Preparation, structural and spectroscopic characterization of chitosan membranes containing allantoin

Menezes, Jane Eire Silva Alencar de; Santos, Hélcio Silva dos; Ferreira, Maria Kueirislene Amâncio; Magalhães, Francisco Ernani Alves; Silva, D.S. da; Bandeira, Paulo Nogueira; Saraiva, G.D.; Pessoa, Otília Deusdênia Loiola; Ricardo, Nágila Maria Pontes Silva; Cruz, Beatriz Gonçalves; Teixeira, Alexandre Magno Rodrigues

doi:10.1016/j.molstruc.2019.126968

Cited by 26 publications

(18 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another study was implemented to investigate the performance of conventional as well as new argan oil enriched liposomes containing ALL as dermal drug delivery systems 15 . Recently, ALL was incorporated into CS membranes to evaluate its wound healing and tissue regeneration properties 16 . Consequently, different drug delivery systems (DDSs) are still required to be constructed and evaluated to potentiate and exploit the multilateral pharmacological bioactivities of ALL.…”

Section: Introductionmentioning

confidence: 99%

Formulation, optimization and characterization of allantoin-loaded chitosan nanoparticles to alleviate ethanol-induced gastric ulcer: in-vitro and in-vivo studies

Aman

Zaghloul

El-Dahhan

2021

Sci Rep

View full text Add to dashboard Cite

Allantoin (ALL) is a phytochemical possessing an impressive array of biological activities. Nonetheless, developing a nanostructured delivery system targeted to augment the gastric antiulcerogenic activity of ALL has not been so far investigated. Consequently, in this survey, ALL-loaded chitosan/sodium tripolyphosphate nanoparticles (ALL-loaded CS/STPP NPs) were prepared by ionotropic gelation technique and thoroughly characterized. A full 24 factorial design was adopted using four independently controlled parameters (ICPs). Comprehensive characterization, in vitro evaluations as well as antiulcerogenic activity study against ethanol-induced gastric ulcer in rats of the optimized NPs formula were conducted. The optimized NPs formula, (CS (1.5% w/v), STPP (0.3% w/v), CS:STPP volume ratio (5:1), ALL amount (13 mg)), was the most convenient one with drug content of 6.26 mg, drug entrapment efficiency % of 48.12%, particle size of 508.3 nm, polydispersity index 0.29 and ζ-potential of + 35.70 mV. It displayed a sustained in vitro release profile and mucoadhesive strength of 45.55%. ALL-loaded CS/STPP NPs (F-9) provoked remarkable antiulcerogenic activity against ethanol-induced gastric ulceration in rats, which was accentuated by histopathological, immunohistochemical (IHC) and biochemical studies. In conclusion, the prepared ALL-loaded CS/STPP NPs could be presented to the phytomedicine field as an auspicious oral delivery system for gastric ulceration management.

show abstract

Section: Introductionmentioning

confidence: 99%

Formulation, optimization and characterization of allantoin-loaded chitosan nanoparticles to alleviate ethanol-induced gastric ulcer: in-vitro and in-vivo studies

Aman

Zaghloul

El-Dahhan

2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Raman spectroscopy was performed to identify the structural characteristics of chitosan in CF and CP dressings compared to those of unmodified raw chitosan fiber. As shown in Figure 1, raw chitosan fiber generated vibrational lines at 1540 cm −1 , 1667 cm −1 , 1805 cm −1 , and 2434 cm −1 , which corresponded to C–N–H, C=O, and O–H carboxylic bending vibrations, respectively, and these were the main indications of the presence of chitosan [18,19,20]. Both CF and CP dressings display these vibrational characteristics but at lower intensity.…”

