Preparation, structural analysis and antioxidant activities of phosphorylated (1 → 3)-β-d-glucan

Mei, Xiaoguang; Tang, Qilin; Huang, Gangliang; Long, Rong; Huang, Hualiang

doi:10.1016/j.foodchem.2019.125791

Cited by 52 publications

(27 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DPPH assay is an electron transfer-based method which measures the capacity of an antioxidant (hydrogen donator) in the reduction of an oxidant. The DPPH method is frequently used as it allows for quick evaluation of antioxidant properties of samples [ 32 ]. The dark purple color of the solution changes to yellow when free radical scavengers exist.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing

Michalska‐Sionkowska

Warżyńska

Kaczmarek-Szczepańska

et al. 2021

Materials

View full text Add to dashboard Cite

Collagen possesses unique properties, e.g., biocompatibility, biodegradability, and non-toxicity. However, collagen material degrades too quickly and has low mechanical properties. One of the methods of polymers’ modification is mixing them to obtain blends. In this study, the influence of β-glucan for collagen material was analyzed. The interaction between the functional groups of the polymer was analyzed by ATR-FTIR (attenuated total reflection-fourier transform infrared) spectroscopy. The influence of β-glucan on mechanical properties was evaluated. The surface properties of materials were assessed using contact angle measurements and the topography of materials was evaluated by AFM (atomic force microscope). The structure of materials was analyzed according to SEM (scanning electron microscopy) pictures. Moreover, the DPPH-free radicals’ scavenging ability and biocompatibility against erythrocytes and HaCaT cells were evaluated. Collagen and β-glucan were bound together by a hydrogen bond. β-glucan addition increased the roughness of the surface of the film and resulted in a more rigid character of the materials. A small addition of β-glucan to collagen provided a more hydrophilic character. All the materials could swell in in vitro conditions and showed antioxidant activity. Materials do not cause erythrocyte hemolysis. Finely, our cytotoxicity studies indicated that β-glucan can be safely added at small (10% or less) quantity to collagen matrix, they sufficiently support cell growth, and the degradation products of such matrices may actually provide some beneficial effects to the surrounding cells/tissues.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Phosphorylated (1→3)-β-D-glucan could scavenge DPPH radicals in the dose–effect relationship [ 32 ]. Hussain et al noticed that the dose depended on an increase in DPPH scavenging for gamma-irradiated β-glucan [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing

Michalska‐Sionkowska

Warżyńska

Kaczmarek-Szczepańska

et al. 2021

Materials

View full text Add to dashboard Cite

show abstract

“…The DPPH free radical scavenging model is widely used for the quantitative determination of antioxidant capacities 37,38 . Figure 4(a) shows that trypsin extracted crude polysaccharides from the Lanzhou lily (the TRP treatment).…”

Section: Resultsmentioning

confidence: 99%

“…The DPPH free radical scavenging model is widely used for the quantitative determination of antioxidant capacities. 37,38 Figure 4(a) shows that trypsin extracted crude polysaccharides from the Lanzhou lily (the TRP treatment). The results also showed that Lanzhou lily polysaccharides exhibited obvious scavenging activity on DPPH radicals with respect to concentration dependency.…”

Section: Antioxidant Activity Dpph Radical Scavenging Activitymentioning

confidence: 99%

Structural characterization of Lanzhou lily (Lilium davidii var. unicolor) polysaccharides and determination of their associated antioxidant activity

Wang

Wei

et al. 2020

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUD: The Lanzhou lily (Lilium davidii var. unicolor) is the only Lilium species that is used for both culinary and medicinal purposes in China. Its bulbs contain various bioactive substances, such as polysaccharides, saponins and colchicine. Lanzhou lily polysaccharides are known to have anti-immunity, anti-tumor and anti-oxidation functions. RESULTS: The present study used a Box-Behnken design to optimize the ultrasound-assisted extraction of Lanzhou lily polysaccharides. Compared to other enzymes, trypsin significantly increased the polysaccharide yields, whereas the protein content of polysaccharides extracted with trypsin was the lowest. Monosaccharide mainly includes glucose (> 50%) and mannose (> 10%). 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity, chelating activity, total antioxidant capacity and hydroxyl radical scavenging activity of Lanzhou lily polysaccharides extracted with trypsin were stronger than those extracted without enzymes (control). Structural characteristics of Lanzhou lily polysaccharides extracted with trypsin and extracted without enzymes were characterized by scanning electron microscopy and nuclear magnetic resonance spectroscopy. When water extracted polysaccharide and trypsin extracted polysaccharide concentrations were 200 ∼g mL-1 , Raw264.7 proliferation rates were 101.69% and 159.41%, respectively. CONCLUSION: The Lanzhou lily polysaccharide was identified as ⊍-(1 → 6)-D-glucan. Consequently, the effects of both potential antioxidant and proliferative activity of trypsin are significant.

