Preparation, optical characterization and stability of gold nanoparticles by facile methods

González, Verónica; Kharisov, Boris I.; Gómez, Idalia

doi:10.31349/revmexfis.65.690

Cited by 7 publications

(6 citation statements)

References 22 publications

(25 reference statements)

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“…Conversely, Mie and coworkers demonstrated that the oscillation modes depend on the particle size and as the size decreases, the maximum absorption also decreases, events that we observed when AuNPs were conjugated with BP [24,25].…”

Section: Discussionsupporting

confidence: 49%

“…In this study, we conjugated the AuNPs with the bioactive polyphenolic compounds (BP) of B. procumbens, which promote tissue repair and wound healing [8]. The characterization of AuNPs synthesis and AuNP-BP conjugation was performed using UV-visible spectrophotometry, FTIR spectra, DLS, TEM and electrophoretic light scattering to determine their optical properties, size, concentration, agglomeration state, hints on NP shape, surface composition, ligand binding, and hydrodynamic size [14,24]. The conjugation of BP to AuNPs shifted the nanoparticles' absorbance from 520 nm to 524 nm.…”

Section: Discussionmentioning

confidence: 99%

“…Higher concentrations of AuNP-BP displayed a peak at 668 nm, related to the chlorophyll α. Some reports have shown that higher concentrations of extracts could act as capping and stabilizing agents, such as phenolics, flavonoids, alkaloids, polysaccharides, saponins, tannins, and organic acids, leading to Au-hydro-complex formation rather than nanoparticles [24,25]. Thus, we chose a BP concentration of 1.6 mg/mL for optimal capping formation, while the concentration of nanoparticles, estimated by the Haiss method through the UV-vis spectrum, was 3.83 × 10 13 particles/mL.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Polyphenolic Compounds Nanostructurated with Gold Nanoparticles Enhance Wound Repair

Martínez-Cuazitl,

Gómez-García,

Pérez-Mora

et al. 2023

IJMS

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Gold nanoparticles (AuNPs) have been used in a wide range of applications, conferring to bio-molecules diverse properties such as delivery, stabilization, and reduction of the adverse effects of drugs or plant extracts. Polyphenolic compounds from Bacopa procumbens (B. procumbens) (BP) can modulate proliferation, adhesion, migration, and cell differentiation, reducing the artificial scratch area in fibroblast cultures and promoting wound healing in an in vivo model. Here, chemically synthesized AuNPs conjugated with BP (AuNP-BP) were characterized using UV-Vis, ATR-FTIR, DLS, zeta-potential, and TEM analysis. The results showed an overlap of the FTIR spectra of the polyphenolic compounds from B. procumbens adhered to the surface of the AuNPs. UV-vis analysis indicated that the average size of the AuNP-BP was 28 nm, while DLS analysis showed a size of 44.58 nm and, by TEM, a size of 16.5 nm with an icosahedral morphology was observed. These measurements suggest an increase in the size of the nanoparticles after conjugation with BP, compared to the sizes of 9 nm, 44.51 nm, and 14.17 nm for the unconjugated AuNPs, respectively. Furthermore, the zeta potential of the AuNPs, which was originally −36.3 ± 12.3 mV shifted to −18.2 ± 7.02 mV after conjugation with BP, indicating improved stability of the nanoparticles. Enhancement of the wound healing effect was evaluated by morphometric, histochemical, and FTIR changes in a rat wound excision model. Results showed that the nanoconjugation process reduced the BP concentrations by 100-fold to have the same wound healing effect as BP alone. Besides, histological and FTIR spectroscopy analyses demonstrated that AuNP-BP treatment exhibited better macroscopical performance, showing a reduction in inflammatory cells and an increased synthesis and improved organization of collagen fibers.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Polyphenolic Compounds Nanostructurated with Gold Nanoparticles Enhance Wound Repair

Martínez-Cuazitl,

Gómez-García,

Pérez-Mora

et al. 2023

IJMS

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“…This estimate was calculated using Equation (1) .

where M is 197 g/mol, which is the atomic mass of gold (Au), D is the average diameter of nanoparticles in nanometers, N A is Avogadro's number of element atoms (6.02214179 × 10 23 ), and ρ is the gold density (1.93 × 10 −20 g/nm 3 ) [ 68 ].…”

Section: Resultsmentioning

confidence: 99%

Electrochemical investigations and antimicrobial activity of Au nanoparticles photodeposited on titania nanoparticles

Al-Maydama,

Jamil,

Awad

et al. 2024

Heliyon

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“…However, stability is not only influenced by the selected surface chemistry, but also by different factors such as aggregation state, nanoparticle composition, shape and size, for which zeta potential, DLS and UV–visible techniques are crucial. For instance, a simple broad-spectrum technique used to determine nanoparticle stability over time consists of following the stability of the plasmon resonance signal using the UV–visible technique [ 69 ]. Due to all of these reasons, we employed DLS, zeta potential and UV–visible techniques to characterize the synthesized gold nanoparticles and nanocomplexes.…”

Section: Resultsmentioning

confidence: 99%

Gold Nanosystems Covered with Doxorubicin/DNA Complexes: A Therapeutic Target for Prostate and Liver Cancer

Giráldez-Pérez

Grueso

Montero-Hidalgo

et al. 2022

IJMS

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Different gold nanosystems covered with DNA and doxorubicin (Doxo) were designed and synthesized for cancer therapy, starting from Au@16-Ph-16 cationic nanoparticles and DNA–Doxo complexes prepared under saturation conditions. For the preparation of stable, biocompatible, and small-sized compacted Au@16-Ph-16/DNA–Doxo nanotransporters, the conditions for the DNA–Doxo compaction process induced by gold nanoparticles were first explored using fluorescence spectroscopy, circular dichroism and atomic force microscopy techniques. The reverse process, which is fundamental for Doxo liberation at the site of action, was found to occur at higher CAu@16-Ph-16 concentrations using these techniques. Zeta potential, dynamic light scattering and UV–visible spectroscopy reveal that the prepared compacted nanosystems are stable, highly charged and of adequate size for the effective delivery of Doxo to the cell. This fact is verified by in vitro biocompatibility and internalization studies using two prostate cancer-derived cell lines (LNCaP and DU145) and one hepatocellular carcinoma-derived cell line (SNU-387), as well as a non-tumor prostate (PNT2) cell line and a non-hepatocarcinoma hepatoblastoma cell line (Hep-G2) model used as a control in liver cells. However, the most outstanding results of this work are derived from the use of the CI+NI combined treatments which present strong action in cancer-derived cell lines, while a protective effect is observed in non-tumor cell lines. Hence, novel therapeutic targets based on gold nanoparticles denote high selectivity compared to conventional treatment based on free Doxo at the same concentration. The results obtained show the viability of both the proposed methodology for internalization of compacted nanocomplexes inside the cell and the effectiveness of the possible treatment and minimization of side effects in prostate and liver cancer.

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Preparation, optical characterization and stability of gold nanoparticles by facile methods

Cited by 7 publications

References 22 publications

Polyphenolic Compounds Nanostructurated with Gold Nanoparticles Enhance Wound Repair

Polyphenolic Compounds Nanostructurated with Gold Nanoparticles Enhance Wound Repair

Electrochemical investigations and antimicrobial activity of Au nanoparticles photodeposited on titania nanoparticles

Gold Nanosystems Covered with Doxorubicin/DNA Complexes: A Therapeutic Target for Prostate and Liver Cancer

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