Preparation of spirodiclofen monoclonal antibody and establishment of indirect competitive enzyme-linked immunosorbent assay

Jin, Yaqi; Jin, Zihui; Sun, Di; Peng, Yilin; Zhao, Qiyang; He, Yue; Li, Jing; Zhang, Yaohai; Cui, Yongliang

doi:10.1016/j.foodchem.2023.135936

Cited by 9 publications

(1 citation statement)

References 25 publications

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“…UV and SERS are usually utilized for rapid analysis of pollutants, but the sensitivity and selectivity of these methods need further improvement. In this aspect, an immunoassay, the enzyme-linked immunosorbent assay (ELISA), is the most commonly used method for detecting and quantifying residuals because of its simplicity, high-throughput detection capabilities, low cost, and high sensitivity and specificity [24][25][26][27]. Furthermore, icELISA is commonly employed for the screening of contaminants in the environment and food, playing a particularly significant role in grassroots food quality and safety monitoring.…”

Section: Introductionmentioning

confidence: 99%

An Enzyme Immunoassay Developed for the Determination of Methylene Blue in Aquatic Products Based on a Novel Hapten

Huang,

Yang,

Tang

et al. 2024

Chemosensors

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Methylene blue (MB) is a chemical dye which is used as an alternative drug for malachite green. In this study, based on computational chemistry, a novel hapten (TM) of MB was designed by comparing the conformational and electronic properties of MB. TM was synthesized and further conjugated with bovine serum albumin (BSA) and Ovalbumin (OVA). Then, the polyclonal antibodies were obtained by immunization with the immunogen of TM-BSA. Under optimal conditions, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed for the determination of MB, with an IC50 value of 41.5 μg L−1 and displaying a cross reaction of 78.2~88.9% with three different MB metabolins. The limit of detection (LOD) and the limit of quantitation (LOQ) for MB were validated to be 4.8 µg/kg and 6.0 µg/kg, respectively. Spiking experiments showed recoveries between 82.3% and 84.3%, with a relative standard deviation (RSD) of ≤6%. The results showed the generated polyclonal antibodies from the new hapten TM were reliable and could be used for detecting MB in fishery productions.

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Section: Introductionmentioning

confidence: 99%