Preparative Cell Electrophoresis

Streaming Potential Measurements of Biosurfaces

“…The decrease in f-potential may have been due to the effect of Giemsa stain on the cell periphery or the altered morphology of the cells. Giemsa is composed of Azure II, Eosin, glycerin, and methanol and any of these may have been the cause of the decrease in L Recently, a technique of evaluating cells cultured in capillary tubes utilizing electroosmosis has been reported by Fike and Van Oss (31). The advantage of this approach is that the cells may be evaluated for long times in the living state; however, the disadvantage is that the ionic strength must be an order of magnitude lower than physiological to obtain accurate measurements.…”

A new density gradient system for the separation of human red blood cells

“…A 40-fold enrichment in reticulocytes can be obtained in the top layers, with a great improvement of specific activity in labelling procedures of newly synthesized globin chains.The method is simple, rapid, inexpensive, reliable, nontoxic for erythrocytes, and is suitable for globin synthesis and other studies of erythrocyte metabolism.The separation of erythrocytes into age-dependent fractions, and the preparation of blood samples highly enriched in reticulocytes are problems of great interest in hematology. So far they have been only partially solved by a number of different approaches: serial osmotic hemolysis [ l ] , phase partitioning [2], electrophoresis [3,4], and buoyant density distribution [5-91 . has been performed utilizing many different substances, such as bovine serum albumin [6,9] , gum acacia [lo], phthalate esters [ l l ] , Ficoll [12], Ludox with polyvinylpyrrolidone [ 131 , dextran [ 141 , arabino-galactan [ 151 , Renografin with Ficoll [ 161 , and Renografin with arabino-galactan [17] .Density-gradient centrifugation has been the most commonly used technique, and…”