Preparation of Tc-99m-macroaggregated albumin from recombinant human albumin for lung perfusion imaging

Hunt, A. C.; Frier, M.; Johnson, RA; Berezenko, Stephen; Perkins, Alan C.

doi:10.1016/j.ejpb.2005.06.005

Cited by 25 publications

(13 citation statements)

References 6 publications

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“…The chemotherapeutic, anti-inflammatory, and hypoglycemic drugs bind to the intrinsic binding sites by non-covalent van der Waals forces electrostatic interactions or hydrophobic interactions 3, 6, 7. The classic examples of non-covalent interactions are radioactive 111 In- and 99m Tc-labeled macro-aggregated albumin ( 111 In-MAA and 99m Tc-MAA), which are widely used for gastrointestinal bleeding and lung perfusion by single photon emission computed tomography (SPECT) 8, 9. Albumin can also serve as a template or scaffold to enhance the distribution and pharmacokinetic profile of the drug to the tumor by the nanoparticles 10-12.…”

Section: Introductionmentioning

confidence: 99%

Versatile and Finely Tuned Albumin Nanoplatform based on Click Chemistry

Park¹,

Song²,

Kim³

et al. 2019

Theranostics

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Albumin is one of the most attractive nanoplatforms for targeted imaging and drug delivery due to its biocompatibility and long circulation half-life. However, previously reported albumin-based nanoplatforms have shown inconsistent blood circulation half-life according to the modified methods, and the affecting factors were not well evaluated, which could hamper the clinical translation of albumin-based nanoplatforms. Herein, we developed a finely tuned click-chemistry based albumin nanoplatform (CAN) with a longer circulation half-life and an efficient tumor targeting ability. Methods: CAN was synthesized in two steps. First, albumin was conjugated with ADIBO-NHS (albumin-ADIBO) by reacting albumin with various molar ratios of ADIBO. The number of attached ADIBO moieties was determined using matrix-assisted laser desorption ionization time of flight (MALDI-TOF). Second, the desired modalities including azide-functionalized chelator, a fluorescence dye, and folate were incorporated into albumin-ADIBO using strain-promoted alkyne-azide cycloaddition reaction (SPAAC reaction). The biodistribution and targeting efficiency of functionalized CANs were demonstrated in mice. Results: The degree of functionalization (DOF) and resulting in vivo biodistribution was controlled precisely using the click chemistry approach. Specifically, the numbers of attached azadibenzocyclooctyne (ADIBO) moieties on albumin, the DOF, were optimized by reacting albumin with varying molar ratios of ADIBO with a high reproducibility. Furthermore, we developed a simple and efficient method to estimate the DOF using UV - visible spectrophotometry (UV-vis), which was further validated by matrix-assisted laser desorption ionization time of flight (MALDI-TOF). The biodistribution of CAN could be controlled by DOF, and CAN with an optimized DOF showed a long circulation half-life (> 18 h). CAN was further functionalized using a simple click chemistry reaction with an azide functionalized chelator, a fluorescence dye, and folate. 64 Cu- and folate-labeled CAN ( 64 Cu-CAN-FA) showed effective and specific folate receptor targeting in vivo , with an over two-fold higher uptake than the liver at 24 h post-injection. Conclusions: Our development from the precisely controlled DOF demonstrates that an optimized CAN can be used as a multifunctional nanoplatform to obtain a longer half-life with radioisotopes and ligands, and provides an effective method for the development of albumin-based tumor theranostic agents.

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Section: Introductionmentioning

confidence: 99%

Versatile and Finely Tuned Albumin Nanoplatform based on Click Chemistry

Park¹,

Song²,

Kim³

et al. 2019

Theranostics

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“…99m Tc-MAA particles have diameter of about 10-50 μm while, pre-capillaries and capillaries have a mean diameter of 20-25 μm and 8 μm, respectively. Therefore, intravenous injection of 99m Tc-MAA particles block the blood flow to the distal region of lung by physically trapped in arteriocapillary beds as shown in Figure 4 [19,25].…”

Section: Capillary Blockadementioning

confidence: 99%

Localization Mechanisms of Radiopharmaceuticals

Komal¹,

Nadeem²,

Faheem³

et al. 2021

Medical Isotopes

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Scintigraphic techniques have opened a new era of developments in the localization of infectious and cancerous foci. Diseases area targeting mechanisms of radiopharmaceuticals encompasses visualization, characterization, and measurement of physiological and biological functioning at targeted sites in addition to measure the area and density of the disease. The accumulation of a radiopharmaceutical at specific organ is based upon numerous processes such as enzymatic interactions, receptor binding site, transport of chemical species and elimination of damaged cells from circulation by a normal metabolic process. PET and SPECT are developing scanning techniques that provides effective diagnostic tool to identify pathophysiology of diseased cells. In this chapter, we are exploring and explaining different mechanisms of radiopharmaceutical localization for imaging and therapeutic processes. The knowledge of these mechanisms will help to develop target based new radiopharmaceuticals using variety of medically used radioisotopes either for imaging or therapy of diseased cells.

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“…When the blood is labeled with 99m Tc, the radioactivity is mainly found on cellular compartment and, but it is also fixed on plasma proteins (Bernardo-Filho et al, 1990). Plasma proteins labeled with 99m Tc are used for evaluation of lung perfusion (Hunt et al, 2006), hepatic tumors and portal hemodynamic changes (Schneider et al, 2003). Authors have reported that extracts of some medicinal plants are capable to alter the labeling of blood constituents with 99m Tc (Oliveira et al, 2003;Abreu et al, 2006), while other extracts are not capable to interfere in this labeling (Fernandes et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells

Neves

Moreno

Rebello

et al. 2007

Braz. arch. biol. technol.

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Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock extract significantly (p<0.05) altered the %ATI on the blood compartments and the perimeter/area ratio of RBC, as well as, induced modifications on the shape of RBC. Alterations on membrane could justify the decrease of labeling of blood cells with 99mTc obtained in this study.
Arctium lappa (bardana) tem sido utilizada na medicina popular para o tratamento de processos inflamatórios. Constituintes sangüíneos marcados com tecnécio-99m (99mTc) são utilizados na medicina nuclear para obtenção de imagens. Neste trabalho foi avaliada a influência de um extrato de bardana na marcação de constituintes sangüíneos com 99mTc e na morfologia de hemácias. Amostras de sangue de ratos Wistar foram incubadas com extrato de bardana e o processo de radiomarcação de constituintes sangüíneos foi realizado. Plasma e células sangüíneas, frações solúvel e insolúvel do plasma e das células sangüíneas foram separadas, a radioatividade em cada fração foi contada e as porcentagens de radioatividade (%ATI) foram determinadas. A morfologia e a relação perímetro/área das hemácias foram avaliadas. A incubação de sangue com o extrato de bardana alterou significativamente (p<0.05) a %ATI a distribuição de radioatividade nos compartimentos plasmático e celular. A relação perímetro/área de hemácias, bem como a forma das hemácias também sofreram alterações Modificações na membrana poderiam justificar a diminuição da marcação das células sangüíneas com 99mTc obtidas neste estudo

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Preparation of Tc-99m-macroaggregated albumin from recombinant human albumin for lung perfusion imaging

Cited by 25 publications

References 6 publications

Versatile and Finely Tuned Albumin Nanoplatform based on Click Chemistry

Versatile and Finely Tuned Albumin Nanoplatform based on Click Chemistry

Localization Mechanisms of Radiopharmaceuticals

Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells

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