Yttrium oxide is one of the rare earth metals. It has an elevated fluorescence emission reliability, which could have applications in biological imaging and photodynamic therapy, particularly in the biomedical field, where it is used as a versatile, multi-dimensional agent with antibacterial, and antioxidant activity. The goal of this research is to synthesize yttrium oxide nanoparticles (Y 2 O 3 nanoparticles) utilizing [Y(NO 3 ) 3 6H 2 O] as the precursor and the capping and reducing agent using Illicium verum (star anise) extract of flower and the investigation of biomedical applications which are the first report. Green synthesized nanoparticles were characterized with the assistance of a variety of techniques, such as dynamic light scattering, Fourier-transform infrared spectroscopy, X-ray diffraction, and field emission scanning electron microscopy. Y 2 O 3 nanoparticles exhibited strong antibacterial action against Staphylococcus aureus and Escherichia coli, with an inhibitory zone measuring 13 mm and 15 mm, respectively. Furthermore, these nanoparticles were able to significantly control bacterial growth in a concentration-and timedependent manner. In vitro antioxidant activity of green-synthesized Y 2 O 3 nanoparticles was determined using the DPPH technique, and a 50% scavenging efficiency was found at a concentration of 30 µg/mL.