Preparation of monoclonal antibody to rabbit kidney gamma-glutamyl transpeptidase and its cross-reactivity analysis in immunohistochemistry.

Since Kohler and Milstein (9) developed a method to prepare the monoclonal antibody (McAb), the antibody has widely used in every field of biological science. As is well known, the McAb recognizes only one determinant of the antigen with high specificity and contains antibody class or subclass in the uniform pattern. Moreover, the hybridoma, once established, produces the homogeneous antibody molecule in the culture media continuously. On the other hand, McAb does not precipitate the antigen in the form of the immune complex of the large molecule, and shows cross-reactivity (11). In the application of McAb to immunohistochemical studies, it is necessary to

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Immunohistochemical study of .GAMMA.-glutamyl transpeptidase during the development and maturation of rat kidney.

Niu

Yamashita

Yasuda

1990

Acta Histochem. Cytochem

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Preparation of monoclonal antibody to rabbit kidney gamma-glutamyl transpeptidase and its cross-reactivity analysis in immunohistochemistry.

Cited by 3 publications

References 37 publications

Comparative biochemical and immunological studies on gamma-glutamyltransferases from human kidney and renal cell carcinoma applying monoclonal antibodies

Comparative biochemical and immunological studies on gamma-glutamyltransferases from human kidney and renal cell carcinoma applying monoclonal antibodies

Application of monoclonal antibodies to immunohistochemistry.

Immunohistochemical study of .GAMMA.-glutamyl transpeptidase during the development and maturation of rat kidney.

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