Preparation of Spherical Cellulose Nanoparticles from Recycled Waste Cotton for Anticancer Drug Delivery

Abstract

Using natural waste resources as a starting material for the preparation of cellulose nanoparticles is a field of interest for novel pharmaceutical applications. This study aims to introduce cellulose nanoparticles for Doxorubicin (DOX) delivery to tumor cells. Cellulose nanocrystals were first produced from waste cotton by hydrochloric acid (1 M) hydrolysis, and then spherical cellulose nanoparticles were prepared by microemulsion method. The prepared particles were spherical with 300 nm in size, and the zeta potential was À 12.5 mV. The drug entrapment efficiency was ∼ 99 % (w/w). DOX loaded particles exhibited a biphasic release pattern at pH 7.4 phosphate buffer without initial burst effect. Release experiments demonstrated that DOX release was up to 34 days (97 %). In vitro cytotoxicity assay on human A549 (human lung adenocarcinoma) cell line was performed by MTT assay. The results show that prepared particles are effective on cancer cells. It is important in terms of economic and environmental problems that the material prepared for use in cancer drug release presented here is obtained from the waste cotton.

Effects of morphology and size of nanoscale drug carriers on cellular uptake and internalization process: a review

“…Spherical nanocarriers come in various shapes, from micellar to vesicular, which include internal structures with a hollow center and two continuous layers. 80 Barua et al reported higher non-specific cellular uptake of polystyrene (PS)-based nano-spheres by breast cancer cells (BT-474, SK-BR-3, and MDA-MB-231) compared with their nanorod and disc counterparts. 81 However, after covering them with the monoclonal antibody trastuzumab, it was shown that nanorods were more capable of being taken up by cells than spheres or discs.…”