Section: Resultsmentioning

confidence: 99%

Biological Effects of Chitosan-Based Dressing on Hemostasis Mechanism

et al. 2019

View full text Add to dashboard Cite

There have been numerous recent advances in wound care management. Nevertheless, the assessment of hemostatic dressing is essential to enable surgeons and other physicians and healthcare professionals to make the correct decisions regarding the disposition of severe hemorrhage. Here, we investigated the relative efficacies of chitosan-based and conventional gauze dressings in a rat model of femoral artery hemorrhage and in patients with surgical wounds. Dressing effectiveness was evaluated based on hemostatic profiles, biocompatibility, antimicrobial activity, and blood factor responses in coagulation. Relative to standard gauze dressing, the chitosan fiber (CF) dressing treatment significantly shortened the time to hemostasis in injured rats. Moreover, the CF dressing significantly prolonged partial thromboplastin time, enhanced blood absorption, and reduced antithrombin production without altering the prothrombin ratio. Unlike regular gauze bandages, the CF dressing demonstrated remarkable antibacterial activity. The results of this study indicate the effectiveness of chitosan as a hemostatic dressing and elucidate its underlying mechanism. It is possible that chitosan surgical dressings could serve as first-line intervention in hospital emergency care for uncontrolled hemorrhage.

show abstract

“…FTIR Spectra Figure 1 showed the FTIR spectra of chitosan and chitosan derivatives. For chitosan, the characteristic peaks appeared at 3421 cm −1 (angular deformation of O-H and N-H), 2919 and 2881 cm −1 (-CH stretching), 1654 cm −1 (C=O stretching of amide band I), as well as 1596 cm −1 (amide band II) [29,30]. As for CTCS, the spectrum showed the introduction of the quaternary ammonium salt group and chloride acetyl group.…”

Section: Chemical Synthesis and Characterizationmentioning

confidence: 97%

Synthesis and Characterization of N,N,N-trimethyl-O-(ureidopyridinium)acetyl Chitosan Derivatives with Antioxidant and Antifungal Activities

Zhang

Tan

et al. 2020

Marine Drugs

View full text Add to dashboard Cite

Chitosan is an active biopolymer, and the combination of it with other active groups can be a valuable method to improve the potential application of the resultant derivatives in food, cosmetics, packaging materials, and other industries. In this paper, a series of N,N,N-trimethyl-O-(ureidopyridinium)acetyl chitosan derivatives were synthesized. The combination of chitosan with ureidopyridinium group and quaternary ammonium group made it achieve developed water solubility and biological properties. The structures of chitosan and chitosan derivatives were confirmed by FTIR, 1H NMR spectra, and elemental analysis. The prepared chitosan derivatives were evaluated for antioxidant property by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, hydroxyl radical scavenging ability, and superoxide radical scavenging ability. The results revealed that the synthesized chitosan derivatives exhibited improved antioxidant activity compared with chitosan. The chitosan derivatives were also investigated for antifungal activity against Phomopsis asparagus as well as Botrytis cinerea, and they showed a significant inhibitory effect on the selected phytopathogen. Meanwhile, CCK-8 assay was used to test the cytotoxicity of chitosan derivatives, and the results showed that most derivatives had low toxicity. These data suggested to develop analogs of chitosan derivatives containing ureidopyridinium group and quaternary ammonium group, which will provide a new kind of promising biomaterials having decreased cytotoxicity as well as excellent antioxidant and antimicrobial activity.

show abstract

Preparation, structural and spectroscopic characterization of chitosan membranes containing allantoin

Cited by 26 publications

References 29 publications

Formulation, optimization and characterization of allantoin-loaded chitosan nanoparticles to alleviate ethanol-induced gastric ulcer: in-vitro and in-vivo studies

Formulation, optimization and characterization of allantoin-loaded chitosan nanoparticles to alleviate ethanol-induced gastric ulcer: in-vitro and in-vivo studies

Biological Effects of Chitosan-Based Dressing on Hemostasis Mechanism

Synthesis and Characterization of N,N,N-trimethyl-O-(ureidopyridinium)acetyl Chitosan Derivatives with Antioxidant and Antifungal Activities

Contact Info

Product

Resources

About