Preparation of molecularly imprinted polymers and application in a biomimetic biotin-avidin-ELISA for the detection of bovine serum albumin

Chen, Guoning; Guo, Pengqi; Yan, Wei; Wang, Lu; Shu, Hua; Li, Yunzhe; Jing, Wanghui; Chang, Chun; Fu, Qiang

doi:10.1016/j.talanta.2019.01.088

Cited by 42 publications

(13 citation statements)

References 34 publications

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“…To overcome this problem, recently, molecularly imprinted polymers (MIPs) have been introduced as another receptor of MAMP to create binding site templates in synthetic polymers using molecular imprints or patterns (Karimi-Maleh et al, 2021b). Since the MIPs present some valuable features such as low cost, acceptable selectivity, reusability and easy preparation process, they are chosen for the sensor fabricate (Guoning et al, 2019;Haupt 2001;Haupt and Mosbach 2000). Graphene quantum dots (GQDs), as another nano-fluore tag, is a fantastic probe with amazing optical properties (Razmi and Mohammad-Rezaei 2013;Shen et al, 2011).…”

Section: Fluorimetric Sensorsmentioning

confidence: 99%

Recent advances in developing optical and electrochemical sensors for analysis of methamphetamine: A review

et al. 2021

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The origin of MAMP and its side effects have been reported. The optical and electrochemical sensors for sensing MAMP have been reviewed. The advantages and drawbacks of the applied modifiers and interfaces have been described. The undeniable role of nanotechnology in the expansion of the MAMP sensors has been described. Some offers for commercializing of MAMP sensors for the rutin analysis with low cost have been proposed.

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Section: Fluorimetric Sensorsmentioning

confidence: 99%

Recent advances in developing optical and electrochemical sensors for analysis of methamphetamine: A review

et al. 2021

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“…In this context, several efforts have been already devoted to discovering new signal reporters to increase ELISA sensitivity [3] and to pinpoint capturing agents, i.e., mimetics [8], able to flank and/or supplant antibodies that generally are expensive and sensitive to environmental conditions. Looking at mimetic capturing agents, the literature has highlighted aptamers and molecularly imprinted polymers (MIPs) as the most promising ones to develop diagnostic "antibody-free" ELISA-like format assays, i.e., enzymelinked oligonucleotide assay (ELONA) [6,9], biomimetic enzyme-linked immunoassay (BELISA) [10][11][12][13][14][15][16][17][18][19], pseudo-ELISA [7,[20][21][22][23], or nanoparticle-based assay (MINA) [1,15,[24][25][26][27][28][29]. At present, a positive imbalance of the literature is in favor of aptamers, with only a few reports about MIPs starting from 2014.…”

Section: Introductionmentioning

confidence: 99%

A biomimetic enzyme-linked immunosorbent assay (BELISA) for the analysis of gonadorelin by using molecularly imprinted polymer-coated microplates

et al. 2022

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An original biomimetic enzyme-linked immunoassay (BELISA) to target the small peptide hormone gonadorelin is presented. This peptide has been recently listed among the substances banned in sports by the World Antidoping Agency (WADA) since its misuse by male athletes triggers testosterone increase. Hence, in response to this emerging issue in anti-doping controls, we proposed BELISA which involves the growth of a polynorepinephrine (PNE)–based molecularly imprinted polymer (MIP) directly on microwells. PNE, a polydopamine (PDA) analog, has recently displayed impressive performances when it was exploited for MIP preparation, giving even better results than PDA. Gonadorelin quantification was accomplished via a colorimetric indirect competitive bioassay involving the competition between biotinylated gonadorelin linked to the signal reporter and the unlabeled analyte. These compete for the same MIP binding sites resulting in an inverse correlation between gonadorelin concentration and the output color signal (λ = 450 nm). A detection limit of 277 pmol L−1 was achieved with very good reproducibility in standard solutions (avCV% = 4.07%) and in urine samples (avCV% = 5.24%). The selectivity of the assay resulted adequate for biological specimens and non-specific control peptides. In addition, the analytical figures of merit were successfully validated by mass spectrometry, the reference anti-doping benchtop platform for the analyte. BELISA was aimed to open real perspectives for PNE-based MIPs as alternatives to antibodies, especially when the target analyte is a poorly or non-immunogenic small molecule, such as gonadorelin. Graphical abstract

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“…Therefore, the separation, purification and detection of proteins are particularly important. As a new type of protein identification and separation material, protein imprinted polymers have the advantages of easy preparation, low cost, high selectivity, strong practicability and promising mechanical stability, so it is widely favored by researchers [3][4][5] . After a prolonged endeavor, in recent years, the imprinting methods for proteins and other types of macromolecular templates have made some research progress [6][7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Anti-nonspecific adsorption segments-assisted self-driven surface imprinted fibers for efficient protein separation

Yang

Wang

et al. 2021

Preprint

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At present, the development of high-performance protein imprinted materials is still a research hotspot in the field of protein imprinting. Herein, anti-protein adsorption segment (CBMA)-assisted self-driven BSA surface imprinted fibers MTCFs@SIP@CBMA with high recognition selectivity are pioneered using the strategies of combining magnetic nanomaterial surface imprinting technique with amino-Michael addition. The special structure of the carrier MTCFs endows MTCFs@SIP@CBMA with magnetic performance and self-driven adsorption performance, which simplifies the separation process while improving the adsorption capacity and accelerating the adsorption rate. The adsorption capacity for BSA reached 395.26 mg/g within 30 min. The introduction of CBMA segments on the surface after imprinting by amino-Michael addition makes its polymer chain length and position controllable. Under the strongest anti-nonspecific adsorption effect, MTCFs@SIP@CBMA exhibit excellent specific identification to BSA from mixed proteins. Additionally, MTCFs@SIP@CBMA show considerable reusability. Therefore, MTCFs@SIP@CBMA are expected to be applied in efficient separation of proteins in biological samples.

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Preparation of molecularly imprinted polymers and application in a biomimetic biotin-avidin-ELISA for the detection of bovine serum albumin

Cited by 42 publications

References 34 publications

Recent advances in developing optical and electrochemical sensors for analysis of methamphetamine: A review

Recent advances in developing optical and electrochemical sensors for analysis of methamphetamine: A review

A biomimetic enzyme-linked immunosorbent assay (BELISA) for the analysis of gonadorelin by using molecularly imprinted polymer-coated microplates

Anti-nonspecific adsorption segments-assisted self-driven surface imprinted fibers for efficient protein separation

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