Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit Vaccine Delivery

Zhao, Lili; Bashiri, Sahra; Tóth, István; Skwarczynski, Mariusz

doi:10.1007/978-1-0716-1900-1_9

Cited by 2 publications

(3 citation statements)

References 23 publications

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“…As per the nanoparticulate support, LDHs are known as brucite-type anionic clays of positive structural charge, which is compensated with interlayer anions. , Beside the original lamellar structure, single-layer LDHs have attracted the attention of several researchers, since the anisotropy of such unilamellar nanosheets allows basic investigations and modeling of 2D quantum dots to be applied as building blocks in hybrid materials as well as for delivery of various biomolecules. − Besides, Alg and TMC were chosen to optimize charge balance and enhance enzyme adsorption during composite preparation, since these polyelectrolytes have been proven previously as suitable compounds in preparation of biocompatible nanohybrids. − The structural, functional, and colloidal stability of the system was fully characterized by scattering, microscopy, and spectroscopy techniques, while the antioxidant activity was assessed in biochemical tests and by advanced microscopy techniques in cellular experiments.…”

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confidence: 99%

“… 33 − 35 Besides, Alg and TMC were chosen to optimize charge balance and enhance enzyme adsorption during composite preparation, since these polyelectrolytes have been proven previously as suitable compounds in preparation of biocompatible nanohybrids. 36 − 38 The structural, functional, and colloidal stability of the system was fully characterized by scattering, microscopy, and spectroscopy techniques, while the antioxidant activity was assessed in biochemical tests and by advanced microscopy techniques in cellular experiments.…”

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confidence: 99%

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Confinement of Triple-Enzyme-Involved Antioxidant Cascade in Two-Dimensional Nanostructure

Szerlauth

Varga

Madácsy

et al. 2023

ACS Materials Lett.

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Application of antioxidant enzymes in medical or industrial processes is limited due to their high sensitivity to environmental conditions. Incorporation of such enzymes in nanostructures provides a promising route to obtain highly efficient and robust biocatalytic system to scavenge reactive oxygen species (ROS). Here, this question was addressed by confinement of superoxide dismutase (SOD), horseradish peroxidase (HRP), and catalase (CAT) enzymes into nanostructures containing polyelectrolyte building blocks (alginate (Alg) and trimethyl chitosan (TMC)) and delaminated layered double hydroxide (dLDH) nanoparticle support. The nanocomposite possessed excellent structural and colloidal stability, while antioxidant tests revealed that the enzymes remained active upon immobilization and the developed composite greatly reduced intracellular oxidative stress in two-dimensional cell cultures. Moreover, it effectively prevented hydrogen peroxide-induced double stranded DNA breaks, which is a common consequence of oxidative stress. The results provide important tools to design complex nanostructures with multienzymatic antioxidant activities for ROS scavenging.

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confidence: 99%

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confidence: 99%

Confinement of Triple-Enzyme-Involved Antioxidant Cascade in Two-Dimensional Nanostructure

Szerlauth

Varga

Madácsy

et al. 2023

ACS Materials Lett.

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“…Detection was conducted at 214 nm. Compounds were synthesized as previously reported: LCP−1 [ 26 ], lipoKALA [ 21 ], OTMC [ 23 ], lipoPEI [ 22 ], and TMC (degree of quaternization 65%) [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

The Development of Surface-Modified Liposomes as an Intranasal Delivery System for Group A Streptococcus Vaccines

et al. 2023

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Intranasal vaccine administration can overcome the disadvantages of injectable vaccines and present greater efficiency for mass immunization. However, the development of intranasal vaccines is challenged by poor mucosal immunogenicity of antigens and the limited availability of mucosal adjuvants. Here, we examined a number of self-adjuvanting liposomal systems for intranasal delivery of lipopeptide vaccine against group A Streptococcus (GAS). Among them, two liposome formulations bearing lipidated cell-penetrating peptide KALA and a new lipidated chitosan derivative (oleoyl-quaternized chitosan, OTMC) stimulated high systemic antibody titers in outbred mice. The antibodies were fully functional and were able to kill GAS bacteria. Importantly, OTMC was far more effective at stimulating antibody production than the classical immune-stimulating trimethyl chitosan formulation. In a simple physical mixture, OTMC also enhanced the immune responses of the tested vaccine, without the need for a liposome delivery system. The adjuvanting capacity of OTMC was further confirmed by its ability to stimulate cytokine production by dendritic cells. Thus, we discovered a new immune stimulant with promising properties for mucosal vaccine development.

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Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit Vaccine Delivery

Cited by 2 publications

References 23 publications

Confinement of Triple-Enzyme-Involved Antioxidant Cascade in Two-Dimensional Nanostructure

Confinement of Triple-Enzyme-Involved Antioxidant Cascade in Two-Dimensional Nanostructure

The Development of Surface-Modified Liposomes as an Intranasal Delivery System for Group A Streptococcus Vaccines

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