Preparation, physicochemical characterization and biological evaluation of cefodizime metal ion complexes

Auda, Sayed H.; Mrestani, Yahya; Nies, Dietrich H.; Groβe, Cornelia; Neubert, Reinhard H.H.

doi:10.1211/jpp.61.06.0007

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…The solubility of Ceftiofur hydrochloride changed drastically on its complexation with the metal ions under study which is in consonance with the results reported earlier for the complexes of other cephalosporins. 22 In conformity with the result of cefpodizime, 22 there was no significant change in the UV spectra of metal complexes from Ceftiofur hydrochloride. In case of IR spectrum it is found that compared to the characteristic band at 1764 cm -1 and 1606 cm -1 in case of Ceftiofur hydrochloride corresponding to the stretching frequency of the -lactam carbonyl (CO) group and the amidic carbonyl group, respectively, in metal complexes they appeared in the range of 1743-1777 cm -1 and 1635-1667 cm -1 ,respectively.…”

Section: Resultssupporting

confidence: 52%

“…9 The cephalosporin metal complexes have been analyzed by various spectral, thermal voltammetric and by HPLC methods. [10][11][12][13] A large number of reports on the effect of chelation on the biological activity of cephalosporin antibiotics [14][15][16][17][18][19][20][21][22] have appeared in the literature. In several cases, the results were found to be encouraging while in other cases, the activity was only comparable or somewhat lower.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and Antibacterial Activities of Metal Complexes of Ceftiofur

Tandon¹,

Tandon²,

Gupta³

2018

RJC

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The objective of the present study is to prepare a family of new monoanionic tridentate Ceftiofur metal complexes [M(Ceftiofur)Cl].xH2O, where M = Mn(II), Ca(II), Fe(III), Cu(II), Zn(II), Mg(II), Co(II) and Ni(II) and to characterize them by different physiochemical techniques. These Ceftiofur metal complexes are further screened for biological activities. Several physiochemical and spectroscopic methods like UV-Vis, 1 H-NMR, IR, elemental analysis, and Karl-Fischer titration were used to find out the actual stoichiometry and binding mode of these complexes. The complexes synthesized were subjected to anti-microbial activities using gram-positive as well as gram-negative strains. The chosen strains are Staphylococcus aureus (MTCC87), Bacillus subtilis (MTCC441), Escherichia coli (MTCC40), Proteus vulgaris (MTCC426), Pseudomonas aeuruginosa (MTCC2488), Bacillus cerus (MTCC430), Salmonella typhimurium (MTCC1253). The solubility of Ceftiofur hydrochloride was remarkably decreased in most of the commonly used solvents on its complexation with metal ions under study. The stoichiometric study of these synthesized complexes revealed 1:1 ratio of ligand to metal binding. Ceftiofur showed that on complexation the antibacterial study was affected. The Cu 2+ and Co 2+ complexes against Pseudomonas aeuruginosaand Co 2+, Mg 2+ and Ni 2+ complexes againstSalmonella typhimurium showed the highest antibacterial activity, whereas Ca 2+ complex showed the highest activity against Proteus vulgaris. Majority of the bacterial strains were found to be resistant to the complexes of Fe 3+ and Zn 2+ .

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Section: Resultssupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Synthesis and Antibacterial Activities of Metal Complexes of Ceftiofur

Tandon¹,

Tandon²,

Gupta³

2018

RJC

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“…Some cephalosporin antibiotics are known to form tight complexes with divalent metal ions, 27 including Zn 2+ , which suggested that the inhibition might result from an E-Zn 2+ –Ceph C enzyme-metal-bridged complex. However, further investigation showed that Zn 2+ alone (as the chloride salt) was also inhibitory (IC 50 Zn = 2.1 ± 0.2 μM, h = 1.2 ± 0.1; Fig.…”

Section: Resultsmentioning

confidence: 99%

A High-Throughput Enzyme-Coupled Assay for SAMHD1 dNTPase

Seamon

Stivers

2015

SLAS Discovery

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Sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) is a recently discovered enzyme that plays a central role in nucleotide metabolism and innate immunity. SAMHD1 has deoxyribonucleoside triphosphate (dNTP) triphosphohydrolase activity that depletes the dNTP substrates required for DNA synthesis in cells. The involvement of SAMHD1 in biological processes as varied as viral restriction, endogenous retroelement control, cancer, and modulation of anticancer/antiviral nucleoside drug efficacy makes it a valuable target for the development of small-molecule inhibitors. We report a high-throughput colorimetric assay for SAMHD1 dNTP hydrolase activity that takes advantage of Escherichia coli inorganic pyrophosphatase to convert PPPi to 3 Pi. The assay was validated by screening a library of 2653 clinically used compounds. Fifteen primary hits were obtained (0.57% hit rate); 80% of these were confirmed in a direct secondary assay for dNTP hydrolysis. The zinc salt of the antibiotic cephalosporin C was a potent inhibitor of SAMHD1 with an IC50 of 1.1 ± 0.1 μM, and this inhibition was largely attributable to the presence of zinc. The assay also screened a targeted library of nucleosides and their analogs, revealing that the antiviral drug acycloguanosine (acyclovir) is an inhibitor possessing excellent properties for future fragment-based drug development efforts.

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“…The metal ion contents were determined by complexmetric titration procedures and atomic absorption spectroscopy. The halogen content was determined by titration with standard Hg (NO 3 ) 2 solution using diphenylcarbazone indicator [24][25][26][27][28][29][30]. The analytical data and physical properties of the studied compounds are represented in Table 1 as previously reported [10][11].…”

Section: Synthesis Of Simple and Mixed Metallocephradines And Metallomentioning

confidence: 99%

In Vitro Biological Screening for Antimicrobial, DNA Cleavage Anti-Diabetic, Antioxidant, Anti-Inflammatory and Antihaemolytic of Some Metallocephalosporins

2017

International Journal of Research Studies in Biosciences

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Preparation, physicochemical characterization and biological evaluation of cefodizime metal ion complexes

Abstract: The antibacterial activity of cefodizime was not affected by complexation with Al3+ but it was reduced by complexation with the other tested metal ions against the bacteria under study.

Cited by 9 publications

References 24 publications

Synthesis and Antibacterial Activities of Metal Complexes of Ceftiofur

Synthesis and Antibacterial Activities of Metal Complexes of Ceftiofur

A High-Throughput Enzyme-Coupled Assay for SAMHD1 dNTPase

In Vitro Biological Screening for Antimicrobial, DNA Cleavage Anti-Diabetic, Antioxidant, Anti-Inflammatory and Antihaemolytic of Some Metallocephalosporins

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