Preparation of Monoclonal Antibody for Melamine and Development of an Indirect Competitive ELISA for Melamine Detection in Raw Milk, Milk Powder, and Animal Feeds

Yin, Weiwei; Liu, Jinting; Zhang, Taichang; Li, Weihua; Liu, Wei; Meng, Meng; He, Fengtian; Yue, Wan; Chen, Feng; Wang, Shanliang; Xiao, Lu; Xi, Rimo

doi:10.1021/jf1006209

Cited by 77 publications

(34 citation statements)

References 17 publications

(18 reference statements)

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“…Although it is simple, rapid, and economical, some samples still require complicated pretreatment. An alternative method of competitive enzyme-linked immunosorbent assay 26 avoids the complex pretreatment, but needs to be operated by a trained professional. All of these MEL detection methods do not meet the requirements for onsite and in-home testing.…”

Section: Introductionmentioning

confidence: 99%

Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Wang¹,

Zhi²,

Zhao³

et al. 2014

IJN

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Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory method of detecting MEL in onsite or in-home testing is in urgent need of development. This work aimed to explore a rapid, convenient, and cost-effective method of identifying MEL in milk products or other food by colloidal selenium-based lateral flow immunoassay. Colloidal selenium was synthesized by L-ascorbic acid to reduce seleninic acid at room temperature. After conjugation with a monoclonal antibody anti-MEL, a test strip was successfully prepared. The detection limit of the test strip reached 150 μg/kg, 1,000 μg/kg, and 800 μg/kg in liquid milk, milk powder, and animal feed, respectively. No cross-reactions with homologues cyanuric acid, cyanurodiamide, or ammelide were found. Moreover, the MEL test strip can remain stable after storage for 1 year at room temperature. Our results demonstrate that the colloidal selenium MEL test strip can detect MEL in adulterated milk products or animal feed conveniently, rapidly, and sensitively. In contrast with a colloidal gold MEL test strip, the colloidal selenium MEL test strip was easy to prepare and more cost-efficient.

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Section: Introductionmentioning

confidence: 99%

Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Wang¹,

Zhi²,

Zhao³

et al. 2014

IJN

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“…Thus, the sensitive and accurate detection of melamine is of the utmost importance for food safety. Recently, some new technologies, such as high performance liquid chromatography (HPLC) [45], gas chromatography in tandem with mass spectrometry [46] and enzyme-linked immunosorbent assay (ELISA) [47] are employed for the detection of melamine. SERS has emerged as a more rapid and cost-effective method for the identification of adulterants in foods.…”

Section: Resultsmentioning

confidence: 99%

Chemical and Biological Sensing Using Diatom Photonic Crystal Biosilica With In-Situ Growth Plasmonic Nanoparticles

Kong

Squire

et al. 2016

IEEE Trans.on Nanobioscience

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In this paper, we described a new type of bioenabled nano-plasmonic sensors based on diatom photonic crystal biosilica with in-situ growth silver nanoparticles and demonstrated label-free chemical and biological sensing based on surface-enhanced Raman scattering (SERs) from complex samples. Diatoms are photosynthetic marine micro-organisms that create their own skeletal shells of hydrated amorphous silica, called frustules, which possess photonic crystal-like hierarchical micro-& nanoscale periodic pores. Our research shows that such hybrid plasmonic-biosilica nanostructures formed by cost-effective and eco-friendly bottom-up processes can achieve ultra-high limit of detection for medical applications, food sensing, water/air quality monitoring and geological/space research. The enhanced sensitivity comes from the optical coupling of the guided-mode resonance of the diatom frustules and the localized surface plasmons of the silver nanoparticles. Additionally, the nanoporous, ultra-hydrophilic diatom biosilica with large surface-to-volume ratio can concentrate more analyte molecules to the surface of the SERS substrates, which can help to detect biomolecules that cannot be easily adsorbed by metallic nanoparticles.

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“…ELISA as a screening method now has been developed rapidly (Garber 2008), but false positive results and high LOD weaken its accuracy. Previous publications indicated that the LODs of ELISA were 0.1 mg L À1 in milk, 0.2 mg L À1 in milk powder, 0.5 mg L À1 in feeds (Yin et al 2010) and 20 mg mL À1 in pet-food (Kim et al 2008) for the detection of melamine, respectively. Compared to instrumental methods [LOD, 0.002 mg kg À1 in GC-MS method (Xia et al 2009)], sensitivity of immunoassay still needs to be further improved.…”

Section: Rapid and Sensitive Fluoroimmunoassay Based On Quantum Dots mentioning

confidence: 98%

Rapid and Sensitive Fluoroimmunoassay Based on Quantum Dots for Detection of Melamine in Milk

Zhu

et al. 2013

Analytical Letters

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A rapid and sensitive indirect competitive fluorescence-linked immunosorbent assay (icFLISA) method was developed for detection of melamine in milk based on secondary antibody (Ab 2 )-conjugated quantum dots (QDs). The sensitivity of icFLISA with a limit of detection (LOD) of melamine (3.88 ng mL À1 ) and 50% inhibition value (IC 50 ) (31.58 ng mL À1 ), was much higher than that of icELISA. As a proof of principle, recovery tests involving four fortification levels (150, 80, 40, and 20 ngmL À1 ) in blank samples were also performed. The recoveries ranged from 80.85 to 110.54% with coefficient of variations (CVs) of 2.82-8.82%. When challenged with authentic samples, these results of icFLISA were consistent with that of icELISA, immuno-chromatographic assay and gas chromatography-single quadruple mass spectrometry (GC-MS)method, indicating that icFLISA can be considered as a new screening method for detection of melamine in milk.

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Preparation of Monoclonal Antibody for Melamine and Development of an Indirect Competitive ELISA for Melamine Detection in Raw Milk, Milk Powder, and Animal Feeds

Cited by 77 publications

References 17 publications

Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Chemical and Biological Sensing Using Diatom Photonic Crystal Biosilica With In-Situ Growth Plasmonic Nanoparticles

Rapid and Sensitive Fluoroimmunoassay Based on Quantum Dots for Detection of Melamine in Milk

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