Preparation of white cell‐depleted red cells for 42‐day storage using an integral in‐line filter

Abstract

A new blood pack system for the preparation of white cell-depleted red cells was studied. The system is a modified additive-solution quadruple-unit blood pack that incorporates a cellulose-acetate fiber depth filter in-line between the AS-3 additive bag and the CP2D collection bag. Mean +/- SD white cell removal from 156 units processed under standard production conditions was 97.7 +/- 2.7 percent; residual white cells were 1.1 +/- 1.0 x 10(8) per unit. Red cell loss was 10.0 +/- 1.0 percent (n = 43). Mean platelet removal was 80.9 percent from units from which platelet concentrates were not prepared (n = 47). Microaggregates did not form during storage, and hemolysis of filtered red cells was lower than that of unfiltered controls. Filtered AS-3 red cells stored for 42 days had a 51Cr survival of 80.1 +/- 5.7 percent (mean +/- SD) as compared with 78.9 +/- 6.2 percent for unfiltered controls (n = 17). This in-line filter system provides white cell-depleted, microaggregate-free red cells that can be stored for 42 days.

Evaluation of a New Prestorage Leukoreduction Filter for Red Blood Cell Units

“…Reduction in leukocyte content of the units was associ ated with reduced hemolysis and glucose consumption, as might be predicted from earlier work with prestorage leukoreduction [16,17]. While this trial of prestorage leukoreduction did not lead to a statistically significant improvement in red cell recovery, the observed recoveries were well above current requirements and suggest that extension of the red cell storage period (beyond 42 days) may be possible with prestorage leukoreduction [31], The LeukoNet…”

“…Furthermore, leukocytes may release enzymes and other constituents during storage that may be detrimental to red cell metabolism and viability. Small but statistically sig nificant decreases in hemolysis, increases in ATP levels and improvements in poststorage in vivo recovery have been re ported in association with prestorage leukoreduction [16,17]. In addition, conducting the filtration in a laboratory rather than at bedside permits quality control checks and tighter control over the performance of the technique that may lead to more uniform results [18,19].…”

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

“…Prevention of chill-and-fever reactions requires a level of fewer than 5 x 10x WBCs per unit red cell concentrate ( RCC ), whereas prevention of primary FILA al loimmunization is believed to require a reduction of WBCs to less than 5x 106 per unit [1], This can be achieved by fil tration of RCCs or platelet concentrates with currently available leukocyte depletion filters. Recently, in-line fil tration has been introduced, which allows early WBC de pletion of RCCs in closed systems before storage [3,4], However, filters and blood bags have to be centrifuged to-…”

Evaluation of a Red Cell Leukofilter Performance and Effect of Buffy Coat Removal on Filtration Efficiency and Post Filtration Storage

“…Most studies ascribe this improvement of RBC survival of filtered units to the lack of hydrolytic enzymes derived from WBCs dying in the storage container [26,27]. Small but statistically significant decreases in hemolysis, increases in ATP levels and improvements in poststorage in vivo recovery have been reported in association with prestorage leukofiltration [12,13].…”

“…Secondly, cytokines released from leukocytes during storage, may cause FNHTR in transfusion recipients [10,11]. Furthermore, release of enzymes by the leukocytes during storage may be detrimental to red cell metabolism and viability [12,13]. In addition, conducting the filtration in laboratory rather than at bedside, permits quality control checks and tighter control over the performance of the technique and leads to more uniform results [14].…”