Preparation of UDP- d -[U- 14 C]galacturonic acid and UDP- d -[6- 3 H]galactose with high specific activities

A simple and convenient method for the introduction of radiolabel onto C-6' of N-acetyllactosamine is described. 1-N-Benzyl-3-O-beta-D-galactopyranosyl-D-arabinosylamine (1) was synthesized from 3-O-beta-D-galactopyranosyl-D-arabinose as described by Lee and Lee. Compound 1 was oxidized with D-galactose oxidase, and the product reduced with KB3H4 to introduce the label at C-6'. After dilution with unlabeled material, the N-benzyl-3-O-beta-D-[6-3H]galactopyranosyl-D-arabinosylamine was converted into 2-(benzylamino)-2-deoxy-4-O-D-[6-3H]galactopyranosyl-D-glucononitrile , which was subjected to simultaneous hydrogenolysis of the benzylamino and nitrile groups. N-Acetylation of the amino group as described by Alais and Veyrières afforded the crystalline title compound in 63% yield.

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The effect of pH on enzyme-catalysed reactions: A computer program to determine pK values of enzyme ionisable groups from kinetic data

Gregory

Kinderlerer

1980

International Journal of Bio-Medical Computing

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Preparation of UDP- d -[U- 14 C]galacturonic acid and UDP- d -[6- 3 H]galactose with high specific activities

Cited by 8 publications

References 13 publications

A Facile Enzymatic Synthesis of Uridine Diphospho-[14C]galacturonic Acid

A Facile Enzymatic Synthesis of Uridine Diphospho-[14C]galacturonic Acid

Synthesis of N-acetyllactosamine containing a d-[6-3H]galactopyranosyl group

The effect of pH on enzyme-catalysed reactions: A computer program to determine pK values of enzyme ionisable groups from kinetic data

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