“…In this matter, the wide-bore column with a thick stationary phase film is the best compromise between the amount injected and the separation capability. MD chromatography, especially in heart-cut mode, coupling two or more columns equipped with different stationary phases, has also demonstrated its suitability for enhancing the peak capacity in prep-GC applications [18]. In recent years, authors exploited different columns for coupling {e.g., apolar-polyethylene glycol [26], apolar-50% diphenyl [29], and apolar-polyethylene glycol-medium polarity IL [31,35,36]}.…”