Preparation, structure, Cytotoxicity and Mechanism of Action of ferritin-Pt(II) Terpyridine Compound Nanocomposites

Abstract

Aim: A Pt(II)-terpyridine compound, bearing two piperidine substituents at positions 2 and 2' of the terpyridine ligand (1), is highly cytotoxic and shows a mechanism of action distinct from cisplatin. 1 has been incorporated within the ferritin nanocage (AFt). Materials & methods: Spectroscopic and crystallographic data of the Pt(II)-AFt nanocomposite have been collected and in vitro anticancer activity has been explored using cancer cells. Results: Pt(II)-containing fragments bind His49, His114 and His132. Pt(II)-AFt nanocomposite is less cytotoxic than 1, but it is more toxic than cisplatin at high concentrations. The Pt(II)-AFt nanocomposite triggers necrosis in cancer cells, as free 1 does. Conclusions: Pt(II)-AFt nanocomposites are promising vehicles to deliver Pt-based drugs to cancer cells.

Arsenoplatin-Ferritin Nanocage: Structure and Cytotoxicity

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Encapsulation of the Dinuclear Trithiolato‐Bridged Arene Ruthenium Complex Diruthenium‐1 in an Apoferritin Nanocage: Structure and Cytotoxicity

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Proanthocyanidin Encapsulated in Ferritin Enhances Its Cellular Absorption and Antioxidant Activity

“…The reversible process has been widely employed to encapsulate small molecules within its interior . Furthermore, apoferritin has been employed widely to encapsulate small molecules into internal cavity based on the pH-dependent disassembly and reassembly properties. − …”