Preparative Chromatography Techniques

Hostettmann, Kurt; Marston, Andrew; Hostettmann, M.

doi:10.1007/978-3-662-02492-8

Cited by 71 publications

(34 citation statements)

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“…The analysis of natural compounds presents major challenges due to the great variety and reactivity of these compounds [25,26]. The analysis is facilitated by using modern separation and detection methods, such as hyphenated techniques of HPLC with MS, UV/Vis, or NMR spectroscopy.…”

Section: Analytical Methods In Research Of Natural Compoundsmentioning

confidence: 99%

Natural Products in the Process of Finding New Drug Candidates

Vuorela¹,

Leinonen²,

Saikku³

et al. 2004

CMC

279

187

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Natural derived or originated compounds still play a major role as drugs, and as lead structures for the development of synthetic molecules. About 50% of the drugs introduced to the market during the last 20 years are derived directly or indirectly from small biogenic molecules. In the future, natural products will continue to play a major role as active substances, model molecules for the discovery and validation of drug targets. A multidisciplinary approach to drug discovery involving the generation of truly novel molecular diversity from natural product sources, combined with total and combinatorial synthetic methodologies provides the best solution to increase the productivity in drug discovery and development. Screening for new drugs in plants implies the screening of extracts for the presence of novel compounds and an investigation of their biological activities. It is currently estimated that approximately 420,000 plant species exist in nature. For the purpose of lead discovery, or for the scientific validation of a traditional medicinal plant or a phytopharmaceutical, active principals in complex matrices need to be identified. Therefore, the interfacing of biological and chemical assessment becomes the critical issue. Drug discovery from plants can be guided by epidemiologic studies facilitated with computer assisted HPLC microfractionation and microplate technology. Epidemiologic studies have shown that high dietary flavonoid intake may be associated with decreased risk for cardiovascular disease. Chlamydia pneumoniae is a common human pathogen and epidemiological and clinical studies have shown a connection between chronic C. pneumoniae infection, atherosclerosis and the risk of myocardial infarction. We will present here the detection of natural compounds active against C. pneumoniae as an example.

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Section: Analytical Methods In Research Of Natural Compoundsmentioning

confidence: 99%

Natural Products in the Process of Finding New Drug Candidates

Vuorela¹,

Leinonen²,

Saikku³

et al. 2004

CMC

279

187

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“…Combination of PLC and various methods of preparative CLC (column liquid chromatography) The introduction of precoated preparative plates with smaller particle size distributions and various types of stationary phases (e.g., RP-18, Sephadex) is essential. Hostettmann et al (83) tried to prepare preparative octadecyl layers, but the technique still has to be perfected. A real novelty to appear in recent years has been the taper plate; this dramatically increases resolution, especially in the lower R f range.…”

Section: Trends In Preparative Layer Chromatographymentioning

confidence: 99%

“…The normal chamber was used in more than 95% of the separations, although efforts are being made to introduce separations by the CLC-5 technique (71). Although the new types of chambers (micro, ultramicro, and planar column) were introduced relatively recently, their efficiency has been demonstrated for various classes of substances (9,(82)(83)(84).…”

Section: Nyiredymentioning

confidence: 99%

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Preparative Layer Chromatography

Nyiredy¹

2003

Handbook of Thin-Layer Chromatography

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“…Resolution is also impaired if the load is too high, column overloading arising from either an excessive sample volume or excessive sample mass. The loading capacity depends in turn on variables such as column radius, column length, particle diameter, and packing density of the support (9). In this work, in order to separate preparatively pure EGCG, we considered the maximum allowable load with higher purity of EGCG by changing the compositions of mobile phase at a constant injection volume.…”

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confidence: 99%

Preparative separation of EGCG from Korean green tea by high performance liquid chromatography

Hong

Kim

Row

2002

Separation Science and Technology

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Among catechin compounds abundant mainly in green tea, (2)epigallocatechin gallate (EGCG) has antioxidative and anticarcinogenic properties. Korean green tea (Bosung, Chonnam) was used as a feed sample. The separation was done by a C 18 reversedphase preparative column (22 £ 250 mm) with mobile phases of 0.1% acetic acid in water, acetonitrile, methanol, and ethyl acetate considered. The injection volume and the flow rate of mobile phase were fixed at 400 m L and 16 mL/min, respectively. To purify EGCG preparatively, the effluent was collected from the column outlet and concentrated, and analyzed by analytical column (3.9 £ 300 mm). From the experimental results, the optimum mobile phase condition for separating EGCG from Korean green tea was 0.1% acetic acid in water/acetonitrile/methanol/ethyl acetate (80/16/1/3 vol.%). In the experimental condition, 0.6 mg of EGCG was obtained purely. 1631

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Preparative Chromatography Techniques

Cited by 71 publications

References 0 publications

Natural Products in the Process of Finding New Drug Candidates

Natural Products in the Process of Finding New Drug Candidates

Preparative Layer Chromatography

Preparative separation of EGCG from Korean green tea by high performance liquid chromatography

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