Preparation of methacrylamide grafted and dye‐ligand immobilized PET fibers: Studies of adsorption and purification of lysozyme

Abstract

In this study, novel affinity chromatographic fibers was prepared from methacrylamide grafted poly(ethylene terephthalate), PET-g-pMAA, using benzoylperoxide as an initiator. A dye ligand (i.e., Procion Brown) as a ligand was then covalently immobilized on the different amount of pMAAm grafted PET fibers, (PET-gpMAAm-PB). The fibers were characterized by surface area measurement, infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). Adsorptive properties of the composite fibers were tested using a model protein (i.e., lysozyme). To achieve these purposes, the influence of pH, ionic strength, initial lysozyme concentration, and temperature on adsorption system has been investigated and evaluated. A maximum lysozyme adsorption PET-g-pMAAm-PB fiber was obtained as 43.9 mg g 21 at pH 7.5. The experimental equilibrium data obtained for lysozyme adsorption onto PET-g-pMAAm-PB fibers fitted well to the Langmuir isotherm model. The result of kinetic analyzed for lysozyme adsorption onto affinity fibers showed that the secondorder rate equation was favorable. The purity of the eluted lysozyme, as determined by HPLC, was 84% with recovery 73% for PET-g-pMAAm-PB fiber. Experiments on regeneration and dynamic adsorption were also performed. It appears that PET-g-pMAAm-PB fibers can be applied for lysozyme separation without causing any denaturation.

Poli(Hidroksietilmetakrilat-Genişletilmiş Perlit) Kompoziti ile Yumurta Akından Lizozimin Tek Basamakta Saflaştırılması

“…Chicken egg white's protein components were ovalbumin (pI:4.5), ovotransferrin (pI:6.1), ovomucoid (pI:4.5) and lysozyme (pI:10.7) [30,31]. The specific activity of the purified lysozyme with P(Hema-Ep) composite was 50 U mg -1 which was reported in literature [32].…”

Polymer Composites Based on Glycol-Modified Poly(Ethylene Terephthalate) Applied to Additive Manufacturing Using Melted and Extruded Manufacturing Technology

“…Poly(ethylene terephthalate) also turned out to be an interesting material for 3D printing. Currently, a lot of emphasis has been placed on the correction of the properties of PET fibers, which is why filaments are most often made of a glycol-modified version of the polymer (PET-G) [18]. Modification of the material with glycol allows fibers to be obtained that are less brittle, easier to print, and more transparent in the case of transparent versions of this material.…”

Preparation of hydrophilic woven fabrics: Surface modification of poly(ethylene terephthalate) by grafting of poly(vinyl alcohol) and poly(vinyl alcohol)‐g‐(N‐vinyl‐2‐pyrrolidone)

“…To remedy the unwanted properties of PET fiber and gain new desired functional groups, and consequently, improve its usage in different fields, researchers have concentrated on modification of its surface with physical and chemical methods . Among these methods, chemical modification methods such as plasma processing, graft copolymerization (grafting to polymers and/or grafting from monomers), crosslinking, enzymatic treatment, and aminolysis have been frequently preferred to modify the surface of fibrous PET in the literature. Among them, the graft copolymerization has been one of the most preferred methods in the surface chemical modification, owing to enabling the inclusion of new functional groups to the surface of PET, by requiring of less energy consumption and low‐cost chemicals/apparatus.…”