Preparation of polyclonal antibodies against chemically synthesized ApxIA and ApxIVA toxins and their diagnostic efficacy in the experimentally injected mice

Pathogenesis and Prevention of Porcine Contagious Pleuropneumonia

“…Other researchers reported the establishment of the Apx-ELISA method, so that the Apx antibody cannot be detected in the APP carriers without infection symptoms (Rossic et al 2013). This is also in line with the study showing that no serum cross-reactive was present between antibodies to the recombinant Apx A protein and other Apx toxins, and no Apx antibody was detected from both the other standard positive serum and whole APP inactivated seedlings, but Apx antibody was detected from the serum of animals infected with live APP (Bode et al 2003;Li et al 2022). The ELISA method established with recombinant Apx protein in Previous reports have suggested that APP could detect antibodies to Apx in animals, but not in animals immunized with inactivated seedlings (Christensen 1982).…”

“…Apx I, expressed and secreted by serotypes 1,5,9,10 and 11, has strong hemolytic activity, and strong cytotoxic effects on alveolar macrophages and neutrophils, and is one of the potent protective antigens for APP (Meyns et al 2007). The production, activation, and secretion activity of ApxI is regulated by an operon that includes the above four complete CABD genes: ApxIC, ApxIA, ApxIB and ApxID, a typical operon of RTX toxin, and the product of ApxI is responsible for secretion to extracellular (Nkando et al 2012;Li et al 2022). Serotypes l, 5a, 5b, 9, 10 and 11 are clinically APP outbreak serotypes with severe lung injury and high mortality, and strong hemolytic and cytotoxic ApxI because they have intact ApxI, while serotypes 2,4,6,7,8 and 12, although not producing ApxI, have a truncated ApxI retaining the original promoter whose expressed products have secretory functional (Nielsen R et al 2000).…”