Preparation of micro-biosensor for continuous glucose monitoring

Abstract

Amperometric glucose sensors with a diameter of less than 10 µm were fabricated by the immobilization of glucose oxidase (GOx) on carbon fiber electrodes. Carbon fiber electrodes with platinum thin film were also prepared using electroplating and sputtering. The combination of electrodeposition and electropolymerization was employed for the immobilization of GOx. Although the introduction of Pt film satisfactorily improved the oxidation current of the electrode to hydrogen peroxide, sensitivities of obtained GOx-immobilized electrodes to glucose were not significant. Formation of electroplatedplatinum thin film on carbon fiber was effective to reduce the influence of electroactive compounds existing in biological fluid such as ascorbic and uric acids.