Preparative isolation of osthol and xanthotoxol from Common Cnidium Fruit (Chinese traditional herb) using stepwise elution by high-speed counter-current chromatography

Simultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plantCnidium monnieri by high-speed counter-current chromatography

“…The composition of the crude extract was similar to that described by Feng et al [23], and xanthotoxol was a minor component. However, osthol and xanthotoxol were reported to be the main components of the crude extract by Wei et al [21]. The reason could be that the extraction procedure in the present paper was similar to that used by Feng et al [23], but different from that of Wei et al [21], details of which remain unknown because the crude extract was obtained from a factory.…”

“…The reason could be that the extraction procedure in the present paper was similar to that used by Feng et al [23], but different from that of Wei et al [21], details of which remain unknown because the crude extract was obtained from a factory. Therefore, it is not surprising that four out of the five coumarins separated and purified in the present study are different from those reported by Wei et al [21].…”

“…The advantage of a liquid stationary phase is that the solutes have access to the whole volume of the phase, leading to a high efficiency of extraction, and HSCCC is fundamentally a preparative separation technique [11], which has been used for the preparative isolation and purification of several natural products [12 -20]. Although HSCCC was recently proposed for the separation and purification of coumarins from the herbal medicine Cnidium monnieri (L.) Cusson [21], the number and amounts of coumarins purified, however, were both small. The aim of this study, therefore, was to develop an efficient HSCCC method for the preparative separation and purification of five coumarins simultaneously from the medicinal plant Cnidium monnieri (L.) Cusson in larger amounts.…”

Design, Synthesis and Antifungal Activity of Coumarin Ring-Opening Derivatives

“…As the structural core, coumarin occurs widely in natural products, drugs and agrochemicals (Figure 1), these important applications have generated considerable interest in this ring system and various fused coumarin derivatives have been reported [3][4][5][6]: Osthole, a natural O-methylated coumarin found in Cnidium Monnieri, a traditional Chinese herbal medicine that has been used as as a fungicidein China for a long history,and shows antifungal activity against Rhizoctonia solani and a broad spectrum of other phytopathogenic fungi [7][8][9]; Warfarin and Acenocoumarol are anticoagulants normally used in the prevention of thrombosis and thromboembolism, function as the vitamin K antagonists [10][11][12]; and Coumoxystrobin (SYP-3375) is a coumarin-containing strobilurin fungicide that contains an (E)-methyl 3-methoxy-2-phenylacrylate substructure and displays a broad spectrum of antifungal activity [13][14][15]. In our previous work, Osthole was used as the lead structure to carry out structural optimization, and some synthesized furan [3,2-c]coumarin derivatives showed potent antifungal activity [16][17][18].…”

Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography

“…High-speed counter-current chromatography (HSCCC) is a unique liquid-liquid partition chromatography, which has been applied to the separation of a number of natural products [8][9][10][11][12][13][14][15][16]. However, no report has been published on the use of HSCCC for the isolation and purification of the highly bioactive zeaxanthin, in particular, from microalgae.…”