Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury

Abstract

Traumatic muscle injury with massive loss of muscle volume requires intramuscular implantation of proper scaffolds for fast and successful recovery. Although many artificial scaffolds effectively accelerate formation and maturation of myotubes, limited studies are showing the therapeutic effect of artificial scaffolds in animal models with massive muscle injury. In this study, improved myotube differentiation is approved on stepwise stretched gelatin nanofibers and applied to damaged muscle recovery in an animal model. The gelatin nanofibers are fabricated by a two-step process composed of co-axial electrospinning of poly(ɛ-caprolactone) and gelatin and subsequent removal of the outer shells. When stepwise stretching is applied to the myoblasts on gelatin nanofibers for five days, enhanced myotube formation and polarized elongation are observed. Animal models with volumetric loss at quadriceps femoris muscles (>50%) are transplanted with the myotubes cultivated on thin and flexible gelatin nanofiber. Treated animals more efficiently recover exercising functions of the leg when myotubes and the gelatin nanofiber are co-implanted at the injury sites. This result suggests that mechanically stimulated myotubes on gelatin nanofiber is therapeutically feasible for the robust recovery of volumetric muscle loss. IntroductionVolumetric muscle loss (VML) is a form of severe skeletal muscle damage that can occur if there is more than 20% volume loss that

Construction of a myocardial patch with mesenchymal stem cells and poly(CL-co-TOSUO)/collagen scaffolds for myocardial infarction repair by coaxial electrospinning

“…26,27 Coaxial electrospinning techniques have been applied to develop composite core/shell nanofibers scaffolds, which could amplify the advantages of both synthetic and natural materials for tissue repair. 28–30 The present study used coaxial electrospinning technology to introduce natural collagen on the surface of the synthetic elastic material PCT (Fig. 3), where PCT was used as the core polymer to impart its elastic mechanical properties, and PCT/collagen (50 : 50) was used as the shell material to achieve favourable cell adhesion and proliferation for the stem cell cardiac patch.…”

Core–Shell Copper Azide-Based Nanofiber Films Prepared by Coaxial Electrospinning for MEMS Microinitiators

“…The CA films with high electrostatic safety suitable for MEMS microinitiators were then obtained via high-temperature carbonization and in situ azide processes. When compared to the traditional electrospinning technology, the coaxial electrospinning process can produce materials that cannot be electrospun individually into new nanofibers, as well as core–shell nanofibers. − This core–shell structure allows a carbon shell to be wrapped around the fibers’ surface, providing double protection for the CA and reducing its electrostatic sensitivity even further. Furthermore, the CA-based film can successfully detonate secondary explosives if the energy and sensitivity requirements are met, effectively alleviating the problem of powder samples being difficult to process and form.…”

A self-renewing biomimetic skeletal muscle construct engineered using induced myogenic progenitor cells

“…This technique facilitates orientation control of electrospun nano-scaled fibers on the substrate, guiding cellular growth akin to 3D bioprinting 18 . As a consequence of these attributes, electrospun substrates have been utilized to produce highly aligned myotubes using multiple materials including decellularized extracellular matrix (ECM) 22,23 , synthetic or natural polymers such as polyurethane 24 , poly(ε-caprolactone) (PCL) 25 , poly(lactic-co-glycolic acid) 26 , fibrin/alginate 27 and gelatin 11,28 . However, incorporation of self-renewing muscle stem cells into electrospun nano-scaled fibers that can regenerate muscle fibers has rarely been reported 10,11 .…”