Preparation of porcine small intestinal submucosa sponge and their application as a wound dressing in full-thickness skin defect of rat

Kim, Moon Suk; Hong, Keum Duck; Shin, Hye Won; Kim, Seon Hwa; Kim, Soon Hee; Lee, Min Suk; Jang, Woo Young; Khang, Gilson; Lee, Hai Bang

doi:10.1016/j.ijbiomac.2005.03.013

Cited by 80 publications

(49 citation statements)

References 24 publications

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“…[45][46][47][48] However, there is a measurable loss of strength that occurs before transfer of mechanical function to the new host tissue. A common approach for increasing the strength of biologic scaffolds composed of ECM or components of ECM (e.g., purified type I collagen) has been the use of chemical crosslinking agents such as CDI, 24,[32][33][34] isocyanate, 49 or glutaraldehyde. [50][51][52] While chemical crosslinking does maintain the mechanical strength of the scaffold, it also necessarily alters the kinetics of degradation and has a deleterious effect on the biologic activity of the degradation products.…”

Section: Discussionmentioning

confidence: 99%

“…The SIS scaffolds designated as 14 C-X-SIS were prepared by immersing the multilaminate 14 C-SIS scaffolds in a solution of 10 mM CDI (Sigma, St. Louis, MO) in 90% acetone=10% deionized water (v=v) for 24 h. The chemical crosslinking agent used was CDI, which has been used widely used to crosslink commercially available ECM scaffolds. [32][33][34] To determine crosslinking efficacy, eight samples each of 14 C-SIS and 14 C-X-SIS were digested in triplicate in 27.3 U=mL bacterial collagenase type I (Sigma) for 72 h, and the amino acids released in the supernatant were quantified by the ninhydrin assay according to the manufacturer's protocol. The 14 C-X-SIS scaffolds were terminally sterilized by exposure to ethylene oxide at a dose of 500 mg=L=h for a 16 h cycle.…”

Section: Preparation Of CDI Crosslinkedmentioning

confidence: 99%

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Macrophage Participation in the Degradation and Remodeling of Extracellular Matrix Scaffolds

Valentin

Stewart‐Akers

Gilbert

et al. 2009

Tissue Engineering Part A

307

254

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Biologic scaffolds composed of extracellular matrix (ECM) are widely used to facilitate remodeling and reconstruction of a variety of tissues in both preclinical animal studies and human clinical applications. The mechanisms by which such scaffolds influence the host tissue response are only partially understood, but it is logical that the mononuclear macrophage cell population plays a central role. The present study evaluated the role of macrophages that derive from peripheral blood in the degradation of ECM scaffolds. An established rat body wall reconstruction model was used to evaluate the degradation of carbodiimide (CDI)-crosslinked scaffolds composed of porcine small intestinal submucosa (SIS), noncrosslinked SIS, and autologous body wall. To assess the role of circulating macrophages in the degradation process, the degradation of each scaffold was assessed with and without macrophage depletion caused by administration of clodronate-containing liposomes. Results showed that peripheral blood monocytes are required for the early and rapid degradation of both SIS scaffolds and autologous body wall, and that CDI crosslinked SIS is resistant to macrophage-mediated degradation.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of CDI Crosslinkedmentioning

confidence: 99%

Macrophage Participation in the Degradation and Remodeling of Extracellular Matrix Scaffolds

Valentin

Stewart‐Akers

Gilbert

et al. 2009

Tissue Engineering Part A

307

254

View full text Add to dashboard Cite

show abstract

“…[1][2][3][4][5][6][7][8][9][10][11][12] Such biological scaffolds have been surprisingly resistant to bacterial infection, [13][14][15][16] even in clinical applications that are at high risk for bacterial contamination. [17][18][19][20] Generally, naturally occurring biomaterials such as those composed of purified collagen or intact ECM are more resistant to bacterial infection than synthetic biomaterials. [13][14][15][16]21 Porcine-derived ECM composed of small intestinal sub-mucosa (SIS) has been successfully used as a resorbable biological scaffold for tissue-engineering applications in more than 500,000 human patients and has shown resistance to deliberate bacterial infection in preclinical studies.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Brennan

Reing²,

Chew³

et al. 2006

Tissue Engineering

209

133

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Biological scaffolds composed of extracellular matrix (ECM) have been shown to be resistant to deliberate bacterial contamination in preclinical in vivo studies. The present study evaluated the degradation products resulting from the acid digestion of ECM scaffolds for antibacterial effects against clinical strains of Staphylococcus aureus and Escherichia coli. The ECM scaffolds were derived from porcine urinary bladder (UBM-ECM) and liver (L-ECM). These biological scaffolds were digested with acid at high temperatures, fractionated using ammonium sulfate precipitation, and tested for antibacterial activity in a standardized in vitro assay. Degradation products from both UBM-ECM and L-ECM demonstrated antibacterial activity against both S. aureus and E. coli. Specific ammonium sulfate fractions that showed antimicrobial activity varied for the 2 different ECM scaffold types. The results of this study suggest that several different lowmolecular-weight peptides with antibacterial activity exist within ECM and that these peptides may help explain the resistance to bacterial infection provided by such biological scaffolds.

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“…Numerous studies have shown beneficial results in people in the treatment of wounds using PSIS [65][66][67] ; however, very few reports have thoroughly studied its application in clinical veterinary medicine. 20,29,68 Our purpose was to compare the effect of PSIS with a standard wound management protocol for large fullthickness wounds in dogs. We speculated that PSIS would provide a 3-dimensional matrix for cell migration and angiogenesis, thus promoting fibroplasia, epithelialization, and contraction.…”

mentioning

confidence: 99%

Effect of Porcine Small Intestinal Submucosa on Acute Full‐Thickness Wounds in Dogs

et al. 2008

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Objective-To evaluate the effects of porcine small intestinal submucosa (PSIS) on the healing of full-thickness wounds in dogs, specifically the appearance of granulation tissue, percent epithelialization and contraction, histologic variables of inflammation and repair, and aerobic culture results. Study Design-Prospective, controlled, experimental study. Animals-Purpose-bred, female dogs (n ¼ 10). Methods-Wounds were created bilaterally on the trunk; 1 side as a control and 1 treated with PSIS. First appearance of granulation tissue was recorded. Total wound area, open wound area, and epithelialized area were measured at 21 time points-wound contraction and percent epithelialization were calculated. Aerobic cultures were taken at 4 time points and wound biopsies at 8. Histologic features were graded into an Acute Inflammation Score and Repair Score. Results-There was no difference in first appearance of granulation tissue between PSIS-treated and control wounds. Wound contraction was significantly faster in control wounds as was percent epithelialization after day 21. Histologic Acute Inflammation Scores were significantly higher in PSIS-treated wounds compared with control wounds on days 2 and 6. There were no differences in Histologic Repair Scores between PSIS-treated and control wounds or in aerobic culture results. Conclusion-Wounds treated with PSIS contract more slowly, epithelialize less, and have more pronounced acute inflammation after implantation than control wounds. Clinical Relevance-Acute, full-thickness wounds in dogs do not benefit from treatment with PSIS. r

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Preparation of porcine small intestinal submucosa sponge and their application as a wound dressing in full-thickness skin defect of rat

Cited by 80 publications

References 24 publications

Macrophage Participation in the Degradation and Remodeling of Extracellular Matrix Scaffolds

Macrophage Participation in the Degradation and Remodeling of Extracellular Matrix Scaffolds

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Effect of Porcine Small Intestinal Submucosa on Acute Full‐Thickness Wounds in Dogs

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