Preparation, Optimization and Evaluation of Chitosan-Based Avanafil Nanocomplex Utilizing Antioxidants for Enhanced Neuroprotective Effect on PC12 Cells

Kurakula, Mallesh; N., Raghavendra Naveen; Patel, Bhaumik B.; Manne, Ravi; Patel, Dipal

doi:10.3390/gels7030096

Cited by 31 publications

(25 citation statements)

References 46 publications

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“…By using these concentrations, O-MNP was prepared to validate the experimental and also carry out the leftover evaluation tests. Relative error was noted to be less than 2%, confirming the accuracy of the design [52,53]. The reproducibility of the design was further supported by coefficients of variation values.…”

Section: Optimization Of Fe3o4@cs Mnpsupporting

confidence: 52%

“…High surface binding of gemcitabine (91.25%) was observed and this is a result of the use of CS. However, we are unable to extrapolate the same results with Fe3O4 owing to its diverse behavior at higher concentrations [53]. The surface morphology of the O-MNP formulation was found to be almost spherical and is in nanosize as per Figure 7.…”

Section: Surface Bindingand Semmentioning

confidence: 82%

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An Engineered Specificity of Anti-Neoplastic Agent Loaded Magnetic Nanoparticles for the Treatment of Breast Cancer

2021

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Nanoparticles have gained increased attention due to the prospection of drug delivery at target sites, thus limiting the systemic effects of the drugs. Their efficiency was further improved by adding special carriers such as magnetite (Fe3O4). It is one of the extensively used oxides of iron for both pharmaceutical and biomedical applications owing to its ease of preparation and biocompatibility. In this work, Gemcitabine magnetic nanoparticles were prepared using Fe3O4 and chitosan as the primary ingredients. Optimization was accomplished by Box–Behnken Design and factor interactions were evaluated. The desirability function approach was made to enhance the formulation concerning particle size, polydispersity index, and zeta potential. Based on this, optimized magnetic nanoparticles (O-MNP) were formulated with 300 mg of Fe3O4, 297.7 mg of chitosan, and a sonication time of 2.4 h, which can achieve the prerequisites of the target formulation. All other in vitro parameters were found to be following the requirement. In vitro cytotoxic studies for O-MNP were performed using cell cultures of breast cancer (MCF-7), leukemia (THP-1), prostate cancer (PC-3), and lung cancer (A549). O-MNP showed maximum inhibition growth with MCF-7 cell lines rather than other cell lines. The data observed here demonstrates the potential of magnetic nanoparticles of gemcitabine in treating breast cancers.

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Section: Optimization Of Fe3o4@cs Mnpsupporting

confidence: 52%

Section: Surface Bindingand Semmentioning

confidence: 82%

An Engineered Specificity of Anti-Neoplastic Agent Loaded Magnetic Nanoparticles for the Treatment of Breast Cancer

2021

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“…rhEGF-loaded NLC [ 18 , 20 , 25 , 26 , 27 ] and NLC alone (NLC-blank) were prepared with the hot melt homogenization technique according to a modified protocol described by Gainza and colleagues [ 15 ]. Briefly, an aqueous solution was prepared by adding 20 mg/mL rhEGF (Center for Genetic Engineering and Biotechnology, Havana, Cuba) to a warmed (50 °C) solution containing 0.67% ( w / v ) Poloxamer 188 (BASF, Ludwigshafen, Germany) and 1.33% ( w / v ) Tween 20 (Panreac Química, Barcelona, Spain).…”

Section: Methodsmentioning

confidence: 99%

Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers

et al. 2021

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Human skin keratinocyte primary cultures can be established from skin biopsies with culture media containing epithelial growth factor (EGF). Although current methods are efficient, optimization is required to accelerate the procedure and obtain these cultures in less time. In the present study, we evaluated the effect of novel formulations based on EGF-loaded nanostructured lipid carriers (NLC). First, biosafety of NLC containing recombinant human EGF (NLC-rhEGF) was verified in immortalized skin keratinocytes and cornea epithelial cells, and in two epithelial cancer cell lines, by quantifying free DNA released to the culture medium. Then we established primary cell cultures of human skin keratinocytes with basal culture media (BM) and BM supplemented with NLC-rhEGF, liquid EGF (L-rhEGF), or NLC alone (NLC-blank). The results showed that cells isolated by enzymatic digestion and cultured with or without a feeder layer had a similar growth rate regardless of the medium used. However, the explant technique showed higher efficiency when NLC-rhEGF culture medium was used, compared to BM, L-rhEGF, or NLC-blank. Gene expression analysis showed that NLC-rhEGF was able to increase EGFR gene expression, along with that of other genes related to cytokeratins, cell–cell junctions, and keratinocyte maturation and differentiation. In summary, these results support the use of NLC-rhEGF to improve the efficiency of explant-based methods in the efficient generation of human keratinocyte primary cell cultures for tissue engineering use.

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“…Inductively coupled plasma atomic emission spectroscopy (ICP-AES, PerkinElmer, Waltham, MA, USA) was used to obtain the soluble silica release profiles in PBS solution and high-performance liquid chromatography (HPLC, Shimadzu, Wolverton, UK) was used for the quantification of glycerol and glucose in degradation solutions. Experiments were performed in triplicates [102,103].…”

Section: In Vitro Degradation Of the Hydrogelmentioning

confidence: 99%

Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery

Jayash

Cooper

Shelton

et al. 2021

IJMS

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Hydrogels constructed from naturally derived polymers provide an aqueous environment that encourages cell growth, however, mechanical properties are poor and degradation can be difficult to predict. Whilst, synthetic hydrogels exhibit some improved mechanical properties, these materials lack biochemical cues for cells growing and have limited biodegradation. To produce hydrogels that support 3D cell cultures to form tissue mimics, materials must exhibit appropriate biological and mechanical properties. In this study, novel organic-inorganic hybrid hydrogels based on chitosan and silica were prepared using the sol-gel technique. The chemical, physical and biological properties of the hydrogels were assessed. Statistical analysis was performed using One-Way ANOVAs and independent-sample t-tests. Fourier transform infrared spectroscopy showed characteristic absorption bands including amide II, Si-O and Si-O-Si confirming formation of hybrid networks. Oscillatory rheometry was used to characterise the sol to gel transition and viscoelastic behaviour of hydrogels. Furthermore, in vitro degradation revealed both chitosan and silica were released over 21 days. The hydrogels exhibited high loading efficiency as total protein loading was released in a week. There were significant differences between TC2G and C2G at all-time points (p < 0.05). The viability of osteoblasts seeded on, and encapsulated within, the hydrogels was >70% over 168 h culture and antimicrobial activity was demonstrated against Pseudomonas aeruginosa and Enterococcus faecalis. The hydrogels developed here offer alternatives for biopolymer hydrogels for biomedical use, including for application in drug/cell delivery and for bone tissue engineering.

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Preparation, Optimization and Evaluation of Chitosan-Based Avanafil Nanocomplex Utilizing Antioxidants for Enhanced Neuroprotective Effect on PC12 Cells

Cited by 31 publications

References 46 publications

An Engineered Specificity of Anti-Neoplastic Agent Loaded Magnetic Nanoparticles for the Treatment of Breast Cancer

An Engineered Specificity of Anti-Neoplastic Agent Loaded Magnetic Nanoparticles for the Treatment of Breast Cancer

Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers

Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery

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