Preparation of rabbit polyclonal antibodies against lupin storage proteins

Abstract

Doyle et al. (1992) noted that the N:S ratio for lupins is generally 20:1 whereas rumen bacteria require a ratio closer to

Emulsifying and Foaming Properties of Different Protein Fractions Obtained from a Novel Lupin Variety AluProt‐CGNA® (Lupinus luteus)

“…The molecular masses and isoelectric point (p I ) values of spots were manually determined by comparison with the mobilities of standard molecular markers (Mark 12 Unstained Standard; Life Technologies, Grand Island, N.Y., U.S.A.) and their distances from the acidic end of the IPG strip. Conglutin α, β, and γ were partially purified as described by Bush and Tai () with slight modifications, and used as standards in this study.…”

Functional and physicochemical properties of a protein isolate from Alu Prot -CGNA: A novel protein-rich lupin variety ( Lupinus luteus )

“…Protein bands on the gel were visualized by colloidal CBB staining, and the gel was digitalized using LAS 3000 (Fujifilm Co. Ltd., Tokyo, Japan). The standards used in this analysis, Osborn's albumin and α, β and γ conglutins, were partially purified following the method described by Bush and Tai (1994), with slight modifications.…”

Utilization of an Improved Methodology To Isolate Lupinus albus Conglutins in the Study of Their Sedimentation Coefficients

“…Several years later, Lupinus total globulins were fractionated by electrophoresis on cellulose acetate membranes into conglutins R, β, and γ (Blagrove and Gillespie, 1975). Subsequently, the conglutins were isolated by selective precipitation, by diethylaminoethyl-cellulose or FPLC/ Mono Q anion exchange chromatography, and by agarose or polyacrylamide gel electrophoresis (Duranti et al, 1981;Bush and Tai, 1994;Melo et al, 1994). However, as far as we are aware, no direct correlation has ever been made between the 11, 7, and 2 S protein components and the R-, β-, and γ-conglutins.…”