Preparation of polyclonal antibody specific for NOR1 and detection of its expression pattern in human tissues and nasopharyngeal carcinoma

Xiang, Bo; Man, Yi; Wang, Li; Liu, Wei; Zhang, Wenling; Ouyang, Jue; Peng, Ya; Li, Wenjuan; Zhou, Ming; Liu, Huaying; Wu, Minghua; Wang, Rong; Li, Xiaoling; Li, Guiyuan

doi:10.1093/abbs/gmp064

Cited by 16 publications

(22 citation statements)

References 25 publications

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“…In the respiratory system, strong cytoplasmic immunostaining was observed in tracheal epithelium (Fig. 5C), which was similar to the reported NOR1 protein expression in human trachea (Xiang et al, 2009). However, mNOR1 protein was absent in the heart (Fig.…”

Section: Mouse Nor1 Protein Is Highly Expressed In Mouse Testis Epidsupporting

confidence: 88%

“…However, mNOR1 protein was absent in the heart (Fig. 5D) and lung (data not shown), which was also in accordance with hNOR1 expression level reported in these tissues (Xiang et al, 2009;Xiang et al, 2011).…”

Section: Mouse Nor1 Protein Is Highly Expressed In Mouse Testis Epidsupporting

confidence: 88%

“…Immunohistochemical staining of mNOR1 expression was performed as described previously (Xiang et al, 2009). Briefly, paraffinembedded tissue sections with 5 μm thickness were prepared from mouse tissues.…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Differential expression of oxidored nitro domain containing protein 1 (NOR1), in mouse tissues and in normal and cancerous human tissues

Xiang¹,

Wang²,

Li³

et al. 2012

Gene

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Section: Mouse Nor1 Protein Is Highly Expressed In Mouse Testis Epidsupporting

confidence: 88%

Section: Mouse Nor1 Protein Is Highly Expressed In Mouse Testis Epidsupporting

confidence: 88%

See 1 more Smart Citation

Differential expression of oxidored nitro domain containing protein 1 (NOR1), in mouse tissues and in normal and cancerous human tissues

Xiang¹,

Wang²,

Li³

et al. 2012

Gene

Self Cite

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“…Commonly used restriction enzymes, such as HindIII [16] and XhoI [17], form palindromes after digestion, allowing self-ligation to occur and reducing the ligation efficiency. Two different approaches were commonly used to prevent self-ligation.…”

Section: Discussionmentioning

confidence: 99%

A novel strategy for rapid construction of libraries of full-length antibodies highly expressed on mammalian cell surfaces

Zhou¹,

Chen²,

Li³

et al. 2010

ABBS

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Development of a versatile mammalian display system is essential for the selection of functional human antibodies with high affinities. Here we described a novel strategy for rapid construction of full-length antibody libraries that could be efficiently expressed on mammalian cell surfaces. The universal vector pDGB-HC-TM was constructed by inserting multiple cloning site unique sequences recognized by restriction endonucleases BsmBI, SfiI, and BstXI for the pop-in and pop-out of genes of interest. Cytomegalovirus promoter, a commonly used promoter for high expression of proteins in a variety of mammalian cells, was used to drive expression of the inserted antibody genes and a transmembrane domain from platelet-derived growth factor receptor was fused in frame to the C-terminus of heavy chain consistent region to anchor the antibody expressed on the mammalian cell surface. Using this strategy, we constructed a full-length human antibody display library. DNA sequence analysis and expression analysis indicated that the library constructed had a combinatory expressible, detectable diversity of 6.58 3 10 10 .

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“…NOR1 encodes a mitochondria-reactive tumor suppressor protein (MiTS-NOR1) [136] with an antioxidant function, which was able to inhibit the Warburg effect in NPC cells to inhibit tumor growth [137,138]. Intracellular reactive oxygen species can cause damage to cellular proteins, DNA, and organelles, leading to genomic instability and cancer.…”

Section: Important Genes In Npc Carcinogenesismentioning

confidence: 99%

Nasopharyngeal carcinoma: Advances in genomics and molecular genetics

Zeng

Huang

Zhang

et al. 2011

Sci. China Life Sci.

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Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma that arises in the epithelial lining of the nasopharynx [1]. This neoplasm has a notable ethnic and geographic distribution, being of high prevalence in southern China but rare in other parts of the world [2]. Familial clustering of NPC has been observed in diverse populations [3]. Elevated levels of circulating free Epstein-Barr virus (EBV) DNA and EBV-related antibodies in sera, as well as EBV DNA in tumor cells, have been consistently detected in individuals with NPC [4,5]. These studies have revealed that the risk factors of NPC are both environmental and genetic. How the risk factors interact, and the genes that are involved in the development of NPC, are not well understood [6].

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Preparation of polyclonal antibody specific for NOR1 and detection of its expression pattern in human tissues and nasopharyngeal carcinoma

Cited by 16 publications

References 25 publications

Differential expression of oxidored nitro domain containing protein 1 (NOR1), in mouse tissues and in normal and cancerous human tissues

Differential expression of oxidored nitro domain containing protein 1 (NOR1), in mouse tissues and in normal and cancerous human tissues

A novel strategy for rapid construction of libraries of full-length antibodies highly expressed on mammalian cell surfaces

Nasopharyngeal carcinoma: Advances in genomics and molecular genetics

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