Preparation of Modified RNA Sequences for Biological Research

Abstract

Organic chemistry Z 0200 Preparation of Modified RNA Sequences for Biological Research -[25 refs.]. -(PITSCH, S.; ACKERMANN, D.; DENARIE, C.; MEYLAN, F.; MEYYAPPAN, M.; MULLER, E.; PEER, A.; PORCHER, S.; REYMOND, L.; STUTZ, A.; et al.; Chimia 59 (2005) 11, 808-811; Lab. Nucleic Acid Chem., Ec. Polytech. Fed. Lausanne, CH-1015 Lausanne, Switz.; Eng.) -Lindner 30-273

In vitro Transcription and Purification of RNAs of Different Size

“…The chemical synthesis of RNA sequences has been described in detail [9] andisusuallycarriedoutbycompaniesor in specialized laboratories [10]. Standardized chemical synthesis of RNA is one of the significant achievements in RNA (bio)chemistry of the past few years and is the method of choice in the following cases: (i) ifshortsequences(<15nt)areneeded, whichareusuallydifficulttoobtainin good yield and purity by transcription; (ii) ifaspecificsequenceatthe5ʹ-endof the RNA is required that cannot be obtainedbyinvitrotranscription(see alsobelow); (iii)ifnon-standardnucleotidederivatives have to be incorporated site-specifically into the sequence.…”