Preparation of surface molecularly imprinted polymeric microspheres and their recognition property for basic protein lysozyme

The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation

“…Over the past decades, molecularly imprinted polymer (MIP) has been successfully applied to a variety of small molecules [3,4]. Recent years, much attention has been paid to the imprinting of biomacromolecules and especially protein for its potential applications as biomaterials in separation, purification, biosensor, and mimicking enzyme and antibody [5][6][7][8][9][10][11]. However, the large molecular size, complexity, conformational flexibility, solubility, and numbers of functional groups of the protein make its imprinting face great challenges.…”

“…In order to achieve the effective imprinting, some methods have been developed, for example the surface imprinting [5,12,13] and epitope imprinting [14,15]. However, most of the current reports are concerned with small protein imprinting such as lysozyme (Lyz, MW 13.4 kDa) [5,[9][10][11]16,17], ribonuclease A (MW 13.7 kDa) [18] and cytochrome C (Cyt C, MW 12.3 kDa) [19,20]. Based on our previ-ous experiments in protein imprinting, large protein imprinting is more difficult because of its greater size and more complex structure.…”

Designing and preparation of cytisine alkaloid surface-imprinted material and its molecular recognition characteristics

“…A c c e p t e d M a n u s c r i p t 21 The adsorption experiments with column method were conducted using the NMIP-SASP/SiO 2 particles as non-imprinted material and MIP-SASP/SiO 2 particles as the imprinted material, respectively, and Fig. 10 and Fig.…”

Magnetic-graphene based molecularly imprinted polymer nanocomposite for the recognition of bovine hemoglobin

“…Some articles have reported molecular imprinting method at the surface of some support substrates, including silica beads, [19,20] chitosan, [21] membrane materials [22][23][24] and polymer particles [25] et al…”