐methoxy benzal acrolein–an intermediate of synthesizing advantame

Purification, characterization, and identification of 3‐hydroxy‐4‐methoxy benzal acrolein–an intermediate of synthesizing advantame

Dereplication of Phorbol Bioactives: Lyngbya majuscula and Croton cuneatus

Isolation and Purification of Secondary Metabolites

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