Preparation of platelet-rich plasma: National IADVL PRP taskforce recommendations

Abstract

The goal of these recommendations is to provide a framework to practitioners for implementing useful, evidence-based recommendations for the preparation of platelet-rich plasma (PRP) in various dermatological indications. The Indian Association of Dermatologists, Venereologists and Leprologists (IADVL) assigned the task of preparing these recommendations to its task force on PRP. A comprehensive literature search was done in the English language on the preparation of PRP across multiple databases. The grade of evidence and strength of recommendation was evaluated on the GRADE framework (Grading of Recommendation, Assessment, Development and Evaluation). A draft of clinical recommendations was developed on the best available evidence which was also scrutinized and critically evaluated by the IADVL Academy of Dermatology. Based on the inputs received, this final consensus statement was prepared. A total of 45 articles (meta-analyses, prospective and retrospective studies, reviews [including chapters in books], and case series) were critically evaluated and the evidence thus gathered was used in the preparation of these recommendations. This expert group recommends the use of double-spin manual method for the preparation of PRP. The recommended parameters for centrifuge are 100–300 g for 5–10 min for the first spin and 400–700 g for 10–17 min for the second spin. The recommended platelet concentration in PRP for the treatment of various dermatological conditions is 1–1.5 million platelets/μL. The activation of PRP is not required when it is injected into soft tissues.

Comparison of Single Centrifugation, Double Centrifugation and Turn down-Turn up Techniques for Platelet-Rich Plasma Quality

“…Compared to the SC technique, the DC is being the preferred technique for PRP preparation, as shown in a study for dermatologic use, due to its low cost, maximal platelet count, and adequate platelet volume. 20 The recovery efficiencies data measures the effect of the volume of plasma after the centrifugation process. 18 This measurement is part of the limitation of this study.…”

Platelet-rich plasma injection for symptomatic relief of disability associated with traumatic knee arthritis: a case report

“…7 The double-spin open method is the preferred method of preparation due to its lower cost and better platelet yield as compared to the single-spin method. 8 Nevertheless, the optimal centrifuge parameters have not been concluded yet. 8 A recent comprehensive consensus guideline on knee pain assigned a level 1 recommendation to safety and efficacy of intra-articular PRP for knee pain and improvement in function.…”

“…8 Nevertheless, the optimal centrifuge parameters have not been concluded yet. 8 A recent comprehensive consensus guideline on knee pain assigned a level 1 recommendation to safety and efficacy of intra-articular PRP for knee pain and improvement in function. 3 Studies have shown that improvement for pain and function decline starting at 6 to 9 months post-PRP injection; the optimal frequency of PRP injections remains unclear.…”

Plasma Cell Vulvitis Treated with Platelet-rich Plasma: A Case Report

“…3 Other treatment options with limited efficacy are imiquimod, oestrogen, misoprostol, interferon-alpha, cryotherapy, CO 2 laser, PRP, and surgical resection. 1,[3][4][5] Due to repeated treatment failures with topical steroids, immunomodulators, and cryotherapy, we opted for PRP to treat the patient. Platelets are a good source of growth factors like platelet-derived growth factor, insulin-like growth factor, vascular endothelial growth factor, and transforming growth factor-beta.6 These growth factors inhibit NF-kB and suppress proinflammatory cytokines like COX-2, and chemokine receptors like CXCR4, thus reducing inflammation.…”