Preparation, physical–chemical characterisation and cytocompatibility of calcium carbonate cements

Combes, Christèle; Miao, Baoji; Bareille, Reine; Rey, Christian

doi:10.1016/j.biomaterials.2005.09.026

Cited by 100 publications

(103 citation statements)

References 60 publications

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“…Mechanical, industrial, biological and clinical aspects are taken into consideration such as biocompatibility, bioactivity, recruiting capacity and induction of cellular differentiation. These materials cannot move on towards in vivo tests before a battery of in vitro tests [2][3][4] , among which immersion assays and tests with cellular cultures can be singled out. The creation of protocols of flexible and reliable tests permits reduction of costs and time in their application, assisting in the optimization of this crucial stage in the conversion of a prototype into a product.…”

Section: Introductionmentioning

confidence: 99%

Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Almeida¹,

Silva²,

Nakamura³

et al. 2015

Mat. Res.

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Aragonite is a metastable polymorph of calcium carbonate found in mollusk's shells, appearing in tiles and prismatic columns, cemented in a protein matrix -mainly proteins -that acts as a framework on which the aragonite is nucleated forming nacre, besides selecting the morphology of the nucleated cristaline phase. The presence of the mineralyzing organic matrix may affect osteoinductive properties of biogenic aragonite, hypothesis tested by combinated tests, comparing viability and bioactivity of biomineralizated aragonite and nacre. Bioactivity was observed by deposition of Ca-P (presumably calcium phosphate) on the surface of samples immersed in Simulated Body Fluid; biocompatibility was verified by adhesion with VERO cells; cytotoxicity and alkaline phosphatase activity assays were performed with human adipose stem cells (hASC). Samples were characterized by scanning electron microscopy and X-ray diffraction. Both materials showed similar behaviour on bioactivity assay; in contrast, exhibited different behaviours in the presence of hASC.

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Section: Introductionmentioning

confidence: 99%

Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Almeida¹,

Silva²,

Nakamura³

et al. 2015

Mat. Res.

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“…The TC-C and TC-MP biomaterials did not affect HPO 4 2-concentration in the media. The SH biomaterial caused a large increase in HPO 4 2-concentration in all media during the first two days of the experiment, and beginning from the 4 th day HPO 4 2-release was gradually inhibited.…”

Section: +mentioning

confidence: 82%

“…The SH biomaterial caused a large increase in HPO 4 2-concentration in all media during the first two days of the experiment, and beginning from the 4 th day HPO 4 2-release was gradually inhibited. The KMC-C biomaterial caused nearly complete sorption of HPO 4 2-ions from both types of media (Figure 4a and 4b). The HMPM biomaterial released extremely large amounts of HPO 4 2-ions in all media throughout the duration of the experiment.…”

Section: +mentioning

confidence: 97%

“…The KMC-C biomaterial caused nearly complete sorption of HPO 4 2-ions from both types of media (Figure 4a and 4b). The HMPM biomaterial released extremely large amounts of HPO 4 2-ions in all media throughout the duration of the experiment. It is worth noting that the highest ion concentration was detected on the second day of the measurements, and then desorption was gradually diminished with time.…”

Section: +mentioning

confidence: 98%

“…Calcium carbonate cements are also a subject of intensive studies [4]. Bioactive composite bone cements based on β-TCP-MPCM-C 3 S (tricalcium silicate) [5] or TTCP-DCPD-CSD (calcium sulfate dihydrate) [6] are reported to have significant clinical advantages.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

et al. 2013

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Calcium phosphate bioceramics have been studied as bone filler materials for years and have become a component of many commercial products. It is widely known that surface-reactive biomaterials may cause changes in the concentration of crucial ions in the surrounding environment, thereby affecting cell metabolism and viability. The aim of this study was to produce five cement-type biomaterials and characterize their phase composition using X-ray diffraction method, and porosity and pore size distribution using mercury intrusion porosimeter. We then evaluated ion interactions of the novel biomaterials with the surrounding environment (culture medium). A commercially available bone substitute, HydroSet™ (Stryker®), was used as a reference. MTT and NRU cytotoxicity tests were performed to assess the effect of changes in the concentration of crucial ions (calcium, magnesium, phosphate) on osteoblast metabolism and viability in vitro. Our study clearly indicated that various biomaterials demonstrated different ion reactivity and consequently may cause changes in ion concentration in the local environment. Critically low or high values of calcium, magnesium, and phosphate concentrations in the medium exerted cytotoxic effects on the cultured cells. Moreover, we discovered that the chemical composition of the culture medium had a substantial influence on ion interactions with biomaterials.

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Use of Vaterite and Calcite in Forming Calcium Phosphate Cement Scaffolds

Taş¹

Developments in Porous, Biological and Geopolymer Ceramics

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A series of novel orthopedic calcium phosphate (CaP+CaCO 3 ) cements have been developed. The common point in these cements was that they all utilized single-phase CaCO 3 (calcite or vaterite) in their powder components. The major phase in the end-product of these cements was carbonated, Ca-deficient, apatitic calcium phosphate, together with some varying amounts of unreacted CaCO 3 . Calcite powders used were needle-like or acicular in shape, whereas the vaterite powders were monodisperse, and spherical or ellipsoidal in shape. A new method for synthesizing spheroidal vaterite powders has also been developed. Setting solutions for calcite-based cement scaffolds were prepared by acid-base neutralization of concentrated H 3 PO 4 and NaOH. 0.5 M phosphate buffer solution was used to transform precipitated vaterite powders into CaP at 37º to 70ºC. Setting solutions possessed pH values ranging from 3 to 7.4 at room temperature. Resultant cements were micro-(5 to 50 micron pores) and macroporous (200 to 700 micron pores). In the macroporous cements, total porosity was variable from 20 to 45%. Setting times of those cements were adjustable over the range of 12 to 25 minutes. Compressive strengths of these cements varied from 2 to 3 MPa, depending on their porosity. CaP+CaCO 3 cements thus obtained had relatively high surface areas (30 to 85 m 2 /g) whose surfaces were covered with nanocrystallites similar in size to the nanoplatelets found in biological collagencalcium phosphate composites. Cement scaffolds were characterized by XRD, FTIR, SEM, ICP-AES, surface area and compressive strength measurements.

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Preparation, physical–chemical characterisation and cytocompatibility of calcium carbonate cements

Cited by 100 publications

References 60 publications

Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

Use of Vaterite and Calcite in Forming Calcium Phosphate Cement Scaffolds

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