show abstract

“…The IR spectra of neat ⊍and ⊎-1,3-glucan are displayed at the top of Figs 7(a) and 7(b) respectively and they are consistent with previously reported spectra. 33,34 The peaks at 922, 1680 and 3020 cm −1 were assigned to ⊐(-C=CH-), ν(-C=C-) and ν(=CH), respectively, for both esters. Successful modification was confirmed by the disappearance or decrease of these vinyl peaks.…”

Section: Ftir Analyses Of the Modified Cast Filmsmentioning

confidence: 99%

Synthesis of α‐1,3‐ and β‐1,3‐glucan esters with carbon–carbon double bonds and their surface modification

Hori

Enomoto-Rogers

Kimura

et al. 2020

Polymer International

View full text Add to dashboard Cite

⊍-1,3-glucan and ⊎-1,3-glucan esters with carbon-carbon double bonds (C=C), namely, ⊍-1,3-glucan butenoate (⊍ 13 GB) and ⊎-1,3-glucan butenoate (⊎ 13 GB), were synthesized from 3-butenoic acid and trifluoroacetic anhydride. NMR analysis of the two esters revealed that the 3-butenoyl groups were partially transformed to 2-butenoyl groups. The total degree of substitution (DS total ) of the esters was calculated to be 3.0. According to gel permeation chromatography analysis, ⊍ 13 GB had a molecular weight (M w ) of 2.2 × 10 5 and ⊎ 13 GB had an M w of 11.0 × 10 5 , which was unexpectedly higher than that of the original glucan. This suggests that the ⊎ 13 GB chains were partially and intramolecularly crosslinked via the C=C bond. The ⊍ 13 GB and ⊎ 13 GB obtained had thermal degradation temperatures of 398 and 375°C, respectively, and glass transition temperatures of 117 and 119°C, respectively, which were higher than those of the corresponding saturated glucan butyrates. The surfaces of cast films of the esters were modified with 1H,1H,2H,2H-perfluorodecanethiol, n-dodecyl mercaptan or 3-mercapto-1,-2-propanediol via thiol-ene reactions. Attenuated total reflection Fourier transform infrared spectroscopy and scanning electron microscopy energy-dispersive X-ray spectroscopy analyses revealed that the surface of ⊍ 13 GB was more successfully modified with these thiol compounds than that of ⊎ 13 GB. The water contact angle of the surface of each cast film was measured to evaluate its hydrophobicity and hydrophilicity, and indicated the successful surface modification of the film by the thiol compounds

show abstract

Preparation, structural analysis and antioxidant activities of phosphorylated (1 → 3)-β-d-glucan

Cited by 52 publications

References 14 publications

Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing

Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing

Structural characterization of Lanzhou lily (Lilium davidii var. unicolor) polysaccharides and determination of their associated antioxidant activity

Synthesis of α‐1,3‐ and β‐1,3‐glucan esters with carbon–carbon double bonds and their surface modification

Contact Info

Product

Resources

About

Preparation, structural analysis and antioxidant activities of phosphorylated (1 → 3)-β-d-glucan

Cited by 52 publications

References 14 publications

Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing

Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing

Structural characterization of Lanzhou lily (Lilium davidii var. unicolor) polysaccharides and determination of their associated antioxidant activity

Synthesis of α‐1,3‐ and β‐1,3‐glucan esters with carbon–carbon double bonds and their surface modification

Contact Info

Product

Resources

About

Preparation, structural analysis and antioxidant activities of phosphorylated (1 → 3)-β-d-glucan