Preparation, Optimization, and Characterization of Topotecan Loaded PEGylated Liposomes Using Factorial Design

Vali, A.M.; Toliyat, Taiebeh; Shafaghi, Bijan; Dadashzadeh, Simin

doi:10.1080/03639040701385055

Cited by 29 publications

(18 citation statements)

References 32 publications

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“…The influence of the charged DSPG depended on the type of lipid that it was mixed with, improving DOPC's performance (sample 2; 78.4%) and diminishing DSPC's performance (sample 3; 56.5%). These results are in accordance with other published studies [47,52], and it was observed that encapsulation was affected by electrostatic interactions between the drug's peripheral surface and the polar head groups of phospholipids. In addition, there was a linear correlation between the lipid concentration and the encapsulation efficiency [53,54].…”

Section: Entrapment Efficiencysupporting

confidence: 83%

“…The same trends were obtained for the corresponding "empty" liposomes. Formulations with DSPE-PEG 2000 have less negative charge compared to liposomes without DSPE-PEG 2000 , a fact attributed to the "masking" of some of the anionic charges of DSPG by DSPE-PEG 2000 , which could explain the observed trend [40,47,50].…”

Section: ζ-Potentialmentioning

confidence: 80%

“…As indicated in Table 2 Liposome's ζ-potential values indirectly reflect the net charge of the vesicle surface. This is why the type of lipid used significantly influences the charge of the liposomal formulation [47]. Figure 2 depicts that the use of different type of lipids resulted in liposomal formulations with significant differences in ζ-potential values.…”

Section: ζ-Potentialmentioning

confidence: 99%

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Comparative Study of PEGylated and Conventional Liposomes as Carriers for Shikonin

Tsermentseli

Kontogiannopoulos

Papageorgiou

et al. 2018

Fluids

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Abstract:Liposomes are considered to be one of the most successful drug delivery systems.They apply nanotechnology to potentiate the therapeutic efficacy and reduce the toxicity of conventional medicines. Shikonin and alkannin, a pair of chiral natural naphthoquinone compounds, derived from Alkanna and Lithospermum species, are widely used due to their various pharmacological activities, mainly wound healing, antioxidant, anti-inflammatory and their recently established antitumor activity. The purpose of this study was to prepare conventional and PEGylated shikonin-loaded liposomal formulations and measure the effects of different lipids and polyethylene glycol (PEG) on parameters related to particle size distribution, the polydispersity index, the zeta potential, drug-loading efficiency and the stability of the prepared formulations. Three types of lipids were assessed (1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-distearoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DSPG)), separately and in mixtures, forming anionic liposomes with good physicochemical characteristics, high entrapment efficiencies (varying from 56.5 to 89.4%), satisfactory in vitro release profiles and good physical stability. The addition of the negatively charged DSPG lipids to DOPC, led to an increment in the drug's incorporation efficiency and reduced the particle size distribution. Furthermore, the shikonin-loaded PEGylated sample with DOPC/DSPG, demonstrated the most satisfactory characteristics. These findings are considered promising and could be used for further design and improvement of such formulations.

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Section: Entrapment Efficiencysupporting

confidence: 83%

Section: ζ-Potentialmentioning

confidence: 80%

Section: ζ-Potentialmentioning

confidence: 99%

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Comparative Study of PEGylated and Conventional Liposomes as Carriers for Shikonin

Tsermentseli

Kontogiannopoulos

Papageorgiou

et al. 2018

Fluids

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“…Liposomal formulations have been reported to stabilize Pac 5 and Top 6 . Hydrophilic stealthing shield of polyethylene glycol (PEG) renders long circulatory characteristics accounted to turning away RES (reticuloendothelial system) uptake [7][8][9] . In addition to enhanced blood circulation, warranted therapeutic efficacy can be accomplished in liposomes using surface-oriented targeting ligand like folate and imparting stimuli responsiveness viz.…”

Section: Introductionmentioning

confidence: 99%

Multipronged, strategic delivery of paclitaxel-topotecan using engineered liposomes to ovarian cancer

Jain

2015

Drug Development and Industrial Pharmacy

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“…From this investigation, the relatively high loading efficiency of ESB in the EPC liposomes and the release profile of the drug from these vesicles suggest that this lipid mixture would be a better choice for ESB entrapment. In contrast, higher encapsulation of hydrophilic compounds into the DPPC-and DSPC-liposomes compared to more fluid liposomes, which were shown by Vali et al (2008), might be explained by the higher rigidity of the former, which minimize the leakage of entrapped materials. In addition to the main lipid, 5 mol% PEG-DSPE and 10 mol% DOTAP were also added to all formulations in order to obtain steric-stabilized and positively charged liposomes.…”

mentioning

confidence: 56%

PEGylated estradiol benzoate liposomes as a potential local vascular delivery system for treatment of restenosis

Haeri

Sadeghian

Rabbani

et al. 2011

Journal of Microencapsulation

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This study was directed towards the preparation and optimization of PEGylated (PEG, poly(ethylene glycol)) estradiol benzoate (ESB)-loaded liposomes to be used for the treatment of restenosis by local vascular delivery. Various liposomal formulations were prepared by thin film hydration method followed by sonication. Response surface methodology was applied to study the influence of three different independent variables, on the response of entrapment efficiency (%EE). Liposomes were characterized in terms of size, zeta potential, %EE and release profile. Incorporation of ESB was higher in egg phosphatidylcholine (EPC) liposomes, whereas the drug was displaced from liposomes, as the cholesterol (Chol) content of liposome increased. The optimum formulation composed of EPC/dioleyloxy trimethylammonium propane/distearoylphosphatidylethanolamine-PEG2000 with a molar proportion of 8.5:1:0.5 had the highest EE. In vivo studies in the balloon-injured rat carotid arteries revealed the potential of ESB-loaded liposomes as efficient local and controlled drug delivery systems to reduce restenosis.

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Preparation, Optimization, and Characterization of Topotecan Loaded PEGylated Liposomes Using Factorial Design

Cited by 29 publications

References 32 publications

Comparative Study of PEGylated and Conventional Liposomes as Carriers for Shikonin

Comparative Study of PEGylated and Conventional Liposomes as Carriers for Shikonin

Multipronged, strategic delivery of paclitaxel-topotecan using engineered liposomes to ovarian cancer

PEGylated estradiol benzoate liposomes as a potential local vascular delivery system for treatment of restenosis

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