Preparation of Sporoplasts for Studies of Pollen Physiology

Baldi, Bruce G.; Everard, John D.; Loewus, Frank A.

doi:10.1007/978-3-642-73271-3_16

Cited by 1 publication

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“…In addition to improving frequency of 2n pollen production, screening of 2n pollen is important to obtain polyploid plants, In case of sieving of pollens were effective for isolation of 2n pollen (Park et al 2013, Rathore et al 1991, Weisenseel and Jaffe 1976), but it is not the case in orchids due to the intrinsic nature of orchid sporogenesis, namely tetrad develops without isolation. Isolation by density gradient by Ficoll could be a solution (Baldi et al 1988, Midoro-Horiuti et al 1999.…”

Section: Discussionmentioning

confidence: 99%

Induction of 2n pollen with colchicine during microsporogenesis in <i>Phalaenopsis</i>

et al. 2022

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The induction of 2n pollen is an important technique for breeding polyploid plants. Here, we observed meiosis in the pollen mother cells (PMCs) of six Phalaenopsis cultivars and attempted to induce 2n pollen. The meiotic stage was related to flower bud length. During meiosis, Phalaenopsis cultivars with flower widths of approximately 20-40 mm and 50-60 mm had bud lengths of approximately 3-8 mm and 5-13 mm, respectively. The duration of meiosis ranged from 4.2 to 14 d. This was the first study to characterize meiosis of the PMCs of Phalaenopsis. The natural generation frequency of 2n pollen varied from 0.68% to 1.78%. Meiotic stage and colchicine concentration significantly affected the induction of 2n pollen. The most effective treatment for obtaining 2n pollen was 0.05% colchicine in the leptotene to zygotene stage for 3 d, which achieved a 2n pollen frequency of 10.04%.

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