Preparation of Natural Hydroxyapatite from Bovine Femur Bones Using Calcination at Various Temperatures

Khoo, W. S.; Nor, Fethma M.; Ardhyananta, Hosta; Kurniawan, Denni

doi:10.1016/j.promfg.2015.07.034

Cited by 103 publications

(79 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2 demonstrates the XRD patterns of the raw bovine femur bone (B0) and calcined bovine femur bone at 900 °C (B900), 950 °C (B950), 1000 °C (B1000), 1050 °C (B1050) and 1100 °C(B1100). For the calcined samples, there were three main peaks, namely 211, 112 and 300 planes at 2θ near 31.8°, 32.2° and 32.9°, respectively [19,20]. It was evident that the intensity of diffraction peaks increased with the calcination temperature [21].…”

Section: X-ray Diffraction (Xrd)mentioning

confidence: 90%

See 1 more Smart Citation

Access to Optimal Calcination Temperature for Nanoparticle Synthesis from Hydroxyapatite Bovine Femur Bone Waste

Hammood¹,

Hassan²,

Alkhafagy³

2017

Nano BioMed ENG

View full text Add to dashboard Cite

Hydroxyapatite (HAp) is one of the vital and bioactive materials that are commonly used in biomedical field and concentrated in clinical area. It is a bio-ceramic powder synthesized by using different bio-waste materials such as bovine femur bone. In this study, the bovine femur bone powder was prepared to obtain nano powder. The purpose of this study was to reach the optimal temperature to obtain nanoparticles HAp. The resulted powder was calcinated in a furnace at different temperatures (900, 950, 1000, 1050 and 1100 °C) for 2 h at the heating rate of 10 °C/min and cooled slowly in a furnace. Results showed, the formation of pure HAp by the presence of peaks corresponding to (PO 4 ) 3-at 632/cm and OH -at 3,572/cm in fourier transformation infrared spectroscopy (FTIR). For the calcined samples, there were three main peaks of 211, 112 and 300 planes at 2θ near 31.8, 32.2 and 32.9 respectively. The amorphous raw bones were transformed into crystalline phase and the lattice parameters for HAp c and a were calculated in X-ray diffraction (XRD), Raman analysis showed that the calcination process removed the organic compound from the bovine femur bones matrix. Scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX) showed that the shape of the granules was irregular, containing quantities of oxygen, magnesium, sodium and carbon; the ratio of calcium to phosphate was calculated, Atomic force microscopy (AFM) showed that the particles sizes ranged from nanometers to microns. EDX result found that the calcium to phosphate ratio reached 1.7058 after calcination at 950 °C which was close to stoichiometric hydroxyapatite (1.67). This result implied the formation of pure HAp phase at 950 °C.

show abstract

Section: X-ray Diffraction (Xrd)mentioning

confidence: 90%

“…When the calcination temperature increased from 900 °C to 1100 °C, the grain size increased. This grain growth may be associated with the absorption of heat energy by the particles [19]. The image of SEM showed the formation of micro and sub-micro grains of HAp for calcined sample [23].…”

Section: X-ray Diffraction (Xrd)mentioning

confidence: 99%

Access to Optimal Calcination Temperature for Nanoparticle Synthesis from Hydroxyapatite Bovine Femur Bone Waste

Hammood¹,

Hassan²,

Alkhafagy³

2017

Nano BioMed ENG

View full text Add to dashboard Cite

show abstract

“…Sintering temperature has great influences on crystal structure. As sintering temperature increased, the crystallinity degree and crystallite size of hydroxyapatite also increase . However, decomposition of hydroxyapatite would occur when samples annealed at a high temperature .…”

Section: Resultsmentioning

confidence: 99%

The optimization of sintering treatment on bovine‐derived bone grafts for bone regeneration: in vitro and in vivo evaluation

Lin

et al. 2019

J Biomed Mater Res

View full text Add to dashboard Cite

Modifications of sintering temperature and treatment time of bovine‐derived bone grafts affect their physicochemical properties and further influence biological activity. Three different temperature sintered bovine‐derived bone grafts: group I (300 °C 3 h), group II (300 °C 3 h plus 530 °C 6 h), and group III (300 °C 3 h plus 1000 °C 2 h) and Bio‐Oss® were characterized and then compared in vitro for their effects on bone marrow stromal cells (BMSCs) migration, proliferation, and differentiation as estimated by cell migration assay, Alkaline phosphatase (ALP) activity assay, and Alizarin red staining. Further, the four bone grafts were implanted into the calvarial defects of rabbits to evaluate bone regeneration and graft degradation. The four deproteinized bovine‐derived bone grafts displayed different surface topography. Group II displayed the highest potential of attracting cells. Both groups I and II markedly promote BMSCs differentiation. After 6 and 12 weeks, defects grafted with groups I and II displayed a significant higher bone fraction than defects grafted with group III and Bio‐Oss®. Bone graft remnants remained in all four groups. Taken together, sintering at 300 °C for 3 h and sintering at 300 °C for 3 h with an addition of 530 °C for 6 h of bovine‐dervied bone grafts displayed potential use in bone regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:272–281, 2020.

show abstract

“…Sebagai contoh, komposisi dentin manusia terdiri daripada 45% HA, 25% air dan 30% matriks organik (Antonio 2003;Khoo et al 2015). HA bersifat bioserasi dan berkonduksian osteo (Mohd et al 2008).…”

Section: Pendahuluanunclassified

Sinteran Hidroksiapatit dalam Atmosfera Nitrogen untuk Peningkatan Sifat Mikrokekerasan

Leong

Muchtar²,

Tan

et al. 2017

JSM

View full text Add to dashboard Cite

Preparation of Natural Hydroxyapatite from Bovine Femur Bones Using Calcination at Various Temperatures

Cited by 103 publications

References 0 publications

Access to Optimal Calcination Temperature for Nanoparticle Synthesis from Hydroxyapatite Bovine Femur Bone Waste

Access to Optimal Calcination Temperature for Nanoparticle Synthesis from Hydroxyapatite Bovine Femur Bone Waste

The optimization of sintering treatment on bovine‐derived bone grafts for bone regeneration: in vitro and in vivo evaluation

Sinteran Hidroksiapatit dalam Atmosfera Nitrogen untuk Peningkatan Sifat Mikrokekerasan

Contact Info

Product

Resources

About