Preparation of peppermint oil nanoemulsions: Investigation of stability, antibacterial mechanism and apoptosis effects

Liu, Qi; Gao, Yuan; Fu, Xuan; Chen, Wang; Yang, Jinghan; Chen, Ziyang; Wang, Zixuan; Zhuansun, Xiangxun; Feng, Jianguo; Chen, Yong

doi:10.1016/j.colsurfb.2021.111626

Cited by 52 publications

(33 citation statements)

References 44 publications

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“…In our research, the gene (TR16620_c0_g1) relative to peroxiredoxin activity was upregulated by 3.06-fold. The ROS-mediated response has been previously observed for nanoemulsion treatment (76,77). Similar results showed that genes (CAT1, SOD1, SOD4, SOD5, AOX2, and YHB1) showed upregulated tendency after peptide MAF-1A treatment, involved in oxidative stress response (78).…”

Section: Genes Related To Stress Responsesupporting

confidence: 81%

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

et al. 2021

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Penicillium digitatum is the most severe pathogen that infects citrus fruits during storage. It can cause fruit rot and bring significant economic losses. The continuous use of fungicides has resulted in the emergence of drug-resistant strains. Consequently, there is a need to develop naturally and efficiently antifungal fungicides. Natural antimicrobial agents such as clove oil, cinnamon oil, and thyme oil can be extracted from different plant parts. They exhibited broad-spectrum antimicrobial properties and have great potential in the food industry. Here, we exploit a novel cinnamaldehyde (CA), eugenol (EUG), or carvacrol (CAR) combination antifungal therapy and formulate it into nanoemulsion form to overcome lower solubility and instability of essential oil. In this study, the antifungal activity evaluation and transcriptional profile of Penicillium digitatum exposed to compound nanoemulsion were evaluated. Results showed that compound nanoemulsion had a striking inhibitory effect on P. digitatum in a dose-dependent manner. According to RNA-seq analysis, there were 2,169 differentially expressed genes (DEGs) between control and nanoemulsion-treated samples, including 1,028 downregulated and 1,141 upregulated genes. Gene Ontology (GO) analysis indicated that the DEGs were mainly involved in intracellular organelle parts of cell component: cellular respiration, proton transmembrane transport of biological process, and guanyl nucleotide-binding molecular function. KEGG analysis revealed that metabolic pathway, biosynthesis of secondary metabolites, and glyoxylate and dicarboxylate metabolism were the most highly enriched pathways for these DEGs. Taken together, we can conclude the promising antifungal activity of nanoemulsion with multiple action sites against P. digitatum. These outcomes would deepen our knowledge of the inhibitory mechanism from molecular aspects and exploit naturally, efficiently, and harmlessly antifungal agents in the citrus postharvest industry.

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Section: Genes Related To Stress Responsesupporting

confidence: 81%

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

et al. 2021

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“…High shear mixer (IKA, Germany) was used for the preparation of nanoemulsions as previously described. 30 First, coarse emulsion was prepared with mixing 5% (w/w) essential oil and surfactant by using a magnetic stirrer for 10 min at 1000 rpm at room temperature. Then, deionized water was added to the oil phase, the final volume was adjusted to 100% (w/w), and the mixture was treated by a high shear mixer at 12 000 rpm.…”

Section: Methodsmentioning

confidence: 99%

Preparation of eugenol nanoemulsions for antibacterial activities

Gao

Yan

et al. 2022

RSC Adv.

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“…The zeta potential of dispersed-phase droplets can affect the stability of the emulsion interface, especially in different application environments (whether or not effectively protect and enhance the absorption of substances in simulated gastrointestinal environments), thus indirectly affecting the load capacity and availability of delivery active ingredient. In particular, when the antibacterial emulsion loaded with antibacterial active substances plays a role, the zeta potential of dispersed phase droplets will directly affect the contact with Gram-positive bacteria and Gram-negative bacteria, thereby affecting the antibacterial performance of the loaded antibacterial substances [ 25 , 33 ].…”

Section: Applications On the Dispersed-phase Scalementioning

confidence: 99%

Progress in the Application of Food-Grade Emulsions

Jie

Chen

2022

Foods

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The detailed investigation of food-grade emulsions, which possess considerable structural and functional advantages, remains ongoing to enhance our understanding of these dispersion systems and to expand their application scope. This work reviews the applications of food-grade emulsions on the dispersed phase, interface structure, and macroscopic scales; further, it discusses the corresponding factors of influence, the selection and design of food dispersion systems, and the expansion of their application scope. Specifically, applications on the dispersed-phase scale mainly include delivery by soft matter carriers and auxiliary extraction/separation, while applications on the scale of the interface structure involve biphasic systems for enzymatic catalysis and systems that can influence substance digestion/absorption, washing, and disinfection. Future research on these scales should therefore focus on surface-active substances, real interface structure compositions, and the design of interface layers with antioxidant properties. By contrast, applications on the macroscopic scale mainly include the design of soft materials for structured food, in addition to various material applications and other emerging uses. In this case, future research should focus on the interactions between emulsion systems and food ingredients, the effects of food process engineering, safety, nutrition, and metabolism. Considering the ongoing research in this field, we believe that this review will be useful for researchers aiming to explore the applications of food-grade emulsions.

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Preparation of peppermint oil nanoemulsions: Investigation of stability, antibacterial mechanism and apoptosis effects

Cited by 52 publications

References 44 publications

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

Preparation of eugenol nanoemulsions for antibacterial activities

Progress in the Application of Food-Grade Emulsions

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