Preparation of Uniform Nano Liposomes Using Focused Ultrasonic Technology

Abstract

Liposomes are microspheres produced by placing phospholipids in aqueous solutions. Liposomes have the advantage of being able to encapsulate both hydrophilic and hydrophobic functional substances and are thus important mediators used in cosmetics and pharmaceuticals. It is important for liposomes to have small sizes, uniform particle size distribution, and long-term stability. Previously, liposomes have been prepared using a homo mixer, microfluidizer, and horn and bath types of sonicators. However, it is difficult to produce liposomes with small sizes and uniform particle size distribution using these methods. Therefore, we have developed a focused ultrasound method to produce nano-sized liposomes with better size control. In this study, the liposome solutions were prepared using the focused ultrasound method and conventional methods. The liposome solutions were characterized for their size distribution, stability, and morphology. Results showed that the liposome solution prepared using focused ultrasonic equipment had a uniform particle size distribution with an average size of 113.6 nm and a polydispersity index value of 0.124. Furthermore, the solution showed good stability in dynamic light scattering measurements for 4 d and Turbiscan measurements for 1 week.

Development of Focused Ultrasound-Assisted Nanoplexes for RNA Delivery

“…This can give targetability problems if the primary function of the coating is to guide the particles to a particular place in the body. AFU can potentially overcome most of these problems because the sample does not come in contact with any extra materials like filter membranes, so the sample remains sterile, and the process is isothermal, so there is minimal fear of degradation of the molecules due to high temperatures [20,31]. This suggests the potential of the AFU-method to be optimized to make liposomal carrier particles that could be used in vivo.…”