Preparative isolation and analysis of alcohol dehydrogenase inhibitors from <i>Glycyrrhiza uralensis</i> root using ultrafiltration combined with high‐performance liquid chromatography and high‐speed countercurrent chromatography

Chen, Miao; Liu, Liangliang; Chen, Xiaoqing

doi:10.1002/jssc.201400051

Cited by 28 publications

(19 citation statements)

References 29 publications

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“…As the holes of the membrane were smaller than xanthine oxidase molecules, the enzyme was retained by membrane together with binding ligands after ultrafiltration. Through the analysis of the filtrate, the peaks with reduced peak areas could be considered as ligands (potential inhibitors) [20]. The chromatogram of the filtrate after ultrafiltration is shown in Figure 1b, the peak areas of four main peaks marked with numbers were reduced obviously.…”

Section: Screening Of Xanthine Oxidase Inhibitors and Antioxidantsmentioning

confidence: 99%

Xanthine Oxidase Inhibitors Screening, Antioxidation, and DNA Protection Properties of Geranium wilfordii Maxim

Liu

Mei

Xiao

et al. 2019

eFood

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KeywordsAntioxidation DNA damage DPPH spiking inhibitor ultrafiltration xanthine oxidase A B S T R A C TGeranium wilfordii Maxim is a commonly used traditional medicine and as decoction pieces in drinks for treating acute and chronic rheumatalgia. Earlier research showed it could inhibit xanthine oxidase and had a strong antioxidative activity. In this study, four compounds namely, corilagin, geraniin, ellagic acid, and myricetrin were screened and identified as xanthine oxidase inhibitors and antioxidants using ultrafiltration combined liquid chromatography-mass spectrometry (LC-MS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) spiking combined LC-MS methods. Ellagic acid showed the greatest inhibition with IC 50 of 0.018 ± 0.001 mM better than that of allopurinol. The kinetic parameters exhibited the mode of xanthine oxidase inhibitions by corilagin, geraniin, and myricetrin were the competitive types, whereas the ellagic acid was found as an uncompetitive inhibitor. In vitro DNA nicking assay showed these four compounds protected DNA from reactive oxygen species. Correlation analysis and partial least squares analysis showed there was a moderate correlation between ellagic acid and DPPH˙ scavenging activity. Our findings revealed four compounds in G. wilfordii Maxim contributed satisfied radicals scavenging, xanthine oxidase inhibition, and DNA damage protective activities. More comprehensive research on pharmaceutical applications and phytochemical profile of G. wilfordii Maxim could be further studied.

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Section: Screening Of Xanthine Oxidase Inhibitors and Antioxidantsmentioning

confidence: 99%

Xanthine Oxidase Inhibitors Screening, Antioxidation, and DNA Protection Properties of Geranium wilfordii Maxim

Liu

Mei

Xiao

et al. 2019

eFood

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“…In the research on screening with ultrafiltration, binding degree was often used as an index to evaluate the performance of screening system and provide useful information for determining the strength of inhibitory activity. 28,29 In this study, binding degrees were used to evaluate the binding performances of ten flavonoids. As shown in Table 2, the binding degree of apigenin was the highest (94.1%).…”

Section: Alpha-amylase Inhibitors Screening From Flavonoidsmentioning

confidence: 99%

Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

Liao¹,

Chen²,

Liu³

et al. 2017

J. Braz. Chem. Soc.

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Lotus leaf is gaining growing popularity due to its various benefits and widely usage. In this paper, ten flavonoids in lotus leaf extract were analyzed by high performance liquid chromatography (HPLC). Centrifugal ultrafiltration combined liquid chromatography was used to screen alphaamylase inhibitors from ten flavonoids mixture and the binding degrees ranged from 2.34 to 94.1%. The alpha-amylase inhibition and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) antioxidant activity of ten flavonoids were verified as well. Apigenin, kaempferol and isorhamnetin with relatively higher binding degrees showed higher inhibition on alpha-amylase as well. The interactions between these flavonoids and alpha-amylase were investigated by spectroscopic method. As a result, the fluorescence quenching could be considered as static quenching because the quenching rate constant (K q ) values were higher than 2.0 × 10 10 L mol -1 s -1. The binding constants (log 10 K a ) of ten flavonoids were in the range of 5.971 to 7.417 L mol -1 . The hydrogenation of C 2 =C 3 double bond of apigenin and quercetin decreased the affinity for alpha-amylase (1.92-and 2.82-fold). The hydroxylation on 3 and 3' position decreased the affinity for alpha-amylase. Moreover, the glycosylation with different sugar moiety improved in varying degrees of affinity. The hydrogen bond force might be important in the binding between alpha-amylase and flavonoids.

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“…To overcome these limitations and enhance the throughput of drug discovery, molecular methods based on high-affinity and high-selectivity protein-ligand interactions have been successfully used [12]. Among them, combined ultrafiltrationliquid chromatography-mass spectrometry (UF-LC-MS) is a powerful method for screening biologically active compounds from botanical extracts, because the UF step facilitates the separation of ligand-receptor complexes from unbound compounds and the subsequent LC-MS step is used to identify the ligands [13,14].…”

Section: Introductionmentioning

confidence: 99%

Extraction and isolation of acetylcholinesterase inhibitors from Citrus limon peel using an in vitro method

Liu

Hou

et al. 2020

J of Separation Science

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A simple and efficient ultrafiltration-liquid chromatography-mass spectrometrybased method was developed for the rapid screening and identification of ligands from Citrus limon peel, which are suitable acetylcholinesterase inhibitors. Subsequently, the anti-Alzheimer's activity of these compounds was assessed using a PC12 cell model. Six major compounds, viz. neoeriocitrin, isonaringin, naringin, hesperidin, neohesperidin, and limonin, were identified as potent acetylcholinesterase inhibitors.A continuous and efficient online method, which involved the use of a microwaveassisted extraction device, solvent concentration tank, and centrifugal partition chromatography column, was developed for the scale-up of these compounds, and the obtained compounds presented high purity. Next, their bioactivity was evaluated using a PC12 cell model. This novel approach, which was based on ultrafiltration-liquid chromatography-mass spectrometry, microwave-assisted extraction online coupled with solvent concentration tank, and centrifugal partition chromatography along with in vitro evaluation, could represent a powerful tool for the screening and extraction of acetylcholinesterase inhibitors from complex matrices, and could be a useful platform for the large-scale production of bioactive and nutraceutical ingredients.

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Preparative isolation and analysis of alcohol dehydrogenase inhibitors from Glycyrrhiza uralensis root using ultrafiltration combined with high‐performance liquid chromatography and high‐speed countercurrent chromatography

Cited by 28 publications

References 29 publications

Xanthine Oxidase Inhibitors Screening, Antioxidation, and DNA Protection Properties of Geranium wilfordii Maxim

Xanthine Oxidase Inhibitors Screening, Antioxidation, and DNA Protection Properties of Geranium wilfordii Maxim

Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

Extraction and isolation of acetylcholinesterase inhibitors from Citrus limon peel using an in vitro method

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